Protective Activities of <i>Dendrobium huoshanense</i> C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Fan, Xiangcheng; Han, Jichun; Zhu, Lijun; Chen, Zhipeng; Li, Jiajing; Gu, Yue; Wang, Rui; Wang, Tao; Yue, Yunyun; Shang, Jing

doi:10.1155/2020/8365056

Cited by 19 publications

(15 citation statements)

References 35 publications

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“…Referring to our previous research [ 26 ], EA.hy926 cells were exposed to laminar flow with a value of 0 or 3 dyn/cm 2 for 30 min. LSS significantly reduced the release of NO and PGI 2 and significantly increased the release of ET-1, which was significantly inhibited by 1 mg/L and 10 mg/L DHWE but not by 0.1 mg/L DHWE (Figures 7(a) – 7(c) ).…”

Section: Resultsmentioning

confidence: 99%

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Dendrobium catenatum Lindl. Water Extracts Attenuate Atherosclerosis

Han

Dong

Zhang

et al. 2021

Mediators of Inflammation

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Objectives. Dendrobium catenatum Lindl. (DH) is a Chinese herbal medicine, which is often used to make tea to improve immunity in China. Rumor has it that DH has a protective effect against cardiovascular disease. However, it is not clear how DH can prevent cardiovascular disease, such as atherosclerosis (AS). Therefore, the purpose of this study is to study whether DH can prevent AS and the underlying mechanisms. Methods. Zebrafish larvae were fed with high-cholesterol diet (HCD) to establish a zebrafish AS model. Then, we used DH water extracts (DHWE) to pretreat AS zebrafish. The plaque formation was detected by HE, EVG, and oil red O staining. Neutrophil and macrophage counts were calculated to evaluate the inflammation level. Reactive oxygen species (ROS) activity, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity in zebrafish were measured to reflect oxidative stress. The cholesterol accumulation and the levels of lipid, triglyceride (TG), and total cholesterol (TC) were measured to reflect lipid metabolism disorder. Then, parallel flow chamber was utilized to establish a low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. EA.hy926 cells were exposed to LSS (3 dyn/cm2) for 30 min and treated with DHWE. The levels of ROS, SOD, MDA, glutathione (GSH), and glutathiol (GSSG) in EA.hy926 cells were analysed to determine oxidative stress. The release of nitric oxide (NO), endothelin-1 (ET-1), and epoprostenol (PGI2) in EA.hy926 cells was measured to reflect EC dysfunction. The mRNA expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in EA.hy926 cells was detected to reflect EC dysfunction inflammation. Results. The results showed that DHWE significantly reduced cholesterol accumulation and macrophage infiltration in early AS. Finally, DHWE significantly alleviate the lipid metabolism disorder, oxidative stress, and inflammation to reduce the plaque formation of AS zebrafish larval model. Meanwhile, we also found that DHWE significantly improved LSS-induced EC dysfunction and oxidative stress in vitro. Conclusion. Our results indicate that DHWE could be used as a prevention method to prevent AS.

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Section: Resultsmentioning

confidence: 99%

“…LSS can also cause oxidative stress and promote the occurrence of AS [ 26 ]. In the present study, we found that LSS significantly reduced the activity of SOD and GSH and significantly increased the levels of ROS, MDA, and GSSG, breaking the “oxidation-reduction” balance of ECs.…”

Section: Discussionmentioning

confidence: 99%

Dendrobium catenatum Lindl. Water Extracts Attenuate Atherosclerosis

Han

Dong

Zhang

et al. 2021

Mediators of Inflammation

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“…Studies have found that zebrafish can be used for researches in the early AS [ 17 ]. After feeding zebrafish HCD for 10 days, there will be cholesterol accumulation in the blood vessels similar to those in patients with early AS, but no plaques were observed [ 10 , 16 ]. In the present study, we fed zebrafish HCD to establish an HCD-induced AS (HCD-AS) model.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, zebrafish are invaluable for studying the development and physiology of vertebrate as well as modeling human diseases. Our previous studies have shown that a zebrafish hypercholesterolemia model induced by a high-cholesterol diet can be applied to reflect the early symptoms of human AS that cholesterol accumulation in blood vessels can be observed in vivo [ 10 ]. However, there was no hallmark phenomenon of plaque appears.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish Model for Screening Antiatherosclerosis Drugs

