Long-term following-up of viability of spleen autotransplants in the Beagle canine model

Abstract

<jats:p>PURPOSE: To examine the possible late complications of splenectomy or spleen autotransplantation in large laboratory animal model, in which we need non-invasive or minimal-invasive methods for long-term monitoring of the experimental animals. METHODS: Experimental groups of beagle dogs were: non-operated control, sham-operated control, splenectomy, spleen autotransplantation with 5 or 10 spleen-chips taken into the greater omentum (Furka's technique). Prior to operations, on the 1st postoperative week, monthly till the 6th as well as in the 9th and 12th month, hemorheological examinations were performed. In postoperative 12th month colloid scintigraphy and diagnostic laparoscopy were carried out. At the end of the investigation comparative morphological examinations were performed, too. RESULTS: From the 4th-5th postoperative month filtration function of spleen-autotransplants showed particular restoration compared to splenectomy group. However, the functional results did not reach the values of the control or sham-operated groups. Sham-operated control's scintigraphy nicely showed activity in the spleen. In spleen autotransplantation-groups scintigraphy indicated well the activity of spleen-chips. During diagnostic laparoscopy spleen-chips with their blood supply were found. Histologically, the structure of spleenautotransplants was similar to normal splenic tissue. CONCLUSIONS: The autotransplants are regenerated, their functions have been partly restored, and thus spleen autotransplantation may prevent the possible complications of splenectomy. These parameters and the presented investigative protocol are suitable for long-term following-up of viability of the spleen-autotransplants.</jats:p>

Is there a relationship between lipids metabolism and splenic surgeries?

“…In addition to these functions, the participation of the spleen in metabolic control is receiving increasing attention [1][2][3][4][5][6][7][8][9] .…”

“…The functions of the spleen are part of the organic metabolism and disorders of this organ are related to different diseases [1][2][3][4][5][6][7][8][9][10] and Fabry disease also present spleen manifestations 10 . The spleen participates in the metabolism of all metals, of albuminoids and of indirect bilirubin from phagocytized red blood cells 11,12 .…”

Preliminary study of coconut water for graft tissues preservation in transplantation

“…The scintigraphic images recorded the radiotracer uptake by splenic implants with difficulty due to the overlapping of liver image 13 . However, the emission of radiation dosages by implanted spleen fragments left no doubt that 12 weeks were sufficient for their phagocytic functional recovery 13,14 .…”

“…The scintigraphic images recorded the radiotracer uptake by splenic implants with difficulty due to the overlapping of liver image 13 . However, the emission of radiation dosages by implanted spleen fragments left no doubt that 12 weeks were sufficient for their phagocytic functional recovery 13,14 . There was no difference between the conservation of liquids studied in relation to the evaluation of the function of the splenic implants by scintigraphy, which is a more sensitive method to indicate uptake by spleen cells than the blood tests 14 .…”

Histological Changes of Spleen Autotransplanted in the Omentum of Rats

“…In clinical trials, Immunoprophylaxis, chemoprophylaxis and spleen preserving surgery such as resection of the spleen and splenic autotransplantation along with informing and training patients prevented many serious complications [7]. Some studies have suggested the autotransplantation of homogenized spleen tissue.…”