Abstract:The main objective of soft tissue engineering (STE) is to improve the quality of life by replacing damaged tissues with synthetic scaffolds. Collagen is the key structural protein of the native extracellular matrix with nano-scale diameter. Accordingly, this study focused on the fabrication of the blended and co-electrospinning hybrid synthetic polycaprolactone/polyurethane (PCL/PU) nanofibers scaffolds and investigates their in-vivo biocompatibility for the development of their clinical applications in STE. T… Show more
“…Single-fluid electrospinning, also known as hybrid electrospinning, prepares nanofibers by mixing drugs and polymers directly from a single spinneret. The traditional single-fluid electrospinning technique has attracted tremendous interest from researchers over the years owing to its simple experimental device, lower cost, higher throughput, and ease of manipulation [ 20 , 86 ]. However, hybrid electrospinning has major limitations on the materials prepared—the polymers must be spinnable, which largely limits the development of the process.…”
Chronic diabetic wounds are one of the main complications of diabetes, manifested by persistent inflammation, decreased epithelialization motility, and impaired wound healing. This will not only lead to the repeated hospitalization of patients, but also bear expensive hospitalization costs. In severe cases, it can lead to amputation, sepsis or death. Electrospun nanofibers membranes have the characteristics of high porosity, high specific surface area, and easy functionalization of structure, so they can be used as a safe and effective platform in the treatment of diabetic wounds and have great application potential. This article briefly reviewed the pathogenesis of chronic diabetic wounds and the types of dressings commonly used, and then reviewed the development of electrospinning technology in recent years and the advantages of electrospun nanofibers in the treatment of diabetic wounds. Finally, the reports of different types of nanofiber dressings on diabetic wounds are summarized, and the method of using multi-drug combination therapy in diabetic wounds is emphasized, which provides new ideas for the effective treatment of diabetic wounds.
“…Single-fluid electrospinning, also known as hybrid electrospinning, prepares nanofibers by mixing drugs and polymers directly from a single spinneret. The traditional single-fluid electrospinning technique has attracted tremendous interest from researchers over the years owing to its simple experimental device, lower cost, higher throughput, and ease of manipulation [ 20 , 86 ]. However, hybrid electrospinning has major limitations on the materials prepared—the polymers must be spinnable, which largely limits the development of the process.…”
Chronic diabetic wounds are one of the main complications of diabetes, manifested by persistent inflammation, decreased epithelialization motility, and impaired wound healing. This will not only lead to the repeated hospitalization of patients, but also bear expensive hospitalization costs. In severe cases, it can lead to amputation, sepsis or death. Electrospun nanofibers membranes have the characteristics of high porosity, high specific surface area, and easy functionalization of structure, so they can be used as a safe and effective platform in the treatment of diabetic wounds and have great application potential. This article briefly reviewed the pathogenesis of chronic diabetic wounds and the types of dressings commonly used, and then reviewed the development of electrospinning technology in recent years and the advantages of electrospun nanofibers in the treatment of diabetic wounds. Finally, the reports of different types of nanofiber dressings on diabetic wounds are summarized, and the method of using multi-drug combination therapy in diabetic wounds is emphasized, which provides new ideas for the effective treatment of diabetic wounds.
“…When the electrostatic field achieves the critical voltage, a charged jet ejects from the tip needle and a fine fiber with micro to nano-scale produce on the surface of collector. 76,80 Figure 3.A schematic setup of a standard electrospinning setup.…”
Section: Vascular Tissue Engineering: Historical Perspective Current ...mentioning
confidence: 99%
“…75 Also, their biological properties are analyzed by in-vitro and in-vivo examination by evaluated their toxicity, cell culture and implanted in animal model. 76,77 An electrospinning setup consists of three major components, as shown in Figure 3: a high voltage power supply, a syringe with a metallic needle, and a collector. 78,79 During the electrospinning process, the polymer dissolved into special solvent.…”
Section: Vascular Tissue Engineering: Historical Perspective Current ...mentioning
confidence: 99%
“…Also, their biological properties are analyzed by in-vitro and in-vivo examination by evaluated their toxicity, cell culture and implanted in animal model. 76,77…”
Section: Vascular Tissue Engineering: Historical Perspective Current ...mentioning
The main objective of the present review article is to investigate the in-vitro and in-vivo biocompatibility behavior of hybrid PCL-based scaffolds with collagen, gelatin, and chitosan to improve endothelialization for VTE applications. It is reported that the high-rate failure of small diameters vascular grafts (SDVGs, <6 mm) due to adhesion of platelets and plasma protein and aggregation, over-proliferation of smooth muscle cells (SMCs), and neointimal hyperplasia at the implantation site is main challenge in vascular tissue engineering (VTE). The fast re-establishment of a functional endothelial cell (EC) layer representing a crucial importance strategy has been proposed to reduce these adverse outcomes. Polycaprolactone (PCL), with optimum mechanical properties, it showed great potential in biomedical applications, but its biocompatibility is still highly concerned in VTE. Modifications of the PCL vascular grafts by developing the hybrid structures using natural polymers with optimum hydrophilicity and biocompatibility properties in order to speed up the re-endothelialization process have been proposed over the last years. Analyzing the mentioned results in the present study can offer a better understanding of the benefits and challenges of applying the natural polymer as a thrombotic response upon implantation in clinical trials. Also, it can suggest the PCL-Collagen as the best framework for the fabrication of rationally designed SDVGs for VTE.
“…17 Electrospinning is one of the most widely used methods in the production of pharmaceutical systems. 18,19 The advantage of the electrospinning method compared to other methods is ease and speed of work, high encapsulation efficiency, simultaneous transfer of various therapeutic compounds, and cost-effectiveness. 20 The better features of this technique make it attractive for use in medications for wound healing.…”
Acne is an inflammatory disease of the sebaceous glands. Melittin (Mel) is one of the principal toxic components of bee venom that can cause antibacterial and anti-inflammatory effects. Chitosan is a biodegradable polysaccharide has anti-inflammatory, antimicrobial, and regenerative properties. In this study, chitosan (Ch)/Mel 0.001 and 0.003% nanofibers were fabricated for topical treatment of acne vulgaris. Physicochemical properties of Ch/Mel were evaluated using FTIR and FESEM. Furthermore, encapsulation efficiency, tensile strength, water absorption capacity, enzymatic activity, drug release, antimicrobial, and cell cytotoxicity were assayed. Animal models were exerted to study the impact of Ch/Mel nanofibers on the treatment of Propionibacterium acnes. The FESEM results showed that nanofibers were successfully prepared in nano-scale fiber diameter. The FTIR confirmed the presence of Mel in structures. The addition of melittin to the polymer solution reduced electrical conductivity, induced viscosity and tensile strength of the fibers, and decreased the percentage of the swelling. The Mel loading into Ch/Mel 0.003% was reported at 86.74±1%. This Mel releasing process (89.65%) of Ch/Mel 0.003% was slowly completed during 72 h. The Mel retained its hemolytic activity after being loaded into the polymer structure. The drug toxicity study displayed the lack of any significant toxicity from Ch/Mel nanofibers on normal Human Dermal Fibroblasts (HDF). The Ch/Mel 0.003% achieved the highest growth inhibition in P. acnes in vitro and animal studies, this group showed the greatest reduction in inflammation and redness. The Ch/Mel 0.003% structure is proposed as a suitable topical drug delivery system for treating acne vulgaris.
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