Low-Energy Laser Irradiation Stimulates Bone Nodule Formation at Early Stages of Cell Culture in Rat Calvarial Cells

Ozawa, Yasuhiro; Shimizu, Nobutaka; Kariya, Genichiro; Abiko, Yoshimitsu

doi:10.1016/s8756-3282(97)00294-9

Cited by 305 publications

(232 citation statements)

References 33 publications

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“…Laser therapy is a new approach in different medical fields, including orthopedics and dentistry, when bone loss occurs, that is, in cases of bone fracture and tooth extraction [1]. Recent studies have reported the benefits of low level laser therapy (LLLT), which has been used to induce softtissue healing, for pain relief, bone, and nerve regeneration [2], although the molecular mechanisms triggered are not yet fully clear.…”

Section: Introductionmentioning

confidence: 99%

Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors

et al. 2009

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Background and Objective: Laser therapy is a new approach applicable in different medical fields when bone loss occurs, including orthopedics and dentistry. It has also been used to induce soft-tissue healing, for pain relief, bone, and nerve regeneration. With regard to bone synthesis, laser exposure has been shown to increase osteoblast activity and decrease osteoclast number, by inducing alkaline phosphatase (ALP), osteopontin, and bone sialoprotein expression. Studies have investigated the effects of continuous or pulsed laser irradiation, but no data are yet available on the properties of superpulsed laser irradiation. This study thus aimed to investigate the effect of superpulsed laser irradiation on osteogenic activity of human osteoblast-like cells, paying particular attention to investigating the molecular mechanisms underlying the effects of this type of laser radiation. Study Design/Materials and Methods: Human osteoblast-like MG-63 cells were exposed to 3, 7, or 10 superpulsed laser irradiation (pulse width 200 nanoseconds, minimum peak power 45 W, frequency 30 kHz, total energy 60 J, exposure time 5 minutes). The following parameters were evaluated: cell growth and viability (light microscopy, lactate dehydrogenase release), calcium deposits (Alizarin Red S staining), expression of bone morphogenetic factors (real-time PCR). Results: Superpulsed laser irradiation decreases cell growth, induces expression of TGF-b2, BMP-4, and BMP-7, type I collagen, ALP, and osteocalcin, and increases the size and the number of calcium deposits. The stimulatory effect is maximum on day 10, that is, after seven applications. Conclusions: Reported results show that superpulsed laser irradiation, like the continuous and pulsed counterparts, possesses osteogenic properties, inducing the expression of molecules known to be important mediators of bone formation and, as a consequence, increasing calcium deposits in human MG-63 cells. Moreover, the data suggest a new potential role for PPARg as a regulator of osteoblast proliferation.

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Section: Introductionmentioning

confidence: 99%

Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors

et al. 2009

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“…It is considered a noninvasive, safe technique to stimulate osteogenesis [23,24]. Several previous studies have shown that LLLT could increase both osteoblast and osteoclast activities, together with stimulating the formation of callus and new bone matrix [25][26][27][28]. Of note, LLLT alone as a treatment modality in surgical defects enhanced faster and a greater area of bone formation compared to the PRF treatment modality.…”

Section: Discussionmentioning

confidence: 99%

Usage of Low Level Laser Biostimulation and Platelet Rich Fibrin in Bone Healing: Experimental Study

Elhayes¹,

Zaky²,

Ibrahim³

et al. 2016

Dent. Med. Probl.

