Long-term effects of knitted silk–collagen sponge scaffold on anterior cruciate ligament reconstruction and osteoarthritis prevention

Shen, Wei; Chen, Xiao; Hu, Yin; Yin, Zi; Zhu, Ting; Hu, Jiajie; Chen, Jialin; Zheng, Zefeng; Zhang, Wei; Ran, Jisheng; Heng, Boon Chin; Ji, Junfeng; Chen, Weishan

doi:10.1016/j.biomaterials.2014.06.019

Cited by 94 publications

(51 citation statements)

References 36 publications

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“…Pre-seeding scaffolds with mesenchymal stem cells (MSCs) dramatically increased tissue regeneration, collagen deposition and blood vessel growth within a scaffold used for ACL repair (74). A similar increase in tissue growth and collagen deposition was seen in silk scaffolds pre-seeded with tendon stem/progenitor cells (TSPCs) used for rotator cuff repair (75). The TSPCs also caused a decrease in the inflammatory response with more fibroblasts and less lymphocytes growing within the scaffold as compared to unseeded controls.…”

Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 75%

“…Thus, combining the mechanical benefits of silk with the cell compatibility benefits of collagen can make an ideal blend for many tissue engineering applications. Indeed, silk-collagen scaffolds greatly improved tendon reconstruction (27, 75, 76). Silk-collagen scaffolds had significantly increased regenerated tissue and organized collagen fibers compared to silk only scaffolds (75).…”

Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 99%

“…Indeed, silk-collagen scaffolds greatly improved tendon reconstruction (27, 75, 76). Silk-collagen scaffolds had significantly increased regenerated tissue and organized collagen fibers compared to silk only scaffolds (75). The silk-collagen scaffolds also had increased vasculature at 2 months post surgery, which is an integral aspect of tendon healing and likely contributed greatly to the improved phenotypes seen at later time points.…”

Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 99%

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In vivo bioresponses to silk proteins

2015

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Silks are appealing materials for numerous biomedical applications involving drug delivery, tissue engineering, or implantable devices, because of their tunable mechanical properties and wide range of physical structures. In addition to the functionalities needed for specific clinical applications, a key factor necessary for clinical success for any implanted material is appropriate interactions with the body in vivo. This review summarizes our current understanding of the in vivo biological responses to silks, including degradation, the immune and inflammatory response, and tissue remodeling with particular attention to vascularization. While we focus in this review on silkworm silk fibroin protein due to the large quantity of in vivo data thanks to its widespread use in medical materials and consumer products, spider silk information is also included if available. Silk proteins are degraded in the body on a time course that is dependent on the method of silk fabrication and can range from hours to years. Silk protein typically induces a mild inflammatory response that decreases within a few weeks of implantation. The response involves recruitment and activation of macrophages and may include activation of a mild foreign body response with the formation of multinuclear giant cells, depending on the material format and location of implantation. The number of immune cells present decreases with time and granulation tissue, if formed, is replaced by endogenous, not fibrous, tissue. Importantly, silk materials have not been demonstrated to induce mineralization, except when used in calcified tissues. Due to its ability to be degraded, silk can be remodeled in the body allowing for vascularization and tissue ingrowth with eventual complete replacement by native tissue. The degree of remodeling, tissue ingrowth, or other specific cell behaviors can be modulated with addition of growth or other signaling factors. Silk can also be combined with numerous other materials including proteins, synthetic polymers, and ceramics to enhance its characteristics for a particular function. Overall, the diverse array of silk materials shows excellent bioresponses in vivo with low immunogenicity and the ability to be remodeled and replaced by native tissue making it suitable for numerous clinical applications.

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Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 75%

Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 99%

Section: Vascular Ingrowth Into Silk Materialsmentioning

confidence: 99%

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In vivo bioresponses to silk proteins

2015

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“…The common aspect of these approaches is the preparation of braided, knitted, or yarned fibrous structures that serve as a frame for further production. In some cases, this frame of future material can be elaborated prior to degumming, still most of all the subsequent treatments are performed after sericin removal step. Bromination and chlorination of SF fabrics on Ser residues with halogen derivatives were reported, with yields of Ser conversion of 10 and 30%, respectively.…”

Section: Manipulating Silk Fibroin: Initial Processing and Physical Tmentioning

confidence: 99%

On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

Volkov

Ferreira

Cavaco-Paulo

2015

Macromol. Mater. Eng.

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For years, silk fibroin of a domestic silkworm, Bombyx mori, has been recognized as a valuable material and extensively used. In the last decades, new application fields are emerging for this versatile material. Those final, specific applications of silk dictate the way it has been processed in industry and research. This review focuses on the description of various approaches for silk downstream processing in a laboratory scale, that fall within several categories. The detailed description of workflow possibilities from the naturally found material to a finally formulated product is presented. Considerable attention is given to (bio‐) chemical approaches of silk fibroin transformation, particularly, to its enzyme‐driven modifications. The focus of the current literature survey is exclusively on the methods applied in research and not industry.

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“…In addition to the extra regulatory and patient burden, once the cells are implanted, they die at an accelerated rate, typically with only a few percentage of the implanted cells found to survive more than a few weeks in the body. 41, 44, 55 This may be due to a relatively rapid change in environment for these cells, from the carefully monitored and controlled in vitro environment to an in vivo environment which may have significantly less nutrition or oxygen.…”

Section: Section One: a Review Of The Science Behind Bio-enhanced Aclmentioning

confidence: 99%

Bridge-Enhanced ACL Repair: A Review of the Science and the Pathway Through FDA Investigational Device Approval

Proffen

Perrone

Roberts³

et al. 2015

Ann Biomed Eng

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Injuries to the anterior cruciate ligament (ACL) are currently treated with replacement of the torn ligament with a graft of tendon harvested from elsewhere in the knee. This procedure, called "ACL reconstruction," is excellent for restoring gross stability to the knee; however, there are relatively high graft failure rates in adolescent patients,4, 12, 60 and the ACL reconstruction procedure does not prevent the premature osteoarthritis seen in patients after an ACL injury.1, 46, 52 Thus, new solutions are needed for ACL injuries. Researchers have been investigating the use of scaffolds, growth factors and cells to supplement a suture repair of the ACL (bio-enhanced repair). In this paper, we will review the varied approaches, which have been investigated for stimulating ACL healing and repair in preclinical models and how one of these technologies was able to move from promising preclinical results to FDA acceptance of an Investigational Device Exemption (IDE) application for a first-in-human study.

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Long-term effects of knitted silk–collagen sponge scaffold on anterior cruciate ligament reconstruction and osteoarthritis prevention

Cited by 94 publications

References 36 publications

In vivo bioresponses to silk proteins

In vivo bioresponses to silk proteins

On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

Bridge-Enhanced ACL Repair: A Review of the Science and the Pathway Through FDA Investigational Device Approval

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