Long-term Treatment with Hesperidin Improves Endothelium-dependent Vasodilation in Femoral Artery of Spontaneously Hypertensive Rats: The Involvement of NO-synthase and K_vChannels

Abstract

Hesperidin is the most common flavonoid found in citrus fruits and is expected to exert vasodilation action relevant to its health benefits. The present study aimed to explore the effect of hesperidin on the vascular responses in normotensive and hypertensive rats and the involvement of NO-synthase and K channels. The 15-week-old Wistar and spontaneously hypertensive rats (SHR) were randomized to orally receive either hesperidin (50 mg/kg/day) or a corresponding volume of the water for 4 weeks. Vascular responses of isolated femoral arteries were studied with myograph in control conditions and during inhibition of NO-synthase with l-NNA and K channels with 4-AP. Hesperidin had no effect on blood pressure. Endothelium-dependent vasodilation in Wistar and SHR was significantly improved by the treatment with hesperidin. The contraction responses after l-NNA were increased in all groups of rats to similar extent, but relaxatory responses were significantly attenuated only in SHR. The inhibition of K channels significantly reduced endothelium-dependent vasodilatory responses in only in SHR administered with hesperidin. The results of our experiment indicate that hesperidin might improve the endothelium-dependent vasodilation during hypertension, possibly through the enhancement of K channels function. Copyright © 2016 John Wiley & Sons, Ltd.

Blood pressure lowering and anti‐inflammatory effects of hesperidin in type 2 diabetes; a randomized double‐blind controlled clinical trial

“…Hesperidin and its metabolites have been reported to have the ability to control the vascular tone and exert hypotensive effects in experimental models; both short‐term and long‐term administration of hesperidin and its analogues and metabolites had antihypertensive effects (Dobiaš, Petrová, Vojtko, & Kristová, ; Ohtsuki et al, ). Bovine aortic endothelial cells treatment with hesperetin (active aglycone form of hesperidin) stimulated phosphorylation of enzymes that mediate nitric oxide (NO) production in vascular endothelial cells (Rizza et al, ).…”

Pharmacological Actions of Citrus Species

“…The inhibition of the potassium channels (Kv) significantly reduced the endothelium-dependent vasodilator response only in SHR rats administered hesperidin. This indicates that hesperidin can improve endothelium-dependent vasodilation during hypertension [56].…”

Acceleration in the Repair and Regenerative Responses by Different Doses of Hesperidin in the Deep Full Thickness Cutaneous Wound of Mice Whole Body Exposed To 6 Gy of γ -Radiation

“…The polyphenols, abundant in fruits and vegetables, have gained recognition for their antioxidant properties and their roles in protecting against chronic diseases such as cancer and cardiovascular diseases [33][34][35][36][37][38][39][40][41]46]. A wound is a discontinuity or break in skin associated with the disruption of structures and function of the underlying tissues and immediate steps are required by the wound tissue to restore the continuity and physiological functions to avoid any pathophysiological implications [31,47].…”

“…It prevents haemorrhages and helps in conditions of ruptured capillaries and connective tissues and builds a protective barrier against infections [33]. Hesperidin is an anti-cancerous, anti-microbial, antiallergenic, anti-hypotensive, anti-proliferative and a vasodilator [34][35][36][37][38][39]. Hesperidin has been found to be hypolipidemic in humans and retard bone loss [40,41].…”