<p>Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N-</em>hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds</p>
Abstract:Purpose: A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration. Materials and methods: We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fiber… Show more
“…Protein spectra are characterized by amide stretching and bending vibrations. As can be seen, there is no noticeable shift in the amide peaks among the spectrum patterns of the amniotic membrane powder and the HAMF film, indicating that there were no major changes in the functional groups and the nativity of the proteins such as collagen molecules before and after dissolution 21 , 23 . Figure 2 FTIR spectra of the amniotic membrane powder and the film resulting from its dissolution in the solvent FA/AA (70/30).…”
Section: Resultsmentioning
confidence: 95%
“…3.2 , which can increase the voltage between the positive and negative poles, which forces the jet to spray lower diameter fibers. Several studies have reported that the combination of PCL and collagen reduces the fiber diameter 23 , 31 . With increasing the HAMP percentage in PCL-20HAMP and PCL-30HAMP, the viscosity and the average diameter of the fibers increased.…”
The common retinal diseases are age-related macular degeneration (AMD) and retinitis pigmentosa (RP). They are usually associated with the dysfunction of retinal pigment epithelial (RPE) cells and degeneration of underlying Bruchâs membrane. The RPE cell transplantation is the most promising therapeutic option to restore lost vision. This study aimed to construct an ultrathin porous fibrous film with properties similar to that of native Bruchâs membrane as carriers for the RPE cells. Human amniotic membrane powder (HAMP)/Polycaprolactone (PCL) scaffolds containing different concentrations of HAMP were fabricated by electrospinning technique. The results showed that with increasing the concentration of HAMP, the diameter of fibers increased. Moreover, hydrophilicity and degradation rate were improved from 119° to 92° and 14 to 56% after 28 days immersion in phosphate-buffered saline (PBS) solution, respectively. All scaffolds had a porosity above 85%. Proper cell adhesion was obtained one day after culture and no toxicity was observed. However, after seven days, the rate of growth and proliferation of ARPE-19 cells, a culture model of RPE, on the PCL-30HAMP scaffold (HAMP concentration in PCL 7.2% by weight) was higher compared to other scaffolds. These results indicated that PCL-30HAMP fibrous scaffold has a great potential to be used in retinal tissue engineering applications.
“…Protein spectra are characterized by amide stretching and bending vibrations. As can be seen, there is no noticeable shift in the amide peaks among the spectrum patterns of the amniotic membrane powder and the HAMF film, indicating that there were no major changes in the functional groups and the nativity of the proteins such as collagen molecules before and after dissolution 21 , 23 . Figure 2 FTIR spectra of the amniotic membrane powder and the film resulting from its dissolution in the solvent FA/AA (70/30).…”
Section: Resultsmentioning
confidence: 95%
“…3.2 , which can increase the voltage between the positive and negative poles, which forces the jet to spray lower diameter fibers. Several studies have reported that the combination of PCL and collagen reduces the fiber diameter 23 , 31 . With increasing the HAMP percentage in PCL-20HAMP and PCL-30HAMP, the viscosity and the average diameter of the fibers increased.…”
The common retinal diseases are age-related macular degeneration (AMD) and retinitis pigmentosa (RP). They are usually associated with the dysfunction of retinal pigment epithelial (RPE) cells and degeneration of underlying Bruchâs membrane. The RPE cell transplantation is the most promising therapeutic option to restore lost vision. This study aimed to construct an ultrathin porous fibrous film with properties similar to that of native Bruchâs membrane as carriers for the RPE cells. Human amniotic membrane powder (HAMP)/Polycaprolactone (PCL) scaffolds containing different concentrations of HAMP were fabricated by electrospinning technique. The results showed that with increasing the concentration of HAMP, the diameter of fibers increased. Moreover, hydrophilicity and degradation rate were improved from 119° to 92° and 14 to 56% after 28 days immersion in phosphate-buffered saline (PBS) solution, respectively. All scaffolds had a porosity above 85%. Proper cell adhesion was obtained one day after culture and no toxicity was observed. However, after seven days, the rate of growth and proliferation of ARPE-19 cells, a culture model of RPE, on the PCL-30HAMP scaffold (HAMP concentration in PCL 7.2% by weight) was higher compared to other scaffolds. These results indicated that PCL-30HAMP fibrous scaffold has a great potential to be used in retinal tissue engineering applications.
