Low-energy formation of edible nanoemulsions: Factors influencing droplet size produced by emulsion phase inversion

Ostertag, Felix; Weiß, Jochen; McClements, David Julian

doi:10.1016/j.jcis.2012.07.089

Cited by 347 publications

(244 citation statements)

References 38 publications

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“…This was the result of using a suitable surfactant pair, composed of soy phosphatidylcholine and sodium oleate (1:7 w/w) associated with the co-solvent 2-methylpyrrolidone (2% w/v). The effect of surfactant composition [5] and co-solvent ratio [15] on both droplet size and droplet size distribution has been previously described. In addition, the composition of oil phase/surfactant/co-solvent used in this work produced stable nanoemulsions with high negative ZPs, guaranteeing suitable electrostatic resilience against flocculation.…”

Section: Resultsmentioning

confidence: 99%

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Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Islam

Streck²,

Paz³

et al. 2016

Int Arch Med

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The study prepared a nanoemulsion with a diterpenoid isoprenoid alcohol called phytol (PYT) and subsequently tested it for antioxidant capacity. For this, PYT-loaded nanoemulsion was prepared by phase inversion method and both PYT-containing nanoemulsion (PNE) and PYT-free nanoemulsion (PFNE) (2-16 µM) were tested for antiradical activity (DPPH•: 1,1-dipheny-picrylhydrazyl radical; ABTS•+: azino-bisethylbenzthiazoline-sulfonic acid; •OH: hydroxyl radical scavenging; NO•: nitrite oxide radical), lipid peroxidation (LP), reduction potential (RP), and inhibition of hemolysis (HL) in rat erythrocytes in comparison with an α-tocopherol analogue (Trolox -TRO -positive control). In addition, an in vivo test was performed with wildtype and deficient Saccharomyces cerevisiae strains using hydrogen peroxide (H 2 O 2 ) as a stressor. Results suggest that PNE exhibited higher antioxidant than the PFNE. Increasing doses reveled antioxidant capacity in a dose-dependent manner. In the S. cerevisiae study, both PFNEand PNE-treated groups exhibited decreased rates of survival with the highest doses, whichever in the presence of stressor increased the survival rates, which indicates antioxidative defense capacity of PYT. In this occasion, PNE exhibited prominent antioxidative defense in the presence of stressor rather than PFNE. In conclusion, PYT exhibited potential antioxidant activity but at high concentration it was toxic to the yeast cells. The production of PYT-nanoemulsions may be relevant to the pharmaceutical sciences.

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Section: Resultsmentioning

confidence: 99%

“…This was emulsified using a basic Ultra-Turrax T18 equipped with the S 18 N-19 G dispersing tool at 11,000 rpm for one minute followed by four minutes at 7,000 rpm. The surfactant mixture that produced transparent colloidal dispersions was selected in a previous study using a pseudo-ternary phase diagram [5].…”

Section: Preparation Of Pnementioning

confidence: 99%

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Islam

Streck²,

Paz³

et al. 2016

Int Arch Med

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“…At this point, hydrophilic-lipophilic properties are balanced and further addition of water not only changes the surfactant curvature from zero to positive but also transforms w/o microemulsions into o/w nanoemulsions 22,23 . The preparation of nanoemulsions by PIC method can broadly be divided into 3 steps: mixing of the organic phase (oil + surfactant), titration of aqueous phase into an organic phase, and additional mixing 28,38,39 .…”

Section: Phase Inversion Composition (Pic)mentioning

confidence: 99%

Preparation of nanoemulsions by phase inversion temperature (PIT)

Jintapattanakit¹

2018

Pharm Sci Asia

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“…In this regard, nanoemulsions are very attractive colloidal systems for therapeutic and nutritional oils (Ostertag et al 2012). By definition, nanoemulsions are kinetically stable emulsion systems, where oil droplets are dispersed in an external aqueous phase, with a thin layer of emulsifier as stabilizing agent (Bouchemal et al 2004).…”

Section: Research Articlementioning

confidence: 99%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

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(2016) Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells, Pharmaceutical Biology, 54:12, 2968-2977, DOI: 10.1080/13880209.2016 Context: Glioma is a malignant brain tumor with rapid proliferation, infiltrative growth, poor prognosis and it is chemoresistent. Pomegranate seed oil (PSO) has antioxidant, anti-inflammatory and antitumor properties. This study showed the optimization of PSO nanoemulsions (NEs) as an alternative for glioma treatment.Objective: The study aimed to evaluate PSO NEs cytotoxicity on human blood cells and antiglioma effects against C6 cells. Materials and methods: NEs were prepared by the spontaneous emulsification method, using PSO at 1.5 and 3.0%, and were evaluated regarding their physical stability and antioxidant activity. Toxicity evaluations in human blood cells were performed in terms of cell viability, genotoxicity, lipid peroxidation, protein carbonylation, catalase activity and hemolysis at 0.1, 0.25 and 0.5 mg/mL PSO, after a 72-h incubation period. In vitro antitumor effect was determined against glioma cells after 24 and 48 h, and astrocytes were used as a non-transformed cell model. Results: Formulations presented droplet size below 250 nm, low polydispersity index, negative zeta potential and pH in the acid range. NEs and PSO had scavenging capacity around 30% and promoted a proliferative effect in mononuclear cells, increasing about 50% cell viability. No genotoxic and oxidative damage was observed in lipid peroxidation, protein carbonylation and catalase activity evaluations for NEs. Hemolysis study showed a hemolytic effect at high concentrations. Moreover, formulations reduced only tumor cell viability to 47%, approximately. Discussion and conclusion: Formulations are adequate and safe for intravenous administration. Besides, in vitro antitumor activity indicates that NEs are promising for glioma treatment.ARTICLE HISTORY

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Low-energy formation of edible nanoemulsions: Factors influencing droplet size produced by emulsion phase inversion

Cited by 347 publications

References 38 publications

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Preparation of phytol-loaded nanoemulsion and screening for antioxidant capacity

Preparation of nanoemulsions by phase inversion temperature (PIT)

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

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