Low shear stress induces endothelial reactive oxygen species via the AT1R/eNOS/NO pathway

Chao, Yuelin; Ye, Peng; Zhu, Liping; Kong, Xiangquan; Qu, Xinliang; Zhang, Junxia; Luo, Jie; Yang, Hongfeng; Chen, Shao‐Liang

doi:10.1002/jcp.26016

Cited by 41 publications

(29 citation statements)

References 38 publications

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“…LSS can also cause oxidative stress and promote the occurrence of AS [25]. In the present study, we found that LSS significantly reduced the activity of SOD and GSH and significantly increased the levels of ROS, MDA, and GSSG, breaking the "oxidation-reduction" balance of EC.…”

supporting

confidence: 58%

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Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Fan

Han

Zhu

et al. 2020

Oxidative Medicine and Cellular Longevity

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Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention.

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supporting

confidence: 58%

“…EA.hy 926 cells were exposed to laminar flow with a value of 0 or 3 dyn/cm 2 for 30 min [ 25 ]. LSS significantly reduced the release of NO and PGI 2 and significantly increased the release of ET-1, which was significantly inhibited by 1 mg/L and 10 mg/L DHP, but not by 0.1 mg/L DHP (Figures 4(a) – 4(c) ).…”

Section: Resultsmentioning

confidence: 99%

Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Fan

Han

Zhu

et al. 2020

Oxidative Medicine and Cellular Longevity

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show abstract

“…In these cases, control experiments under normoxia/normocapnia could be carried out to evaluate the sole effects of shear stress, but care should be taken in the interpretation, since the possible effects of a double-hit (cyclic hypoxia/hypercapnia plus simultaneous shear stress) challenge effect should not be discarded. In fact, it has recently been found that low shear stress induces endothelial reactive oxygen species (Chao et al, 2018), thereby potentially contributing to the oxidative stress response induced by intermittent hypoxia/hyperoxia.…”

Section: Optimized Control Of Gas Partial Pressure In Cultured Cellsmentioning

confidence: 99%

Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches

et al. 2018

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Gas partial pressures within the cell microenvironment are one of the key modulators of cell pathophysiology. Indeed, respiratory gases (O2 and CO2) are usually altered in respiratory diseases and gasotransmitters (CO, NO, H2S) have been proposed as potential therapeutic agents. Investigating the pathophysiology of respiratory diseases in vitro mandates that cultured cells are subjected to gas partial pressures similar to those experienced by each cell type in its native microenvironment. For instance, O2 partial pressures range from ∼13% in the arterial endothelium to values as low as 2–5% in cells of other healthy tissues and to less than 1% in solid tumor cells, clearly much lower values than those used in conventional cell culture research settings (∼19%). Moreover, actual cell O2 partial pressure in vivo changes with time, at considerably different timescales as illustrated by tumors, sleep apnea, or mechanical ventilation. Unfortunately, the conventional approach to modify gas concentrations at the above culture medium precludes the tight and exact control of intra-cellular gas levels to realistically mimic the natural cell microenvironment. Interestingly, well-controlled cellular application of gas partial pressures is currently possible through commercially available silicone-like material (PDMS) membranes, which are biocompatible and have a high permeability to gases. Cells are seeded on one side of the membrane and tailored gas concentrations are circulated on the other side of the membrane. Using thin membranes (50–100 μm) the value of gas concentration is instantaneously (<0.5 s) transmitted to the cell microenvironment. As PDMS is transparent, cells can be concurrently observed by conventional or advanced microscopy. This procedure can be implemented in specific-purpose microfluidic devices and in settings that do not require expensive or complex technologies, thus making the procedure readily implementable in any cell biology laboratory. This review describes the gas composition requirements for a cell culture in respiratory research, the limitations of current experimental settings, and also suggests new approaches to better control gas partial pressures in a cell culture.

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“…The glycocalyx is composed of many kinds of molecules aside from HA, including heparan sulfate, chondroitin sulfate, and sialic acid, as well as a glycocalyx layer that may not be regulated by the AMPK, NHE1, and HYAL2 axis but is also damaged by LSS. Moreover, we previously reported increased ROS, which degrades HA directly, in HUVECs in response to LSS via the angiotensin II type 1 receptor pathway (34,35). This finding implies that other signaling pathways may participate in LSS-induced glycocalyx impairment.…”

Section: Discussionmentioning

confidence: 80%

AMP‐activated protein kinase regulates glycocalyx impairment and macrophage recruitment in response to low shear stress

et al. 2019

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Low shear stress (LSS) increases degradation of the endothelial glycocalyx, leading to production of endothelial inflammation and atherosclerosis. However, the underlying mechanisms of how LSS diminishes the endothelial glycocalyx remain unclear. We showed that LSS inactivated AMPK, enhanced Na+‐H+ exchanger (NHE)1 activity, and induced glycocalyx degradation. Activation of AMPK prevented LSS‐induced NHE1 activity and endothelial glycocalyx impairment. We further identified hyaluronidase 2 (HYAL2) as a mediator of endothelial glycocalyx impairment in HUVECs exposed to LSS. Inactivation of AMPK by LSS up‐regulates the activity of HYAL2, which acts downstream of NHE1. We characterized a left common carotid artery partial ligation (PL) model of LSS in C57BL/6 mice. The results showed decreased expression of hyaluronan (HA) in the endothelial glycocalyx and decreased thickness of the endothelial glycocalyx in PL mice. Pharmacological activation of AMPK by ampkinone not only attenuated glycocalyx impairment due to HA degradation but also blocked vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 expression increase and macrophage recruitment in the endothelia of PL mice. Our results revealed that AMPK dephosphorylation induced by LSS activates NHE1 and HYAL2 to promote HA degradation and glycocalyx injury, which may contribute to endothelial inflammatory reaction and macrophage recruitment.—Zhang, J., Kong, X., Wang, Z., Gao, X., Ge, Z., Gu, Y., Ye, P., Chao, Y., Zhu, L., Li, X., Chen, S. AMP‐activated protein kinase regulates glycocalyx impairment and macrophage recruitment in response to low shear stress. FASEB J. 33, 7202–7212 (2019). http://www.fasebj.org

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Low shear stress induces endothelial reactive oxygen species via the AT1R/eNOS/NO pathway

Cited by 41 publications

References 38 publications

Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches

AMP‐activated protein kinase regulates glycocalyx impairment and macrophage recruitment in response to low shear stress

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