&lt;p&gt;Apoptotic and DNA-damaging effects of yttria-stabilized zirconia nanoparticles on human skin epithelial cells&lt;/p&gt;

Alzahrani, Fatimah Mohammed; Katubi, Khadijah Mohammedsaleh; Ali, Daoud; Alarifi, Saud

doi:10.2147/ijn.s212255

Cited by 23 publications

(17 citation statements)

References 41 publications

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“…Our results showed that ZrO 2 -NPs triggered cytotoxicity in a dose-and time-dependent manner. Our findings were consistent with those of previous studies reported recently [7,8,36] . Figure 8B.…”

Section: In Vitro Studysupporting

confidence: 94%

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Toxicity of Zirconia oxide Nanoparticles: Liver Biodistribution and Liver Damages

Sun

Zhan

et al. 2020

Preprint

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Background: Zirconia nanoparticles (ZrO2-NPs) have been increasingly used in industrial, biomedical and dental materials. However, the scientific basis for the toxicological effects of ZrO2-NPs is poorly elucidated, and the understanding of the underlying mechanism is still limited. Results: The hepatic biodistribution and toxicological effects of ZrO2-NPs after intravenous administration (20mg/kg bw) in vivo and the toxicological mechanism toward hepatocytes in vitro were investigated. The liver showed continuous ZrO2-NP accumulations liver over a 28-d period. Moreover, ZrO2-NPs induced oxidative stress and increased inflammatory responses and functional injury in the liver. Hepasteatosis and cell death were observed in histopathological and immunohistochemical studies. RNA-seq identified the main pathways involved in the metabolism, cellular process, and human diseases. The RT-qPCR analysis results showed that ZrO2-NP exposure caused the upregulation of P53, Foxo1, Gadd45g, P21, Caspase3, and PPARα and the downregulation of Igfbp2 and Akt in the liver in response to the ZrO2-NP treatment. Meanwhile, the results of the in vitro studies demonstrated that ZrO2-NPs exposure resulted in cytotoxicity in Hepg2 cells in a dose- and time-dependent manner. ZrO2-NPs were proven to induce oxidative stress, lipid accumulation, cell cycle arrest and cell apoptosis to Hepg2 cells. Western-blot analysis further proved the depression of Igfbp2, activation of Akt-mediated signaling pathway and P53-mediated signaling pathway for Hepg2 cells exposure to ZrO2-NPs. Conclusions: This study proves that ZrO2-NPs have negative impacts on the liver and exhibit potential risks for non-alcoholic fatty liver disease (NAFLD). There is potential concern over ZrO2-NPs' hepatoxicity in biomedical applications and occupational exposure through large-scale production.

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Section: In Vitro Studysupporting

confidence: 94%

“…Studies have proven the potential toxicity of ZrO 2 -NPs. Alzahrani et al [7] reported the apoptotic and DNA-damaging effects of yttria-stabilized ZrO 2 -NPs on human skin epithelial cells. Ye et al [8] showed that ZrO 2 -NPs could induced ROS productions and apoptosis effects, which may have an impact on osteogenesis in Osteoblast-Like 3T3-E1 Cells.…”

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confidence: 99%

Toxicity of Zirconia oxide Nanoparticles: Liver Biodistribution and Liver Damages

Sun

Zhan

et al. 2020

Preprint

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“…Generation of intracellular ROSin CHANG and HuH-7 cells after exposure to gAgNPs (0, 5, 20, 40 and 100 µg/mL) for 24 hrs was determined according to the methods described Alzahrani et al 24 . Briefly, 1×10 4 cells were cultured in black bottom culture plate (96 well) and left 24 h for attachment in CO 2 incubator at 37°C.…”

