Lower methionine/cystine ratio in low‐protein diet improves animal reproductive performance by modulating methionine cycle

Chen, Xingping; Chen, Ting; Sun, Jiajie; Luo, Junyi; Liu, Jie; Zeng, Bin; Zhang, Yongliang; Xi, Qianyun

doi:10.1002/fsn3.1128

Cited by 13 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Besides, the antioxidant capacity has been indicated to be associated with meat quality traits ( 20 ). A previous study showed that a dietary lower methionine and cysteine ratio in an LP diet improved antioxidant capacity in boar ( 38 ), while the extreme LP diet might not support well the antioxidant defense and ability to combat the stress in Chrysolophus amherstiae ( 39 ). Here, we also found that the LP diets reduced plasma MDA contents and increased the GSH contents in the plasma of finishing pigs.…”

Section: Discussionmentioning

confidence: 99%

Low Protein Diet Improves Meat Quality and Modulates the Composition of Gut Microbiota in Finishing Pigs

Zhu

Yang

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

This study investigated the effect of a low protein (LP) diet on growth performance, nitrogen emission, carcass traits, meat quality, and gut microbiota in finishing pigs. Fifty-four barrows (Duroc × Landrace × Yorkshire) were randomly assigned to three treatments with six replicates (pens) of three pigs each. The pigs were fed with either high protein (HP, 16% CP), medium protein (MP, 12% CP), and LP diets (10% CP), respectively. The LP diets did not influence the growth performance, but significantly decreased the plasma urea nitrogen contents and fecal nitrogen emission (P < 0.05). The LP diet significantly decreased the plasma contents of malondialdehyde (MDA) and increased the plasma glutathione (GSH) contents (P < 0.05). The LP diets significantly increased the backfat thickness at the first and last ribs, L* (lightness) value of meat color, and muscle fiber density in the longissimus dorsi (P < 0.05). The messenger RNA (mRNA) expression of fatty acid synthetase (FAS), peroxisome proliferator-activated receptor-gamma (PPARγ), leptin, and acetyl-CoA carboxylase (ACC) was significantly downregulated, while that of carnitine palmitoyltransferase 1 (CPT1) and myosin heavy chain (MYHC) IIx in the longissimus Dorsi muscle was significantly upregulated by LP diets (P < 0.05). The 16S sequencing analysis showed that the abundance of unidentified Bacteria at the phylum level, and Halanaerobium and Butyricicoccusat at the genus level in the colonic digesta were significantly decreased by LP diet (P < 0.05). The LP diet significantly decreased the observed species of α-diversity in both ileal and colonic microbiota (P < 0.05). Spearman correlation analysis identified a significant positive correlation between the abundance of the ileal genera Streptococcus and L* value at 24 and 48 h, and a significant negative correlation between unidentified_Ruminococcasceae in both ileum and colon with L* value at 24 h (P < 0.05). Collectively, the LP diet supplemented with lysine, methionine, threonine, and tryptophan could reduce the fecal nitrogen emission without affecting growth performance and improve meat quality by regulating the antioxidant capacity and gene expression involved in fat metabolism as well as modulating the gut microbiota composition in finishing pigs.

show abstract

Section: Discussionmentioning

confidence: 99%

Low Protein Diet Improves Meat Quality and Modulates the Composition of Gut Microbiota in Finishing Pigs

Zhu

Yang

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Means and standard errors of means were calculated for all parameters. Statistical differences among bulls were obtained using one-way analysis of variance with Duncan's multiple range test (SPSS 21.0) and p<0.05 was considered statistically significant [29].…”

Section: Discussionmentioning

confidence: 99%

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

et al. 2020

View full text Add to dashboard Cite

Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls. Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen. Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.

show abstract

“…The sperm suspension was collected and filtered through a nylon mesh with a 38-μm pore diameter to remove tissue fragments and then used to record and calculate sperm count and abnormality according to the methods in a previous study ( Guo et al, 2019 ). The types of abnormal sperm morphology observed in this study mainly included the folded-tail, hookless, amorphous, double-head and double-tail phenotypes according to a previous study ( Chen et al, 2019 ). In addition, a FITC annexin V apoptosis detection kit Ι (BD Pharmingen, CA, United States) was applied to quantify the survival rate and apoptosis rate of sperm.…”

Section: Methodsmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

View full text Add to dashboard Cite

To investigate whether the abscopal effects of cranial irradiation (C-irradiation) cause testicular damage in mice, male C57BL/6 mice (9weeks of age) were randomly divided into a sham irradiation group, a shielded group and a C-irradiation group and administered sham/shielded irradiation or C-irradiation at a dose rate of 2.33Gy/min (5Gy/d for 4 d consecutively). All mice were sacrificed at 4weeks after C-irradiation. We calculated the testis index, observed testicular histology by haematoxylin-eosin (HE) staining and observed testicular ultrastructure by transmission electron microscopy. Western blotting was used to determine the protein levels of Bax, Bcl-2, Cleaved caspase 3, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in the testes of mice. Immunofluorescence staining was performed to detect the expression of Cleaved caspase 3 and 3β hydroxysteroid dehydrogenase (3βHSD), and a TUNEL assay was used to confirm the location of apoptotic cells. The levels of testosterone (T), GDNF and SCF were measured by ELISA. We also evaluated the sperm quality in the cauda epididymides by measuring the sperm count, abnormality, survival rate and apoptosis rate. The results showed that there was no significant difference in testicular histology, ultrastructure or sperm quality between the shielded group and sham group. Compared with the sham/shielded group, the C-irradiation group exhibited a lower testis index and severely damaged testicular histology and ultrastructure at 4weeks after C-irradiation. The levels of apoptosis in the testes increased markedly in the C-irradiation group, especially in spermatogonial stem cells. The levels of serum T and testicular 3βHSD did not obviously differ between the sham group and the C-irradiation group, but the levels of GDNF and SCF in the testes increased in the C-irradiation group, compared with the sham group. In addition, the sperm count and survival rate decreased in the C-irradiation group, while the abnormality and apoptosis rate increased. Under these experimental conditions, the abscopal effects of C-irradiation induced testicular damage with regard to both structure and function and ultimately decreased sperm quality in mice. These findings provide novel insights into prevention and treatment targets for male reproductive damage induced by C-irradiation.

show abstract

Lower methionine/cystine ratio in low‐protein diet improves animal reproductive performance by modulating methionine cycle

Cited by 13 publications

References 31 publications

Low Protein Diet Improves Meat Quality and Modulates the Composition of Gut Microbiota in Finishing Pigs

Low Protein Diet Improves Meat Quality and Modulates the Composition of Gut Microbiota in Finishing Pigs

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

Contact Info

Product

Resources

About