“…Differently from PBS, weight loss did not reach a plateau but steadily increased during the whole period, reaching a maximum of 40% after 28 days. Other research groups report faster rates for PCL/Gel electrospun scaffolds degraded in collagenase solutions, with weight losses around 70â80% after just 14 days (Chen et al, 2019 ; Eskandarinia et al, 2020 ). This discrepancy of results can be explained with the crosslinking strategies that have or have not been adopted.…”
Abdominal hernia repair is a frequently performed surgical procedure worldwide. Currently, the use of polypropylene (PP) surgical meshes for the repair of abdominal hernias constitutes the primary surgical approach, being widely accepted as superior to primary suture repair. Surgical meshes act as a reinforcement for the weakened or damaged tissues and support tissue restoration. However, implanted meshes could suffer from poor integration with the surrounding tissues. In this context, the present study describes the preliminary evaluation of a PCL-Gel-based nanofibrous coating as an element to develop a multicomponent hernia mesh device (meshPCL-Gel) that could overcome this limitation thanks to the presence of a nanostructured biomimetic substrate for enhanced cell attachment and new tissue formation. Through the electrospinning technique, a commercial PP hernia mesh was coated with a nanofibrous membrane from a polycaprolactone (PCL) and gelatin (Gel) blend (PCL-Gel). Resulting PCL-Gel nanofibers were homogeneous and defect-free, with an average diameter of 0.15 ± 0.04 ÎŒm. The presence of Gel decreased PCL hydrophobicity, so that membranes average water contact angle dropped from 138.9 ± 1.1° (PCL) to 99.9 ± 21.6°, while it slightly influenced mechanical properties, which remained comparable to those of PCL (E = 15.7 ± 2.7 MPa, ÏR= 7.7 ± 0.6 ΔR = 118.8 ± 13.2%). Hydrolytic and enzymatic degradation was conducted on PCL-Gel up to 28 days, with maximum weight losses around 20 and 40%, respectively. The meshPCL-Gel device was obtained with few simple steps, with no influences on the original mechanical properties of the bare mesh, and good stability under physiological conditions. The biocompatibility of meshPCL-Gel was assessed by culturing BJ human fibroblasts on the device, up to 7 days. After 24 h, cells adhered to the nanofibrous substrate, and after 72 h their metabolic activity was about 70% with respect to control cells. The absence of detectable lactate dehydrogenase in the culture medium indicated that no necrosis induction occurred. Hence, the developed nanostructured coating provided the meshPCL-Gel device with chemical and topographical cues similar to the native extracellular matrix ones, that could be exploited for enhancing the biological response and, consequently, mesh integration, in abdominal wall hernia repair.
“…Polycaprolactone is well known as a strong, non-toxic, low degradable, and semi-crystalline polymer. 9,10 Despite the advantages of PCL, but due to the lack of elasticity, it has limitations in STE applications in the form of a neat structure. Therefore, hybrid PCL-based structures of a blend of two or more polymers are suggested to overcome the limitations of PCL for successful clinical applications.…”
The main objective of soft tissue engineering (STE) is to improve the quality of life by replacing damaged tissues with synthetic scaffolds. Collagen is the key structural protein of the native extracellular matrix with nano-scale diameter. Accordingly, this study focused on the fabrication of the blended and co-electrospinning hybrid synthetic polycaprolactone/polyurethane (PCL/PU) nanofibers scaffolds and investigates their in-vivo biocompatibility for the development of their clinical applications in STE. The body reactions were evaluated by examination of the edema, foreign body granulomatous reaction, granulation tissue formation, inflammation, calcification, fibroblastic change, and necrosis as the most important healing effects in pathology examination. The blended and co-electrospinning PCL/PU structures were successfully carried out in the nano-scale range without any beads. According to the histopathological examination of PCL, the scant, mild and mild to moderate, and acute and chronic have been reported for edema, foreign body granulomatous reaction, and inflammation, respectively. Also, severe (for edema), moderate (for foreign body granulomatous reaction), and mild to moderate chronic (for inflammation) were observed for PU. In blended PCL/PU nanofibers, decreasing of the edema and foreign body granulomatous reaction and increasing of the granulation tissue formation and inflammation were observed with the increasing composite ratio of PCL from 25 to 75%. Also in the co-electrospinning hybrid PCL/PU nanofibers, increasing edema, granulation tissue formation, and inflammation were observed by increasing the composite ratio of PU from 25 to 75%. Present study suggested that blended hybrid PCL75/PU25 nanofibers are a promising candidate as synthetic nanofibers scaffolds for STE applications.
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