<i>In vitro</i> tuberization of potato influenced by benzyl adenine and chloro choline chloride

Abstract

In an experiment, six levels of benzyl adenine (0.2. 5,5.0,7.5,lO.0,12.5 and 15.0 mg/I) in combination with three levels of (CCC) chloro choline chloride (0,125,250 and 500 mg/I) were evaluated against control treatment (0) in each case to find out their optimum levels for microtuberization in potato variety Diarnant. Microtuberization was the earliest by I 3 days at 10 mg/I BA. The number and average weight of microtuber per flask vas increased with increasing rate of BA and reached maximum of 12.9 and 252.1 mg respectively at 10 mg/I BA and decreased onwards with further increase of BA concentration. The increase rate of CCC increased the number of microtuber but decreased average weight. The maximum number and average weight of microtuber were recorded at 500 mg/I CCC and in absence of CCC respectively.

Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

“…These different works show that, the percentage of plantlets producing microtubers, the number of microtubers per plantlet and their size varies not only according to species but also according to the culture conditions and this is confirmed in this study. In fact, jasmonic acid has been shown to be effective on microtuberization (Santos and Salema, 2000), but this effectiveness also depend on the other culture conditions mainly the type and the concentration of phytohormones used and the concentration of sucrose in the culture media (Hoque, 2010;Yu et al, 2000;Zakaria et al, 2008;Bazabakana et al, 2003). The results of this study shows that, microtubers in Manihot esculenta can be produced in the presence of 2 µM jasmonic acid and 0.1 to 0.6 mg.L -1 NAA or Kin used separately or in ratio (NAA/Kin).…”

Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum)

“…Rapid tuberization with P 2 protocol might be due to the presence of optimum concentrations of BA and CCC in the medium. Microtuberization promoted in presence of CCC (Hossain and Sultana, 1998;Zakaria et al, 2008b) and BA (Islam, 1995 andZakaria et al, 2008b). Protocol P 5 showed earlier tuberization than P 3 which may be due to the presence of high concentration of BA and sucrose.…”

“…It is clear that microtuber number and average weight varied among protocols. The discrepancies between these findings are probably due to the use of different genotypes (Coleman and Coleman, 2000;Zakaria et al, 2008a) different mixtures of nutrient salts, and/or different concentrations of the growth regulators added (Zakaria et al, 2008b), together with possible variation in the size of vessel, light intensity and temperature. Therefore, the present study was undertaken to evaluate the performance of different protocols on in vitro tuberization in different potato varieties and to find out variety specific protocols.…”

Pengaruh Naungan dan Zat Pengatur Tumbuh terhadap Pertumbuhan dan Hasil Tanaman Kentang Kultivar Atlantik di Dataran Medium

“…Aplikasi zat pengatur tumbuh seperti Benzylaminopurine yang dikombinasikan dengan paclobutrazol dan chloro choline chloride (CCC) diharapkan dapat mengatasi permasalahan tersebut. Benzylaminopurine berpengaruh dalam merangsang percabangan dan memacu pertumbuhan daun (Jafari et al, 2011), serta meningkatkan bobot umbi (Zakaria et al, 2008). Hormon pemacu tumbuh benzylaminopurine perlu diberikan pada tanaman kentang pada fase awal pertumbuhan.…”

“…Hasil penelitian Tekalign and Hammes (2005); Hamdani et al (2009) menunjukkan bahwa aplikasi paclobutrazol dapat meningkatkan hasil dan kualitas hasil kentang seperti berat jenis dan bahan kering pada umbi kentang. Menurut Zakaria et al (2008), CCC secara in vitro telah terbukti berpengaruh dalam pembentukan umbi kentang.…”