<p>Antidiabetic Effect of Germinated <em>Lens culinaris Medik</em> Seed Extract in Streptozotocin-Induced Diabetic Mice</p>

Abstract

Background: Lens culinaris Medik seed has been used in traditional practices to treat various ailments, including diabetes mellitus, in Ethiopia. Previous phytochemical screening studies indicated that germination of the seed of L. culinaris contains more bioactive constituents compared to raw seeds. The aim of this study was to investigate the antidiabetic activity of an aqueous methanol extract of germinated L. culinaris seed extract in streptozotocin (Stz)-induced diabetic mice. Methods: The antidiabetic effect of germinated L. culinaris seed extract was determined using Stz-induced diabetic mice. An 80% aqueous methanol extract of germinated L. culinaris seed at doses of 100, 200, and 400 mg/kg was used in the treatment group. Glibenclamid (5 mg/ kg) and dimethyl sulfoxide 2% were used as positive and negative controls, respectively. The test extract and controls were given daily for 3 weeks. Blood-glucose levels and bodyweight changes were measured weekly. Lipid-profile levels were measured at the end of each experiment. Oral glucose-tolerance tests were performed to evaluate the postprandial effect of the extract. Results: The aqueous methanol extract of germinated L. culinaris significantly reduced blood-glucose levels and increased body weight (p<0.05). The extract also improved serumlipid profiles in diabetic mice after 21 days (p<0.05). The seed extract also resulted in significant reductions in blood-glucose levels after an oral glucose load in normal mice (p<0.05). Conclusion: An aqueous methanol extract of germinated L. culinaris seed has both antidiabetic and antihyperlipidemic effects.

Comparative Insights into Four Major Legume Sprouts Efficacies for Diabetes Management and Its Complications: Untargeted versus Targeted NMR Biochemometrics Approach

“…Additionally, the bar loading plot ( Figure 4 C) confirmed the prominent effect of TFC, followed by L-DOPA and betaine. Flavonoids have been well documented for their antidiabetic effect in foods and, more specifically, in legume sprouts, including extract of Lens culinaris and associated serum-lipid profiles [ 14 ], and Cicer arietinum extracts via their antioxidant and intestinal α -glucosidase inhibitory effects [ 13 ].…”

“…Various studies have reported legumes’ antidiabetic action or their germinated seeds [ 13 , 14 ] and suggested mechanisms underlying the antidiabetic effect are mediated through delaying gastric emptying and inhibition of carbohydrate digestive enzymes. Moreover, seeds rich in trigonelline protect pancreatic β -cells, increase both insulin level and sensitivity [ 15 ], and stimulate an insulin signaling pathway [ 9 ].…”

Protective effects of a standardized extract of Iris germanica on pancreas and liver in streptozotocin-induced diabetic rats

“…Diabetes was induced by a single intraperitoneal injection of STZ (Sigma, St. Louis, USA) at a dose of 65 mg/kg. After one week, the level of FBG was determined using an Accu-check active glucometer (Roche, Mannheim, Germany) and the rats with FBG ≥ 200 mg/dL were enrolled in diabetic groups ( 16 17 ). The extract was administrated by oral gavage once daily for 4 weeks.…”

Protective Effects of Medicinal Plant-Based Foods against Diabetes: A Review on Pharmacology, Phytochemistry, and Molecular Mechanisms

“…Lentils contain polyphenols, phytates, triterpenoids, defensins, phytosterols, flavonoids, saponins, protease inhibitors, dietary fibers, and lectins, among which saponins and polyphenols are potent radical scavengers and HMG-CoA reductase inhibitors [ 114 ].…”