Loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis

Wardlaw, A. C.; Parton, R.; Hooker, Margaret J.

doi:10.1099/00222615-9-1-89

Cited by 48 publications

(39 citation statements)

References 15 publications

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“…No reversion of these strains to the CR' phenotype has been observed. The concomitant loss of several phase I-related factors along with the Congo red phenotype in these isolates, in strain BP347, in phase-IV strains and under C-mode conditions (Parton and Wardlaw, 1975;Wardlaw et al, 1976), suggests that a common regulatory mechanism is involved. have proposed that a trans-acting positive effector, the product of the vir gene, is responsible for the expression of many of the putative virulence factors of B. pertussis.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media

Parton

1988

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 99%

Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media

Parton

1988

Journal of Medical Microbiology

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“…It is unlikely that the observed 60% inhibition of adenylate cyclase by nicotinic acid fully accounts for this. Nicotinic acid-induced modulation is probably not due to nicotinic acid inhibition of adenylate cyclase activity since nicotinamide, which also caused 60% inhibition of adenylate cyclase activity, does not induce modulation (Wardlaw et a/., 1976). Differences in the amounts of extracellular cAMP in X-and C-mode cultures cannot be attributed to differences in energy charge as intracellular pools of ATP are similar for X-and C-mode cells (Ezzell t't a/., 1981).…”

Section: Discussionmentioning

confidence: 96%

“…C-mode cells were obtained by growing B. pertussis in SS-C medium ( S S -X medium with NaCl replaced by 5 g MgS0,.7H20 I-]). When X-and C-Hornibrook medium was used, this was made up as described previously (Wardlaw et al, 1976). Commercial bovine catalase is a convenient source of adenylate cyclase activator (calmodulin) and this was present in the medium for those experiments w'here stated.…”

Section: Introductionmentioning

confidence: 99%

Adenylate Cyclase Activity During Phenotypic Variation of Bordetella pertussis

Brownlie¹,

Coote²,

Parton³

1985

Microbiology

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“…The two phase-IV cultures that we studied were similar phenotypically to the three cultures in phase I11 but were distinguished retrospectively by their inability to revert to phase I in vivo. Phase-IV variants of B. pertussis are also deficient in a wide range of cellular components and activities by comparison with phase-I strains (Wardlaw et al, 1976), but the mechanisms controlling the loss of such characters are poorly understood. Since this work was completed, Weiss and Falkow (1984) have demonstrated that reversion to phase I can be observed in vitro in B. pertussis and have proposed a model for phase variation.…”

Section: Discussionmentioning

confidence: 99%

Virulence of Bordetella Bronchiseptica in the Porcine Respiratory Tract

Collings

Rutter

1985

Journal of Medical Microbiology

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SUMMARY.The virulence of Bordetella bronchiseptica in gnotobiotic piglets was studied by intranasal infection with 11 cultures derived from eight strains isolated from pigs (4), dogs (2), a human subject and a monkey. Six of the cultures contained organisms in phase I and five contained phenotypically different phase-I11 or -1V organisms. Of the phase-I11 and -1V cultures, four were derived from strains that had been isolated in phase I. Colonisation of the nasal cavity was investigated by counting bacteria in nasal swabs and washings. The toxigenicity of cell extracts from each strain and variant was determined by tests of lethality in mice or of cytopathogenicity in cell cultures. The results showed that two phase-I cultures from pigs colonised the nasal cavity and respiratory tract of gnotobiotic piglets better than did four phase-I cultures from other species. Phase-I organisms invariably produced capsules, fimbriae and mannose-resistant haemagglutination of guinea-pig erythrocytes. Four of five cultures in phases I11 and IV consisted of organisms that did not produce capsules, fimbriae or haemagglutination and colonised the nasal cavity poorly. Phase variation from I to I11 occurred in culture and in vivo, but variation from I11 to I occurred in vivo only and was accompanied by enhanced colonisation. Gnotobiotic piglets infected with porcine phase-I organisms exhibited atrophy of the nasal turbinate bones after 28 days; these organisms produced significantly more toxin than did bacteria in phase I from other species, or those in phases 111 and IV. It was concluded that the development of turbinate atrophy was associated with (1) the ability to produce heavy, persistent colonisation in the nasal cavity, and (2) the production of a heat-labile toxin. Only the two porcine phase-I cultures possessed both properties.

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Loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis

Cited by 48 publications

References 15 publications

Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media

Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media

Adenylate Cyclase Activity During Phenotypic Variation of Bordetella pertussis

Virulence of Bordetella Bronchiseptica in the Porcine Respiratory Tract

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