Low-dose exposure to phytosynthesized gold nanoparticles combined with glutamine deprivation enhances cell death in the cancer cell line HeLa <i>via</i> oxidative stress-mediated mitochondrial dysfunction and G0/G1 cell cycle arrest

Prasad, Abhinav; Khatua, Ashapurna; Mohanta, Yugal Kishore; Saravanan, Muthupandian; Meena, Ramovatar; Ghosh, Indraneel

doi:10.1039/d2nr02150a

Cited by 30 publications

(11 citation statements)

References 83 publications

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“…This happens due to the production of ROS induced by AgNPs which inhibits ATP synthesis and subsequently damaging the DNA [ 59 ]. Oxidative stress caused by NPs have been reported to induces mitochondrial dysfunction which suppress the division of cancerous cells [ 60 ] In this assay, cells were treated with the synthesized AgNPs (200 μg/ml) for 24hrs. In cytotoxicity of HepG2 cell lines by AgNPs (200 μg/ml) exhibited strong activity by S-AgNPs (83.82%), followed by RS-AgNPs (74.55%) and R-AgNPs (44.82%) ( Fig 8B, 8C ).…”

Section: Resultsmentioning

confidence: 99%

Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

Rehman

Ali

et al. 2023

PLoS ONE

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Green synthesis of nanoparticles is becoming a method of choice for biological research due to its environmentally benign outcomes, stability and ease of synthesis. In this study, silver nanoparticles (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) and mixture of stem and root (RS-AgNPs) of Delphinium uncinatum. The synthesized nanoparticles were characterized by standardized techniques and evaluated for their antioxidant, enzyme inhibition, cytotoxic and antimicrobial potentials. The AgNPs exhibited efficient antioxidant activities and considerable enzyme inhibition potential against alpha amylase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. S-AgNPs showed strong cytotoxicity against human hepato-cellular carcinoma cells (HepG2) and high enzyme inhibitory effect (IC50 values 27.5μg/ml for AChE and 22.60 μg/ml for BChE) compared to R-AgNPs and RS-AgNPs. RS-AgNPs showed significant inhibition of Klebsiella pneumoniae and Aspergillus flavus and exhibited higher biocompatibility (<2% hemolysis) in human red blood cells hemolytic assays. The present study showed that biologically synthesized AgNPs using the extract of various parts of D. uncinatum have strong antioxidant and cytotoxic potentials.

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Section: Resultsmentioning

confidence: 99%

Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

Rehman

Ali

et al. 2023

PLoS ONE

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“…The cells were seeded into 96-well plates at a density of 5000–10,000 cells per well. After 24 h, cells were washed with PBS buffer and treated with 100 μM DCFH-DA for 1 h [ 52 ]. Cells were again washed with PBS and treated with the IC 50 concentration of PTAgNPs and untreated wells were considered as the control.…”

Section: Methodsmentioning

confidence: 99%

Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach

Alghuthaymi

Patil²,

Rajkuberan

et al. 2023

Plants

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Plant-mediated metallic nanoparticles have beenreported for a diversified range of applications in biological sciences. In the present study, we propose the Polianthes tuberosa flower as a reducing and stabilizing agent for the synthesis of silver nanoparticles (PTAgNPs). The PTAgNPs were exclusively characterized using UV–Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy, zeta potential, and transmission electron microscopy (TEM) studies. In a biological assay, we investigated the antibacterial and anticancer activity of silver nanoparticles in the A431 cell line. The PTAgNPs demonstrated a dose-dependent activity in E. coli and S. aureus, suggesting the bactericidal nature of AgNPs. The PTAgNPs exhibited dose-dependent toxicity in the A431 cell line, with an IC50 of 54.56 µg/mL arresting cell growth at the S phase, as revealed by flow cytometry analysis. The COMET assay revealed 39.9% and 18.15 severities of DNA damage and tail length in the treated cell line, respectively. Fluorescence staining studies indicate that PTAgNPs cause reactive oxygen species (ROS) and trigger apoptosis. This research demonstrates that synthesized silver nanoparticles have a significant effect on inhibiting the growth of melanoma cells and other forms of skin cancer. The results show that these particles can cause apoptosis or cell death in malignant tumor cells. This suggests that they could be used to treat skin cancers without harming normal tissues.

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“…[80,81] Therefore, 1 mM AgNO 3 concentration was accepted as the optimum concentration for the biosynthesis of small-size HP-AgNPs and corroborating the results of Azmi et al [82] Silver nanoparticle synthesis is a pH-dependent reaction by altering the electrical load of phytochemicals and ultimately influences the capping and stabilizing capacity of Ag ions. [83] In the current work, the pH 5, 6, 7, 8, 9, 10, 11, and 12 were chosen for the optimization of HP-AgNPs fabrication. The results suggested that no UV absorption peaks appeared at lower pH (5 and 6), showing that an acidic medium is not appropriate for NPs synthesis.…”

Section: Discussionmentioning

confidence: 99%

Fabrication and characterization of Hypecoum pendulum‐assisted novel bio‐inspired silver nanoparticles and their antioxidant, anti‐diabetic, and anti‐Alzheimer properties

Huda

Ahmed

Mushtaq

et al. 2023

Applied Organom Chemis

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This research aims to study the biogenic synthesis and characterization of Hypecoum pendulum extract-induced silver nanoparticles (HP-AgNPs) and to investigate its antioxidant, anti-alpha-amylase, and anti-acetylcholinesterase (AChE) activities. UV spectrometry at 417 nm with an surface plasmon resonance (SPR) peak of 1.54 confirmed the biosynthesis of HP-AgNPs. X-ray diffraction (XRD) analysis demonstrated that HP-AgNPs are crystalline with a 36 nm size. The average size of 80 nm was confirmed by scanning electron microscopy (SEM) analysis. The functional moieties bearing compounds accountable for the encapsulation and stability of HP-AgNPs were assessed by the Fourier transform infrared (FTIR) technique. Energy dispersive X-ray (EDX) demonstrated that silver was the main element (49%) of HP-AgNPs. HP-AgNPs showed the promising antioxidant potential of ABTS (71.57%), diphenyl-1-picrylhydrazyl (DPPH, 69.46%), H 2 O 2 (66.94%), ferric chloride (73.71%), and ammonium molybdenum (65.7%). HP-AgNPs exhibited significant enzymatic inhibition potential with IC 50 of 48.8 ± 4.03 μg/mL (α-amylase) and 51.78 ± 3.16 μg/mL (AChE). Kinetics studies showed that HP-AgNPs produced mixed-type inhibition against α-amylase (K m increased from 40.65 to 131.44%, whereas V max decreased from 4.14 to 9.77%) and non-competitivetype inhibition (V max decreased from 17.05 to 62.03% and K m remain constant) against AChE. Finally, it may be concluded that HP-AgNPs may possess anti-Alzheimer, anti-diabetic, and antioxidant capabilities.

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Low-dose exposure to phytosynthesized gold nanoparticles combined with glutamine deprivation enhances cell death in the cancer cell line HeLa via oxidative stress-mediated mitochondrial dysfunction and G0/G1 cell cycle arrest

Abstract: Cancer cells use nutrients like D-glucose (Glc) and L-glutamine (Q) more efficiently for their development. This increased nutritional dependency of malignant cells has been commonly employed in various in vitro...

Cited by 30 publications

References 83 publications

Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach

Fabrication and characterization of Hypecoum pendulum‐assisted novel bio‐inspired silver nanoparticles and their antioxidant, anti‐diabetic, and anti‐Alzheimer properties

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