Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Wang, Zishu; Wang, Jin; Yang, Yan; Bo, Hao; Wang, Rui; Li, Yumei; Wu, Qiong

doi:10.1186/1756-9966-32-76

Cited by 60 publications

(55 citation statements)

References 26 publications

(32 reference statements)

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“…The number of miRNAs with downregulation was more than those with up-regulation, which is in line with most previous miRNA profiling studies in cancer (Guo et al, 2009;Presneau et al, 2013). Three differentially expressed miR-196a (up-regulation), miR27a (up-regulation), and miR-30c (down-regulation) were consistent with previous findings in gastric tumors (Li et al, 2011;Liu et al, 2011;Tsai et al, 2012;Wang et al, 2013). However, we reported here for the first time that miR-193b, miR-214*, solexa-555-1991, miR-502-3p, miR-29c*, miR-64, miR-551b, miR-582-5p, miR-625*, miR-660, miR-363, and miR-486-5p were significantly altered in gastric cancer tissues compared to nontumorous tissues.…”

Section: Discussionsupporting

confidence: 91%

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Tang²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 91%

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Tang²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Reduced production of miR-337 correlates with the lymph node status, although not with age, gender, or tumor position, diameter or differentiation (25,26). However, in the present study, the upregulation of miR-337 indicates that it acts as an oncomiR and may exert an effect on a tumor suppressor.…”

Section: Discussioncontrasting

confidence: 69%

Upregulation of microRNA-337 promotes the proliferation of endometrial carcinoma cells via targeting PTEN

Cai

Liang

et al. 2016

Molecular Medicine Reports

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Abstract. Endometrial carcinoma (EC) is a common malignancy in females. MicroRNAs (miRs) are a class of non-coding RNA that regulate a wide variety of cellular processes, and are important in the development of multiple types of malignancy. In the present study, cancerous and adjacent non-cancerous normal tissue samples were collected from 24 patients diagnosed with EC. Reverse transcription quantitative polymerase chain reaction was performed on the tissue samples to determine the expression levels of six candidate miRs. These miRs have been previously reported to be differentially expressed in EC; however, the present study observed that only miR-337 was differentially expressed. In addition, the current study identified phosphatase and tensin homolog (PTEN) as a target of miR-337 using computational analysis and a luciferase assay. EC cells transfected with miR-337 mimics and anti-PTEN small interfering RNA demonstrated significantly decreased expression of PTEN, markedly increased proliferation and inhibition of cell apoptosis. The results indicate that miR-337 is oncogenic in EC cells, as it suppresses PTEN expression. This may facilitate the development of miR-based prevention or treatment strategies for EC.

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“…Currently, the aberrant expression of many miRNAs has been observed in GC. For example, miR-21, miR-124, miR-125b, miR-221-222 cluster, miR-106b-25 cluster have been shown to contribute to gastric carcinogenesis by changing the cell cycle, cell apoptosis, cell migration and invasion through targeting the relative genes [12,13]. In addition, a lot of miRNAs, especially circulating miRNAs, have been shown to be associated with tumor stages or patient survival, and might be developed as potential biomarkers for GC diagnosis [14].…”

Section: Introductionmentioning

confidence: 99%

The role of microRNA-133b and its target gene FSCN1 in gastric cancer

Guo

Bai

Zou

et al. 2014

J Exp Clin Cancer Res

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Background: Increasing evidences have documented that microRNAs (miRNAs) act as oncogenes or tumor suppressors in gastric cancer (GC). In this study, we aimed to investigate the expression of miR-133b in a large number of GC samples and elucidate its role in GC carcinogenesis and the detailed mechanism. Methods: We used Taqman probe stem-loop real-time PCR to accurately measure the levels of miR-133b in 100 pairs of gastric cancer tissues and the adjacent non-neoplastic tissues. miR-133b mimics were overexpressed in GC cell lines, miR-133b inhibitors were also introduced in GES cells to investigate its role on regulating cell proliferation, cell migration and cell invasion. The target of miR-133b was identified by luciferase reporter assay and western blot. Fascin actin-bundling protein 1 (FSCN1) siRNA was used to achieve the knockdown of FSCN1 in GC cells and to investigate its role on modulating GC cell proliferation and invasion. Results: miR-133b was significantly down-regulated in GC cell lines and in GC tissues compared with adjacent normal tissues. Moreover, lower-level of miR-133b was also associated with venous invasion and a more aggressive tumor phenotype. Re-introduction of miR-133b in GC cells can inhibit cell proliferation, cell migration and invasion. In contrary, knockdown of miR-133b in GES cells can promote cell proliferation and invasion. Further investigation indicated that miR-133b targeted FSCN1 in GC cells and knockdown of FSCN1 can also inhibit GC cell growth and invasion. Conclusion: Our findings demonstrated that miR-133b was significantly down-regulated in GC tissues and exerted its tumor suppressor role in GC cells. The investigation of the detailed mechanism showed that miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in GC cells. These results suggested that miR-133b can be developed as a new diagnostic marker or therapeutic target for GC.

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Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Cited by 60 publications

References 26 publications

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Upregulation of microRNA-337 promotes the proliferation of endometrial carcinoma cells via targeting PTEN

The role of microRNA-133b and its target gene FSCN1 in gastric cancer

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