&lt;b&gt;Characterization of race 65 of &lt;i&gt;Colletotrichum lindemuthianum&lt;/i&gt; by sequencing ITS regions

Coêlho, Marcela; Gonçalves‐Vidigal, Maria Celeste; Sousa, Lorenna Lopes de; Nunes, Maria Paula; Azevedo, Rafhael Felipin; Galván, Marta Zulema

doi:10.4025/actasciagron.v38i4.30586

Cited by 10 publications

(10 citation statements)

References 24 publications

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“…On the other hand, races 65 and 73 are the most frequent and widespread races in Brazil (Thomazella, Gonçalves‐Vidigal, Vidigal Filho, Nunes, & Vida, 2002a; Gonçalves‐Vidigal, Thomazella, Vidigal Filho, Kvitschal, & Elias, 2008; Pereira, Ishikawa, Pinto, & Souza, 2010; Damasceno e Silva, Souza, & Ishikawa, 2007; Xavier et al., 2018). Previous studies revealed that race 65 presented high variability in several states of Brazil (Coêlho et al., 2016; Ishikawa, Souza, & Davide, 2008; Rosada et al., 2010; Santos, Antunes, Rey, & Rossetto, 2008; Thomazella et al., 2002b). Race 9 has been reported on the African and American continents, especially in countries such as Brazil, Colombia, Peru, Mexico, Guatemala, Honduras, Costa Rica, the Dominican Republic, and Argentina (Balardin et al., 1997; Mahuku & Riascos, 2004; Nunes et al., 2013; Pastor‐Corrales et al., 1995; Souza et al., 2016).…”

Section: Discussionmentioning

confidence: 96%

Genome‐wide association study of resistance to anthracnose and angular leaf spot in Brazilian Mesoamerican and Andean common bean cultivars

et al. 2020

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Anthracnose (ANT) and angular leaf spot (ALS) are devastating diseases of common bean (Phaseolus vulgaris L.) worldwide, and identification of new sources of ANT and ALS resistance is a major priority in breeding programs. The ANT and ALS reaction of 57 Andean and 58 Mesoamerican accessions from five Brazilian states were evaluated. Three Mesoamerican and eight Andean accessions were resistant to races 9, 65, 73, 2047, and 3481 of the ANT pathogen, while four Mesoamerican and 14 Andean accessions were resistant to four of the ANT pathogen races. Furthermore, 13 Mesoamerican and 30 Andean accessions were resistant to races 31‐23 and 63‐39 of the ALS pathogen. To understand the genetic basis of ANT and ALS resistance, a genome‐wide association study (GWAS) was conducted using the 115 accessions, revealing new sources of resistance in Andean and Mesoamerican accessions. The single nucleotide polymorphism (SNP) markers associated with races 9 and 73 of ANT was positioned on chromosome Pv04; resistance to race 65 on chromosomes Pv01, Pv04, and Pv08; and resistance to races 2047 and 3481 on chromosomes Pv10 and Pv05, respectively. Furthermore, SNPs associated with race 31‐23 of Pseudocercospora griseola (Sacc.) Crous & U. Braun were mapped on chromosomes Pv02 and Pv04, whereas for race 63‐39, SNPs were mapped on chromosomes Pv03, Pv06, and Pv08. The SNP markers and candidate genes found associated with the resistance should be validated in segregating populations, which could further be used for marker‐assisted selection. As a result, breeding programs might be able to develop resistant bean cultivars to ANT and ALS.

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Section: Discussionmentioning

confidence: 96%

Genome‐wide association study of resistance to anthracnose and angular leaf spot in Brazilian Mesoamerican and Andean common bean cultivars

et al. 2020

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“…Currently, data regarding molecular variability of C. lindemuthianum patotypes is scarce, except for some races. For example, Talamini et al (2006), Davide and Souza (2009) and Coêlho et al (2016) reported the presence of molecular variability between and within isolates belonging to race 65. Another investigation also revealed pathogenic variability of races 65, 73 and 81 (Santos, Antunes, Rey & Rossetto, 2008).…”

Section: Intra-race Variability Based On Sequencing Of the Its1 58s And Its2 Regionsmentioning

confidence: 99%

“…These regions are transcribed and processed to originate the ribosomal RNA (Fungaro, 2000). Several genetic diversity studies have shown the efficiency of ITS markers to carry out homology sequence comparison in different phytopathogens (Torres, Ganal & Hemleben, 1990;Balardin, Smith & Kelly, 1999;Navajas, Lagnel, Fauvel & Moraes, 1999;Schoch et al, 2012;Coêlho et al, 2016;Martiniano-Souza et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Colletotrichum lindemuthianum races based on ITS-rDNA regions

Coêlho

Vidigal

Filho

et al. 2020

ASB J.

