Low-cost culture medium for the production of proteases by Bacillus mojavensis SA and their potential use for the preparation of antioxidant protein hydrolysate from meat sausage by-products

Hammami, Amal; Bayoudh, Ahmed; Abdelhedi, Ola

doi:10.1007/s13213-018-1352-0

Cited by 28 publications

(11 citation statements)

References 48 publications

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“…Among oligo elements, dipotassium phosphate (1.270 g/L) had the most intense influence on the protease production. This finding is in agreement with Hammami et al (2018) who found that 0.5 g/L of dipotassium phosphate had the greatest effect on the production of alkaline proteases by Bacillus mojavensis.…”

Section: Optimization Of Medium Components By Plackett-burman Designsupporting

confidence: 93%

Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

Akçay

Avcı

2020

Turkish JAF Sci.Tech.

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In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.

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Section: Optimization Of Medium Components By Plackett-burman Designsupporting

confidence: 93%

Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

Akçay

Avcı

2020

Turkish JAF Sci.Tech.

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“…Design of experiment, involving response surface methodology (RSM) and artificial neural network (ANN) methods, has been employed to address the nutritional problems of protease-producing microorganisms, where meeting of significant requirements of microorganisms is prioritized. A major concern during medium development has been cost but exploitation of renewable, especially agro-industrial wastes, as carbon and nitrogen substrates in fermentation media has been most helpful [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila

Asitok

Ekpenyong

Takon

et al. 2022

Biotechnology Reports

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“…Besides, the utilization of by-products for enzyme production is also an environmentally friendly technique because it can reduce the amount of waste generated and released into the environment [23]. Accordingly, this green technique has received a great attention, and as a result, various kinds of enzymes have been effectively produced by using by-products as the major nutrient source such as proteases [22,24], xylanases [21,25], chitosanases [26,27], chitinases [28,29], pectinases [23,30], invertases [31,32], etc.…”

Section: Introductionmentioning

confidence: 99%

Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source

et al. 2021

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Recently, there has been increasing use of agro-byproducts in microbial fermentation to produce a variety of value-added products. In this study, among various kinds of agro-byproducts, pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic enzyme production contained 2% PAP, 0.75% NH4NO3, 0.05% MgSO4, and 0.05% NaH2PO4 and the optimal culture conditions were pH 6.7, 35 °C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were 50 °C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of 1.2 M–1.5 M.

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Low-cost culture medium for the production of proteases by Bacillus mojavensis SA and their potential use for the preparation of antioxidant protein hydrolysate from meat sausage by-products

Cited by 28 publications

References 48 publications

Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila

Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source

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