Han

Zhang

et al. 2021

Oxidative Medicine and Cellular Longevity

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This study is aimed at establishing a zebrafish model of AS, which can be applied for high-throughput screening anti-AS drugs. A zebrafish AS model was induced by high cholesterol diet (HCD) and lipopolysaccharide (LPS). In the early stage of modeling, HCD induced zebrafish to show some early symptoms similar to human AS, mainly cholesterol accumulation, vascular inflammation, lipid metabolism disorder, and oxidative stress. In addition to lipid metabolism disorders, LPS also induced the same symptoms. And when HCD and LPS exist at the same time, these AS symptoms in zebrafish become more severe. When the modeling time reached 45 days, HCD and LPS induce the formation of plaques in zebrafish blood vessels, and these plaques contain fibrous tissue and lipids, which are similar to human AS plaques. We also evaluated the efficacy of some anti-AS drugs (atorvastatin, aspirin, and vitamin C) through these zebrafish AS models. The results found that atorvastatin can significantly reduce the symptoms of AS induced by HCD and LPS, and aspirin and vitamins can significantly reduce the symptoms of AS induced by LPS. It is feasible to use zebrafish to establish an AS model, and the zebrafish AS model can be used for high-throughput screening of anti-AS drugs.

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“…The leaves of D. huoshanense have long been utilized for dermatologic disorders, metabolic syndromes, nervous system disorders, and musculoskeletal system disorders ( Wang, 2021 ). Modern pharmacological research has revealed that D. huoshanense has anti-inflammatory ( Ge et al, 2018 ; Li et al, 2020 ), cytotoxic ( Chen et al, 2022 ), hypoglycemic ( Wang et al, 2019 ), anti-atherosclerosis ( Fan et al, 2020 ), and antioxidant ( Tian et al, 2013 ) activity. Dendrobium plant is well-known for its rich and diverse endophytic bacterial and fungal community ( Chen et al, 2019 ; Chen et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Huoshanmycins A‒C, New Polyketide Dimers Produced by Endophytic Streptomyces sp. HS-3-L-1 From Dendrobium huoshanense

Zhu

Kong

et al. 2022

Front. Chem.

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Three new polyketide dimers named huoshanmycins A‒C (1–3) were isolated from a plant endophytic Streptomyces sp. HS-3-L-1 in the leaf of Dendrobium huoshanense, which was collected from the Cultivation base in Jiuxianzun Huoshanshihu Co., Ltd. The dimeric structures of huoshanmycins were composed of unusual polyketides SEK43, SEK15, or UWM4, with a unique methylene linkage. Their structures were elucidated through comprehensive 1D-/2D-NMR and HRESIMS spectroscopic data analysis. The cytotoxicity against MV4-11 human leukemia cell by the Cell Counting Kit-8 (CCK8) method was evaluated using isolated compounds with triptolide as positive control (IC50: 1.1 ± 0.4 μM). Huoshanmycins A and B (1, 2) displayed moderate cytotoxicity with IC50 values of 32.9 ± 7.2 and 33.2 ± 6.1 μM, respectively.

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Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Cited by 19 publications

References 35 publications

Dendrobium catenatum Lindl. Water Extracts Attenuate Atherosclerosis

Dendrobium catenatum Lindl. Water Extracts Attenuate Atherosclerosis

Zebrafish Model for Screening Antiatherosclerosis Drugs

Huoshanmycins A‒C, New Polyketide Dimers Produced by Endophytic Streptomyces sp. HS-3-L-1 From Dendrobium huoshanense

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