<i>In vitro</i> antibacterial activity of diterpene and benzoxazole derivatives from <i>Excoecaria agallocha</i> L.

Abstract

The in vitro antibacterial activity of column chromatographic fractions of n-hexane, benzene, chloroform, acetone, ethanol and water extracts of Excoecaria agallocha L. were determined against 24 localized and common bacterial pathogens. Antibacterial assay was performed by agar diffusion method against 4 specialized urinary tract pathogens, 10 antibiotic sensitive ophthalmic bacterial pathogens, 5 antibiotic resistant bacterial pathogens and 5 fish pathogens. It was found that the 11 th fraction of chloroform extract and the 30 th fraction of water extract exhibited broad spectrum of antibacterial activity. These 2 active fractions were further investigated through UV-Visible, NMR and FT-IR and were characterized as benzoate and diterpenes and their derivatives viz., 2,3-secoatisane type diterpene and 3,4,5-trihydroxy methyl benzoate.

Mangrove plants as a source of lead compounds for the development of new antiplasmodial drugs from South East coast of India

“…The in vitro antiplasmodial activity of the A. marina plant extract may due to the presence of major chemical classes, such as phenols and coumarins, Hence, coumarins are strong antiplasmodial compounds (Murakami et al 1994;Sherman and Billing 1999). Previous studies on mangrove plant parts and its major chemical classes displayed various level of biological activities, such as antibacterial, antifungal, antiplasmodial, cytotoxic, antifouling, hepatoprotective, ichtyotoxic, cytotoxic, and free radical scavenging activities (Ravikumar et al 2009(Ravikumar et al , 2010aRaja et al 2010;Manilal et al 2009;Agoramoorthy et al 2008). Except the coumarins, the major chemical classes, such as alkaloids, phenols, polysaccharides, and flavanoids also exerted strong antiplasmodial activities (Basak et al 1996;Scalbert 1991;Cowan 1999).…”

Anticancer property of sediment actinomycetes against MCF–7 and MDA–MB–231 cell lines

“…Actinomycetes are one of the most important sources for new bioactive compounds such as antibiotics and enzymes [6][7][8] which have diverse clinical effects against many pathogenic organisms viz., bacteria, fungi and parasite, etc. In fact, more than 60% of approved drugs are derived from natural compounds [9] in that, 50% of the natural antibiotics are produced from actinomycetes, among them marine actinomycetes have been reported to have antifungal, antibacterial and antiinflammatory activities [10] . But, studies related with the biopotential activities of anticancer metabolites from marine actinomycetes based drug discovery are too limited.…”

Insight on Excoecaria agallocha: An Overview

“…Among those strains, the ethanolic extract of E. agallocha exhibited potential antibacterial activity against Acremonium strictum, and Pnicellium expansum then compared to others [25]. The chloroform and water extracts from leaves of E. agallocha showed potential activity against urinary tract pathogens, antibiotic sensitive ophthalmic bacterial pathogens, antibiotic resistant bacterial strains and fish pathogen [26]. Staphylococcus aureus is a multidrug resistant pathogenic bacterial strain.…”