Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling

Winkler, Thomas; Feil, Michael; Stronkman, Eva Francisca Gerhardine Johanna; Matthiesen, Isabelle; Herland, Anna

doi:10.1039/d0lc00009d

Cited by 37 publications

(53 citation statements)

References 60 publications

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“…Biomaterials Science The cutter plotter is a relatively affordable machine, easy to use and offers a good option for prototyping for microfluidic applications. 40,41,43 The laser cutter is a relatively specialized equipment only available in some facilities.…”

Section: Papermentioning

confidence: 99%

Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

2020

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Section: Papermentioning

confidence: 99%

Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

2020

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“…It is worth noting that these numbers are on the lower end for this type of study (e.g., ~1/3–1/2 compared to our prior work with Caco-2- or primary BBB-on-chip). [6,50] We attribute this to the relatively minimal, protein-poor media we employ here.…”

Section: Resultsmentioning

confidence: 99%

“…For metabolomics, we followed two parallel workflows, aimed either at annotated compounds using ThermoFisher’s proprietary software, [78] or at network-level results using open-source tools. [50] Since both SIN-1 and NACA acted not only on the cells, but also reacted with the media itself, we employed media background-subtraction in our workflows for more meaningful comparisons. For metabolomics data only, we conducted statistical analyses in R and (by design) needed to rely on p -values.…”

Section: Methodsmentioning

confidence: 99%

Continuous monitoring reveals protective effects ofN-acetylcysteine amide on an isogenic microphysiological model of the neurovascular unit

Matthiesen

Voulgaris

Nikolakopoulou

et al. 2021

Preprint

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Microphysiological systems mimic the in vivo cellular ensemble and microenvironment with the goal of providing more human-like models for biopharmaceutical research. We report the first such model of the blood-brain barrier (BBB-on-chip) featuring both isogenic human induced pluripotent stem cell (hiPSC)-derived cells and continuous barrier integrity monitoring with <2-minute temporal resolution. We showcase its capabilities in the first microphysiological study of nitrosative stress and antioxidant prophylaxis. Relying on off-stoichiometry thiol-ene epoxy (OSTE+) for fabrication greatly facilitates assembly and sensor integration compared to the prevalent polydimethylsiloxane devices. The integrated cell-substrate endothelial resistance monitoring allows us to capture formation and breakdown of our blood-brain barrier model, consisting of co-cultured hiPSC-derived endothelial-like and astrocyte-like cells. We observe clear cellular disruption when exposing the BBB-on-chip to the nitrosative stressor linsidomine, and report on the barrier permeability and barrier-protective effects of the antioxidant N-acetylcysteine amide. Using metabolomic network analysis, we further find drug-induced changes consistent with prior literature regarding, e.g., cysteine and glutathione involvement. A model like ours opens new possibilities for drug screening studies and personalized medicine, relying solely on isogenic human-derived cells and providing high-resolution temporal readouts that can help in pharmacodynamic studies.

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“…The feasibility and success of integration are dependent on advancements in microengineering and microfabrication technologies. Methods like plasma-activated bonding, 813 anodic bonding, 814 thin glue layer deposition by microstamping, 815 and microchannel fabrication in a double-sided tape 816 are some of the recent developments that enabled the robust assembly of microfluidic compartments onto various substrates with 2D and 3D gradients.…”

Section: Microfluidic Technologies For Biomaterials Discovery and Biointerface Understandingmentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling

Abstract: High costs are a key challenge in “democratization” of organ-chip research. We present a low-resource barrier-on-chip based on tape, and use it to model small intestine and its response to chili peppers (capsaicinoids).

Cited by 37 publications

References 60 publications

Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

Continuous monitoring reveals protective effects ofN-acetylcysteine amide on an isogenic microphysiological model of the neurovascular unit

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

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