Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids

Biofilm cultivation of marine dinoflagellates under different temperatures and nitrogen regimes enhances DHA productivity

“…In the present study, the maximal DHA productivity for S. microadriaticum CCAC 2475 B after 4 days of nitrogen depletion in a TL-PSBR was recorded to be 145.4 mg m − 2 day − 1 . Although maximal DHA content in the dry biomass (2% in a two-phase experiment in TL-PSBR) has not yet been optimized for S. microadriaticum CCAC 2475 B and is lower than that reported for other autotrophic dinoflagellates grown in suspension (3.4-3.5% for a long-term semicontinuous culture in an indoor raceway PBR (Molina-Miras et al 2018;Peltomaa et al 2019), because of the higher biomass productivity in the TL-PSBR compared to suspension cultivation (6.3 g m −2 d −1 compared to 2.5 g m −2 d −1 in Molina-Miras et al 2018), the DHA productivity recorded here compares favourably with previous reports. It should be noted that no external addition of CO 2 was used in this study; addition of 2% CO 2 has recently been shown to enhance biomass productivity in S. voratum CCAC 0047 (Langenbach and Melkonian 2019).…”

“…Dinoflagellates contain high levels of omega-3 PUFAs, especially DHA. Their cultivation in suspension, however, suffers from low growth rates and is frequently hampered by their sensitivity to shear stress (Dixon and Syrett 1988;Gibson and Thomas 1995;Gallardo-Rodríguez et al 2009;López-Rosales et al 2015;Wang and Lan 2018), although continuing efforts are being made to address these issues using either open or closed cultivation systems (Fuentes-Grünewald et al 2016;Molina-Miras et al 2018;López-Rodríguez et al 2019). Cell immobilization using a twin-layer porous substrate photobioreactor (TL-PSBR) was recently applied successfully to the peridinin-producing marine dinoflagellate Symbiodinium voratum, which resulted in much higher growth rates compared to a suspension culture (Benstein et al 2014;Langenbach and Melkonian 2019).…”

Data on the Amphidinium carterae Dn241EHU isolation and morphological and molecular characterization

“…The strain, used in a study for production of carotenoids and fatty acids [1] , was isolated from Punta des Gas (Mallorca, Spain) (39°33′50″ N - 2°39′20″ E) in October 2013, by mouth pipetting. This technique allows isolating a unique cell to obtain clonal cultures and it is very effective with cells with no very quick movement.…”

“…The morphological characterization was made using two different techniques of microscopy and the molecular characterization by using the 28S rDNA sequences of D1 and D2 domains. This strain has been used for a culture study in an indoor LED-lighted pilot-scale raceway to determine its production of carotenoids and fatty acids, “Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids.” (Molina-Miras et al, 2018) [1] .…”

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

“…The total phosphorous and nitrogen contained in the biomass and supernatants were determined as phosphate (4500-P) and nitrate (4500-N) after applying a modified chemical wet-oxidation method, as previously reported (Molina-Miras et al, 2018). The protein content in the biomass was measured, as described by González-López et al (2010).…”