Long-term continuous monitoring of microRNA in living cells using modified gold nanoprobe

Liang, Yuling; Yang, Huihui; Yin, Wen; Zhang, Yanfei; Xu, Yuzhi; Liu, Siyang; Dai, Zong; Zou, Xiaoyong

doi:10.1007/s00216-022-04182-5

Cited by 5 publications

(1 citation statement)

References 37 publications

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“…While the design and utilization of oligonucleotide chains as probes are widespread in non-living cell environments, their stability within cellular environments is notably lacking. To address this limitation, researchers currently rely on linear nucleotide chains as the foundational design, employing methodologies such as HCR and CHA to engineer more intricate binding chains or construct spatial DNA structures to attain added functionality [64 , [73] , [74] , [75] . Further details on these advancements will be discussed in the following chapters.…”

Section: Targeting Unitmentioning

confidence: 99%

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Liu,

Shi,

et al. 2024

Asian Journal of Pharmaceutical Sciences

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Section: Targeting Unitmentioning

confidence: 99%

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Liu,

Shi,

et al. 2024

Asian Journal of Pharmaceutical Sciences

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Live‐Cell Imaging of MicroRNA Expression via Photoinduced Electron Transfer Controlled by Catalytic Hairpin Assembly

Na,

Koo,

Park

et al. 2024

Adv Healthcare Materials

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MicroRNAs (miRNAs) serve as emerging biomarkers for a range of diseases, and their quantitative analysis draws increasing attention. Yet, current invasive methods limit continuous tracking within living cells. To overcome this, a nonenzymatic DNA‐based nanoprobe is developed for dynamic, noninvasive miRNA tracking via live‐cell imaging. This probe features a unique hairpin DNA structure with five guanines that act as internal quenchers, suppressing fluorescence from an attached fluorophore via photoinduced electron transfer. Target miRNA initiates toehold‐mediated strand displacement, restoring, and amplifying the fluorescence signal. Additionally, by introducing a single mismatch to the hairpin DNA, the nanoprobe's sensitivity is significantly enhanced, lowering the detection limit to about 60 pM without compromising specificity. To optimize intracellular delivery for prolonged monitoring, the nanoprobe is encapsulated within multilamellar lipid nanovesicles, fluorescently labeled for dual‐wavelength ratiometric analysis. The proposed nanoprobe demonstrates a significant advance in live‐cell miRNA detection, promising enhanced in situ analysis for a better understanding of miRNAs’ pathophysiological function.

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Magnetic beads and GO-assisted enzyme-free signal amplification fluorescent biosensors for disease diagnosis

Xu,

Gui,

et al. 2024

Analytica Chimica Acta

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Long-term continuous monitoring of microRNA in living cells using modified gold nanoprobe

Cited by 5 publications

References 37 publications

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

Live‐Cell Imaging of MicroRNA Expression via Photoinduced Electron Transfer Controlled by Catalytic Hairpin Assembly

Magnetic beads and GO-assisted enzyme-free signal amplification fluorescent biosensors for disease diagnosis

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