Section: Evaluation Of Intracellular Reactive Oxygen Species (Ros)mentioning

confidence: 99%

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

Bin-Jumah¹,

Al-Abdan²,

Albasher³

et al. 2020

IJN

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Introduction: Extensive use of metallic nanomaterials in different areas of agriculture and commercial products induce significant harmful effects on human health and the environment. In the current study, we synthesized an eco-friendly approach silver nanoparticles (AgNPs) using root extracts of Beta vulgaris L. Methods: The synthesized green silver nanoparticles (gAgNPs) were characterized by dynamic light scattering (DLS) and high-resolution transmission electron microscope (HR-TEM). The gAgNPs had a round shape and the mean size was 20−50 nm. The cytotoxic effects of gAgNPs were determined in human hepatic normal (CHANG) and cancer (HUH-7) cells by using tetrazolium salt (MTT) and lactate dehydrogenase (LDH) assays for 24 h. Results and Discussion: It was clear from the observations of this experiment that higher concentrations of gAgNPs reduce cell viability. The production of reactive oxygen species (ROS) was evaluated by using DCFDA. The gAgNPs induced more ROS in the HuH-7 cells than in the CHANG cells. The fragmentation of DNA was evaluated by alkaline single-cell gel electrophoresis and the maximum DNA strand breakage was found at a higher concentration exposure of gAgNPs for 24 h. It is important to notice that the HuH-7 cells showed an increased sensitivity to gAgNPs than the CHANG cells. The apoptotic and necrotic effects of gAgNPs on both the cells were evaluated using annexin-V-FITC and propidium iodide staining. An increased count of apoptotic and necrotic cells was found following a higher concentration exposure of gAgNPs. The apoptotic protein expression in these cells due to gAgNPs exposure was determined using immunoblotting techniques and the level of Bcl2 was decreased. However, the expression of BAX and protein was increased in both cells. Conclusion: Therefore, it can be concluded that higher concentrations of gAgNPs may induce significant cytotoxicity and cause DNA damage and apoptosis.

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“…High concentrations and long incubation periods caused prominent oxidative stress, mitochondrial damage, and an apoptotic cell death mode in addition to DNA strand breakage. [ 41 ]…”

Section: Resultsmentioning

confidence: 99%

Role of nano‐zirconia in the mechanical properties improvement of resin cement used for tooth fragment reattachment

et al. 2021

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Dental fractures of the anterior teeth are a common form of dental trauma. Reattachment of fractured tooth fragment by means of self‐adhesive resin cement is currently compromised by the poor mechanical properties of these ceramics. Herein, we synthesized zirconium dioxide nanoparticles (nano‐ZrO2) using the Co‐precipitation approach and then mixing it in different concentrations (1.0, 2.0, 3.0, and 5.0 wt%) with the commercially available RelyX™ Unicem self‐adhesive universal resin cement (ARC) forming, nano‐ZrO2/ARC. Both the synthesized nano‐ZrO2 and nano‐ZrO2/ARC were characterized using a high‐resolution transmission electron microscope (HR‐TEM), field‐emission scanning electron microscope, X‐ray diffraction, and Fourier transform infrared technique. The HR‐TEM images revealed that the synthesized nano‐ZrO2 was spherical with a size of ~ 12 nm. The results showed that the addition of nano‐ZrO2 significantly enhanced the mechanical properties as fracture resistance, compressive strength, diametral tensile, and micro‐hardness values of the resin cement. The mixing 3.0 wt% of nano‐ZrO2 with ARC revealed the maximum improvement in the mechanical properties and, consequently, the resin's repair strength. Their incorporation with ARC up to 3.0% wt did not cause significant cell death than ARC alone. We believe that the information discussed here might be relevant to the stability of nano‐ZrO2‐modified resin cement and, therefore, to its clinical usage for fragment tooth reattachment, cementation of dental crowns, bridges, and bands.

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<p>Apoptotic and DNA-damaging effects of yttria-stabilized zirconia nanoparticles on human skin epithelial cells</p>

Cited by 23 publications

References 41 publications

Toxicity of Zirconia oxide Nanoparticles: Liver Biodistribution and Liver Damages

Toxicity of Zirconia oxide Nanoparticles: Liver Biodistribution and Liver Damages

<p>Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro</p>

Role of nano‐zirconia in the mechanical properties improvement of resin cement used for tooth fragment reattachment

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