Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions

Cavanagh, D.; Mawditt, K.; Britton, Paul; Naylor, C. J.

doi:10.1080/03079459994399

Cited by 178 publications

(168 citation statements)

References 24 publications

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“…RT-PCR has been extensively used for the molecular detection of IBV in allantoic fluid extracts and has been described as a very efficient, sensitive and accurate method for the detection and identification of IBV types (Cavanagh et al, 1999). Additionally, RT-PCR facilitates the detection of new and/or emerging IBV variants different antigenic properties due to alterations in the S1 subunit of the IBV spike glycoprotein (S).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

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Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

El-Rahman¹,

Hoffmann²,

Lueschow³

et al. 2015

Adv. Anim. Vet. Sci.

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| Infectious bronchitis virus (IBV) is the causative agent of an acute and highly contagious disease affecting the respiratory, renal and/or reproductive systems of chicken called infectious bronchitis disease. Controlling of this disease needs continuous identification of the circulating strains and genotypes. In the present study, six IBV samples were isolated from different poultry farms located in northern Egypt and propagated in the allantoic cavity of specific pathogen-free embryonated chicken eggs causing curling and dwarfing of embryos at different serial egg passages. The harvested allantoic fluids were titrated by chicken embryo kidney cell cultures selecting suitable viral concentrations for investigating the ciliostasis effect of IBV on tracheal organ cell cultures. In addition, the nucleotide and protein sequences of the IBV spike glycoprotein (S) were used for molecular relationship analysing and construction of phylogenetic trees. All examined viruses induced complete ciliostasis at the fifth day post inoculation. Isolates shared total sequence identities of 99-100% on nucleotide and amino acid levels. The Egyptian strain (Eg/CLEVB-1/IBV/012) showed 99% and the Israeli strain (IS/1494/06 IBV) showed 98% with the collected isolates and recognized as the local strains with the highest total sequence identities and phylogenetic relatedness of all strains available from the Gene Bank. The Italy02 strain of IBV is the nearest relative to the collected isolates with total sequence identities of 79 and 77% at nucleotide and amino acid levels, respectively. The new variant strains currently isolated from the northern of Northern Egypt should be evaluated periodically for developing suitable autogenously based virus vaccines.

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Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

“…July 2015 | Volume 3 | Issue 7 | Page 370 (Cavanagh et al, 1999). In this study TOCs were used for compering the infectivity of the recently detected Egyptian IBV isolates as well as reference strains (Beaudette and H120).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

El-Rahman¹,

Hoffmann²,

Lueschow³

et al. 2015

Adv. Anim. Vet. Sci.

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“…Oligonucleotide design was based on having a purine base (G or C) at the 3Ј terminus. These tests were tested alongside a slightly modified version of an established attachment protein G (test 5) PCR, 6,14 and all tests adopted the protocol of the latter. All oligonucleotide sequences are presented in Table 1.…”

mentioning

confidence: 99%

“…8,10 The application of PCR preceded by a reverse transcriptase (RT) step has led to increased levels of detection by several authors. 2,6,11,14,15,18 Tests have been used to both detect and differentiate APVs using primers designed in matrix (M), attachment (G), fusion (F), and nucleocapsid (N) protein genes. However, it still frequently proves difficult to detect viral nucleic acid in situations where the virus has been indicated using retrospective serology.…”

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confidence: 99%

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Design, Validation, and Absolute Sensitivity of a Novel Test for the Molecular Detection of Avian Pneumovirus

et al. 2004

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Abstract. This study describes attempts to increase and measure sensitivity of molecular tests to detect avian pneumovirus (APV). Polymerase chain reaction (PCR) diagnostic tests were designed for the detection of nucleic acid from an A-type APV genome. The objective was selection of PCR oligonucleotide combinations, which would provide the greatest test sensitivity and thereby enable optimal detection when used for later testing of field materials. Relative and absolute test sensitivities could be determined because of laboratory access to known quantities of purified full-length DNA copies of APV genome derived from the same A-type virus. Four new nested PCR tests were designed in the fusion (F) protein (2 tests), small hydrophobic (SH) protein (1 test), and nucleocapsid (N) protein (1 test) genes and compared with an established test in the attachment (G) protein gene. Known amounts of full-length APV genome were serially diluted 10-fold, and these dilutions were used as templates for the different tests. Sensitivities were found to differ between the tests, the most sensitive being the established G test, which proved able to detect 6,000 copies of the G gene. The G test contained predominantly pyrimidine residues at its 3Ј termini, and because of this, oligonucleotides for the most sensitive F test were modified to incorporate the same residue types at their 3Ј termini. This was found to increase sensitivity, so that after full 3Ј pyrimidine substitutions, the F test became able to detect 600 copies of the F gene.

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Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

Suarez¹,

Miller²,

Koch³

et al. 2019

Diseases of Poultry

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Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions

Cited by 178 publications

References 24 publications

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

Design, Validation, and Absolute Sensitivity of a Novel Test for the Molecular Detection of Avian Pneumovirus

Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections

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