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Low level laser therapy (LLLT) is defined as supplying direct biostimulative light energy to the cells. The wound healing process could be enhanced using low-level semiconductor diode lasers [1]. It has been reported that absorbed laser energy stimulates the molecules and atoms of the body's cells [2].While several studies have demonstrated that LLLT has stimulating effects on stem cells of the AbstractBackground. Experimental studies have shown that low level laser therapy (LLLT) has a positive local biostimulative effect in the early stage of bone healing. Platelet rich fibrin (PRF) also has been shown to be effective in the treatment of intrabony periodontal defects. Objectives. The objective of our experimental study was to demonstrate the combined effects of LLLT and PRF on bone healing. Material and Methods. Our experimental study was done over 80 bony cavities in 20 adult male rabbits, aged 12 months. An incision was made for exposure of the femur bone of all rabbits. Then, by using a large, round surgical bur, a perforated hole was made in the femur. The cavities induced in these rabbits were divided into 4 groups: The control group which was neither subjected to any laser irradiation nor filled with any bone substitute (group I); The bony defects were filled with PRF (group II); The cavities were subjected to low level laser (LLL) for biostimulation (group III); The cavities were subjected to LLL for biostimulation then were filled with PRF (group IV). Histological assessments of the four groups were done using a hematoxylin and eosin stain. Statistical analysis was done using ANOVA and Bonferroni tests for comparisons between the four groups.Results. The area percentage of the newly formed bone in group IV was significantly higher than the other three groups. The area percentage of the newly formed bone in group III is significantly higher than group II. Conclusions. LLLT could induce bone formation in the bone defect at a faster rate than PRF. However, a combination of both LLLT and PRF as treatment modalities could induce bone formation in the bone defect more than that of LLLT or PRF alone (Dent. Med. Probl. 2016, 53, 3, 338-344).

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“…Oliveira et al [6] showed that neither the MTT values nor mRNA expression of collagen I in the irradiated group differed significantly from those in the non-irradiated odontoblast-like cells. On the other hand, Ozawa et al [7] reported that laser irradiation at an earlier stage of bone formation was more effective than irradiation at a later stage, and that stimulation of bone formation by laser was dependent on the total energy dose. Therefore, it's important to investigate the proliferation effect of 638 nm red laser light on bone marrow MSCs with or without osteogenic supplements.…”

Section: Introductionmentioning

confidence: 99%

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

Peng¹

2016

OPJ

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Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin, nerve, and skeletal muscle tissues and bone. Bone Mesenchymal stem cells (BMSCs)are derived from bone marrow, which exhibited a fibroblast-like appearance, and could differentiate in vitro into different lineages. However, there is a reciprocal relationship between growth and osteogenic differentiation in MSCs. Therefore, it's important to investigate the effect of LLLI on BMSCs. The aim of our study was to investigate the proliferation effect of 635 nm red laser light on bone marrow MSCs with or without osteogenic supplements. Bone marrow was collected from the 4-week-old Sprague-Dawley rats femur and tibiae. MSCs with and without osteogenic supplements both were divided into three groups. A continuous 635 nm wavelength red light diode laser (a power output of 960 mW) was used in the study. The size of light spot was 35mm in diameter. Irradiation was performed every other day since the half of medium was changed to osteogenic differentiation media (ODM). The first irradiation day was set as 0 day. The duration of each irradiation for red light was calculated at 10 seconds for 1 J/cm 2 , 20 seconds for 2 J/cm 2 . Two of these groups were used as controls: MSCs incubated in DMEM without irradiation (control 1), MSCs incubated in ODM without irradiation (control 2). Cellular proliferation was evaluated by using WST-8. Cell viability was assessed with WST-8 kit at 2, 4, 6 and 8 days, respectively. At 4, 6 and 8 days, groups cultured with DMEM showed significantly higher viabilities than that in groups with ODM. In groups with DMEM, red light at all doses significantly stimulated cell viability as compared with the control 1. Groups irradiated at 1 and 2 J/cm 2 had more effective proliferation on 4 (P < 0.01) and 6 days (P < 0.05), when compared with the control 1. In groups with ODM, control 2 and the irradiated groups showed similar proliferation speeds. In conclusion, we can find that red light can promote proliferation of MSCs cultured in normal media, and suppress proliferation of MSCs cultured in ODM.

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Low-Energy Laser Irradiation Stimulates Bone Nodule Formation at Early Stages of Cell Culture in Rat Calvarial Cells

Cited by 305 publications

References 33 publications

Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors

Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors

Usage of Low Level Laser Biostimulation and Platelet Rich Fibrin in Bone Healing: Experimental Study

The Effect of 635 nm Red Laser Irradiation on Proliferation of Bone Marrow Stem Cells

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