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Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean. Favorable conditions for this disease might result in up to 100% yield losses. One of the main challenges for common bean producers and breeders still remains the management disease, since this pathogen exhibits a wide genetic variability probably due to its recombination sexual reproduction. The 5·8S gene and the flanking internal transcribed spacer regions (ITS1 and ITS2) of 40 different isolates of C. lindemuthianum collected in Brazil were amplified by PCR, and sequenced in order to determine genetic variability. The results revealed that 46.88% of SNPs were detected in the ITS1 region, while 53.12% of them were located in the ITS2 region. The genetic distance ranged from 0.000 to 0.169 between races. The greatest distance was observed between the races 10 and 73 with a value of 0.169, indicating a wide genetic variability between them. The phylogenetic tree was composed of three groups. Group I had five subgroups. Similar results were also observed through population structure analysis, which revealed the presence of three clusters. These results suggest that sequence analysis of ITS rDNA regions of C. lindemuthianum may be a valuable tool to identify this pathogen through design of specific primers.

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“…In addition to the characterization of C. lindemuthianum races, studies at the molecular level have contributed to a better understanding of the variability of this fungus (Balardin et al., 1997; Mesquita et al., 1998). For instance, the genetic variability between and within races or isolates of C. lindemuthianum has been studying through random amplified polymorphic DNA (RAPD), sequencing of internal transcribed spacer, amplified fragment length polymorphism, and restriction fragment length polymorphism markers(Ansari et al., 2004; Balardin et al., 1997; Balardin et al., 1999; Coêlho et al., 2016; Damasceno Silva et al., 2007; Fabre et al., 1995; González et al., 1998; Mesquita et al., 1998; Sicard et al., 1997). The accessibility of genome sequences and the development of molecular technologies allowed the use of markers for marker‐assisted selection applied to ANT‐resistant cultivars (Schmutz et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

Relationship of Colletotrichum lindemuthianum races and resistance loci in the Phaseolus vulgaris L. genome

et al. 2021

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Anthracnose caused by Colletotrichum lindemuthianum is one of the most critical diseases in the common bean (Phaseolus vulgaris L.). The characterization and localization of pathogenic fungal races are essential for understanding pathogen population dynamics and developing resistant cultivars. Here, we discussed the relationship between the diversity of C. lindemuthianum and common bean resistance genes against anthracnose disease. Indeed, several studies using a system of 12 differential bean cultivars have been carried out since This article is protected by copyright. All rights reserved.1991 to monitor anthracnose, reporting the constant appearance of new races. C. lindemuthianum shows high virulence diversity, with 298 races distributed across 29 countries. In Brazil, were identified 89 races, being races 73, 65, and 81 the most frequent. Moreover, we highlighted the common bean gene clusters, their unique location on chromosomes, and their relationship with races and resistance genes. The genetic mapping studies, molecular markers linked to ANT resistance alleles and the specific races were integrated on the seven-chromosome map using the reference genome of common bean.Recently, the ease of use of genome sequences and the development of molecular technologies have allowed molecular markers for marker-assisted selection applied to anthracnose-resistant cultivars. As a result, it is feasible that Mesoamerican genes , and the Andean genes Co-1 2 , Co-1 4 , Co-Bf, Co-15, Co-AC, and CoPv01 CDRK are well-known to confer resistance to most races reported in Brazil and around the world. Thus, pyramiding these genes through molecular markers can help reduce the time and cost of introducing resistance genes in commercial common bean cultivars. | INTRODUCTIONCommon bean (Phaseolus vulgaris L.) is a crucial staple food. It is one of the primary sources of carbohydrates, proteins, vitamins, and minerals worldwide and is cultivated in approximately 100 countries (Broughton et al., 2003;Vaz Patto et al., 2015). Among the factors that affect bean productivity, anthracnose (ANT) caused by the fungus Colletotrichum lindemuthianum (Sacc. & Magn.) Bri. & Cav. is considered one of the most serious ones. In fact, under C. lindemuthianum favorable conditions, anthracnose can reduce bean production

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<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

Cited by 10 publications

References 24 publications

Genome‐wide association study of resistance to anthracnose and angular leaf spot in Brazilian Mesoamerican and Andean common bean cultivars

Genome‐wide association study of resistance to anthracnose and angular leaf spot in Brazilian Mesoamerican and Andean common bean cultivars

Genetic diversity of Colletotrichum lindemuthianum races based on ITS-rDNA regions

Relationship of Colletotrichum lindemuthianum races and resistance loci in the Phaseolus vulgaris L. genome

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