Long-Term Antidepressant Treatment Inhibits Neuropathic Pain-Induced CREB and PLCγ-1 Phosphorylation in the Mouse Spinal Cord Dorsal Horn

Kusuda, Ricardo; Ravanelli, Maria Ida Bonini; Cadetti, F.; Franciosi, Adriano C.; Previdelli, Karina; Zanon, Sonia; Lucas, Guilherme

doi:10.1016/j.jpain.2013.04.015

Cited by 10 publications

(11 citation statements)

References 54 publications

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“…Phospholipase C γ 1 (PLCG1) belongs to the phospholipase C superfamily and plays an important role in the synthesis of two key secondary messengers, inositol 1,4,5‐trisphosphate and diacylglycerol. PLCG1 has been reported to be associated with reproduction as well as with anxiety‐related behaviour, and it has an anti‐depressive effect in neuronal systems (Giusti, Carroll, Abassi, & Foltz, ; Kusuda et al., ; Sette, Bevilacqua, Geremia, & Rossi, ). Calcium/calmodulin‐dependent protein kinase type II β chain (CAMK2b) belongs to the serine/threonine protein kinase superfamily, and CAMK2b activity in the limbic system has been shown to be associated with the regulation of behaviour and stress responses (Novak, Seeman, & Tallerico, ; Runft, Watras, & Jaffe, ; van Woerden et al., ).…”

Section: Discussionmentioning

confidence: 99%

Hypothalamic DNA methylation in rats with dihydrotestosterone‐induced polycystic ovary syndrome: effects of low‐frequency electro‐acupuncture

Cui

Tamadon

et al. 2018

Experimental Physiology

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Polycystic ovary syndrome (PCOS) is a common reproductive and endocrine disease of unknown aetiology. Recently, epigenetic studies focusing on DNA methylation in PCOS have received much attention, but the mechanisms are still unclear. In the present study, we used the 5α-dihydrotestosterone-induced PCOS-like rat model and treated the rats with electro-acupuncture (EA). Rats were randomly divided into four groups - controls, diet-induced obesity, PCOS and PCOS+EA. We examined the reproductive, metabolic and behavioural phenotypes, validated the effect of EA, and explored the role of hypothalamic DNA methylation by analysing the methylation of global DNA and selected candidate genes. The PCOS rats presented with reproductive dysfunctions such as lack of regular oestrous cyclicity, metabolic disorders such as increased body weight and insulin resistance, and depression and anxiety-like behaviours. EA improved the reproductive functions, decreased body weight and improved experimental depressive behaviour. Furthermore, global DNA methylation and the expression of DNA methyltransferases (DNMTs) were increased in PCOS rats compared to the control group, and EA decreased the global DNA methylation and the expression of DNMT3b. In addition, pyrosequencing showed that the DNA methylation of certain CpG sites in targeted genes (Plcg1, Camk2b, Esr2 and Pgr) was increased in the PCOS group, but the DNA methylation of Camk2b and Ar was decreased after EA treatment. These results indicate that hypothalamic DNA methylation might be correlated with the development of PCOS and that EA has an effect on hypothalamic DNA methylation in PCOS rats.

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Section: Discussionmentioning

confidence: 99%

Hypothalamic DNA methylation in rats with dihydrotestosterone‐induced polycystic ovary syndrome: effects of low‐frequency electro‐acupuncture

Cui

Tamadon

et al. 2018

Experimental Physiology

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“…Protein concentration in tissue homogenates was determined by a modified Lowry assay (DC Protein Assay, Bio-Rad, Hercules, CA, USA). These procedures were previously described in part [27,28]. Proteins were denatured by boiling in Laemmli sample buffer containing 100 mM DTT, run on SDS-PAGE gel and transferred to PVDF membranes (Amersham Biosciences, Piscataway, NJ, USA).…”

Section: Sample Collection and Protein Analysis By Immunoblottingmentioning

confidence: 99%

Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords

et al. 2015

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The aim of this investigation was to evaluate the effects of 3 overtraining (OT) protocols on the glial activation and apoptosis in the spinal cords of mice. Rodents were divided into control (C; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR). The incremental load test, ambulation test, exhaustive test and functional behavioural assessment were used as performance evaluation parameters. 36 h after the exhaustive test, the dorsal and ventral parts of the lumbar spinal cord (L4-L6) were dissected for subsequent protein analysis by immunoblotting. The OT protocols led to similar responses of some performance parameters. The ventral glial fibrillary acidic protein (GFAP) protein levels were diminished in the OTR/up and OTR compared to CT and OTR/down groups. The ventral ionized calcium binding adaptor molecule 1 (Iba-1), and the dorsal GFAP and Iba-1 protein levels were increased in the OTR/down compared to the other groups. The ratio between the cleaved capase-3/caspase-3 and cleaved caspase-9/caspase-9 measured in the spinal cord were not sensitive to the OT protocols. In summary, the OTR/down activated the glial cells in the motor (i. e. Iba-1) and sensory (i. e. GFAP and Iba-1) neurons without leading to apoptosis.

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“…The clinical study by Schliessbach et al (2018) shows that single-dose imipramine does not provide a satisfactory analgesic effect in comparison with the placebo, probably because of insufficient dose or non-effectiveness of imipramine [18]. Preclinical study reveals TCAs' analgesic onset does not appear immediately after acute used but is delayed following 7-14 days of chronically used [22,[26][27][28]. TCAs need time to influence the occurrence of molecular plasticity, hence, the onset of analgesic effect delays for several days after the first treatment [22,28].…”

Section: Discussionmentioning

confidence: 99%

Tricyclic Antidepressants in Chronic Low Back Pain : A Review

Ilmanita

Hidayati

2020

J. Islamic Pharm.

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Chronic low back pain (LBP) is the frequent chronic pain and notable health problem which is the main reason of disabling conditions, individual suffering, sick leave, and costly treatment. Unfortunately, available therapy especially oral medicines only beneficial in no more than 50% of patients. Tricyclic antidepressants (TCAs) are commonly used for neuropathic pain but the data for neuropathic LBP is limited. The purpose of this review is to evaluate the efficacy and adverse drug reaction of TCAs in chronic LBP. We searched scientific search engines including Scopus and PubMed and collected original articles including Randomized Clinical Trial (RCT), comparative, and observational study about Tricyclic Antidepressants treatment in chronic LBP from 1998 to 2019. In this review, 5 articles and 543 patients were evaluated. All recruited articles are randomized clinical trials. Among all five relevant articles, three of them evaluate amitriptyline treatment in chronic LBP while the rest articles evaluate imipramine and nortriptyline. Amitriptyline is the most well-studied TCA for chronic LBP. We found that both amitriptyline and nortriptyline can reduce pain severity significantly when the dose is increased gradually depending on patient tolerance. TCAs can also improve disability significantly. However, single-dose imipramine is not superior than placebo. We conclude that TCAs can be effective for treating chronic LBP when used routinely for several weeks and the dose should be increased gradually depending on patient tolerance although further studies are required to confirm the optimal dose for analgesia. The most prevalence Adverse Drug Reactions (ADRs) of TCA including dry mouth, somnolence, insomnia, sedation, and constipation.

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Long-Term Antidepressant Treatment Inhibits Neuropathic Pain-Induced CREB and PLCγ-1 Phosphorylation in the Mouse Spinal Cord Dorsal Horn

Cited by 10 publications

References 54 publications

Hypothalamic DNA methylation in rats with dihydrotestosterone‐induced polycystic ovary syndrome: effects of low‐frequency electro‐acupuncture

Hypothalamic DNA methylation in rats with dihydrotestosterone‐induced polycystic ovary syndrome: effects of low‐frequency electro‐acupuncture

Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords

Tricyclic Antidepressants in Chronic Low Back Pain : A Review

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