&lt;p&gt;Genetic basis for metronidazole and clarithromycin resistance in&amp;nbsp;&lt;em&gt;Helicobacter pylori&lt;/em&gt;&amp;nbsp;strains isolated from patients with gastroduodenal disorders&lt;/p&gt;

Hashemi, Seyed Jalal; Sheikh, Ahmad Farajzadeh; Goodarzi, Hamed; Yadyad, Mohammad Jaafar; Seyedian, Seyed Saeed; Aslani, Sajad; Assarzadegan, Mohammad-Ali

doi:10.2147/idr.s192942

Cited by 36 publications

(21 citation statements)

References 36 publications

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“…These isolates were considered undetermined clarithromycin-resistant strains. The resistance of these isolates may be related to the existence of mechanisms unrelated to the 23S rRNA gene sequence, such as expression of the RND efflux pumps (resistance nodulation-cell division) and other mutations (e.g., A2142G and T2182C) which were not investigated in our study [27].…”

Section: Discussionmentioning

confidence: 99%

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Vazirzadeh

Falahi

Moghim

et al. 2020

BioMed Research International

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Background and Aims. Helicobacter pylori is a common infectious bacterium mostly found in gastroduodenal diseases. The increased prevalence of clarithromycin-resistant H. pylori strains is a major challenge in the successful treatment of infections caused by this organism. The present study is aimed at detecting the clarithromycin resistance pattern of H. pylori strains isolated from gastric biopsies and evaluating point mutations of the 23S rRNA gene. Patients and methods. In the present descriptive cross-sectional study, 165 patients with gastrointestinal disorders, who were referred to the Endoscopy Center of Dr. Shariati Hospital of Isfahan, Iran, were enrolled from April to July 2018. H. pylori infection was diagnosed by culture, and susceptibility of the isolates to clarithromycin was assessed by the E-test. Minimum inhibitory concentration (MIC) values were obtained based on EUCAST recommendations. Also, fluorescence in situ hybridization (FISH) was used to determine point mutations associated with clarithromycin resistance. Results. By using culturing, H. pylori was isolated from 50.3% (83/165) gastric biopsy specimens. The overall frequency of resistance to clarithromycin was 25.3% (21/83) by the E-test. In the resistance genotypic analysis, 19 isolates had mutations. The prevalence of A2143G and A2144G mutations was 68.4% (13/19) and 31.5% (6/19), respectively. A2143C mutation was not tracked in any isolate. Two isolates with MIC > 0:5 μg/mL had no mutations that could be related to other mechanisms of resistance. Conclusion. As presented in the study, the high prevalence of clarithromycin-resistant H. pylori due to point mutations of the 23S rRNA gene indicates the necessity of revising the standard treatment regimen based on antibiotic susceptibility pattern of each region.

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Section: Discussionmentioning

confidence: 99%

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Vazirzadeh

Falahi

Moghim

et al. 2020

BioMed Research International

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“…11,13 The presence of certain antibiotic resistance genes, particularly rdxA, pbp1A, gyrA, and cla which encode resistance toward metronidazole, amoxicillin, fluoroquinolone, and clarithromycin antibiotic agents, respectively, is one of the most important reasons for occurrence of antibiotic resistance. 14,15 Therefore, it is significant to know the exact phenotypic and genotypic patterns of antibiotic resistance of H. pylori bacteria recovered from foods with animal origins.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat

Mashak¹,

Jafariaskari²,

Alavi³

et al. 2020

IDR

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Background: Foodstuffs with animal origins, particularly meat, are likely reservoirs of Helicobacter pylori. Purpose: An existing survey was accompanied to assess phenotypic and genotypic profiles of antibiotic resistance and genotyping of vacA, cagA, cagE, iceA, oipA, and babA2 alleles amongst the H. pylori bacteria recovered from raw meat. Methods: Six-hundred raw meat samples were collected and cultured. H. pylori isolates were tested using disk diffusion and PCR identification of antibiotic resistance genes and genotyping. Results: Fifty-two out of 600 (8.66%) raw meat samples were contaminated with H. pylori. Raw ovine meat (13.07%) had the uppermost contamination. H. pylori bacteria displayed the uppermost incidence of resistance toward tetracycline (82.69%), erythromycin (80.76%), trimethoprim (65.38%), levofloxacin (63.46%), and amoxicillin (63.46%). All H. pylori bacteria had at least resistance toward one antibiotic, even though incidence of resistance toward more than eight antibiotics was 28.84%. Total distribution of rdxA, pbp1A, gyrA, and cla antibiotic resistance genes were 59.61%, 51.92%, 69.23%, and 65.38%, respectively. VacA s1a (84.61%), s2 (76.92%), m1a (50%), m2 (39.13%), iceA1 (38.46%), and cagA (55.76%) were the most generally perceived alleles. S1am1a (63.46%), s2m1a (53.84%), s1am2 (51.92%), and s2m2 (42.30%) were the most generally perceived genotyping patterns. Frequency of cagA-, oipA-, and babA2-genotypes were 44.23%, 73.07%, and 80.76%, respectively. A total of 196 combined genotyping patterns were also perceived. Conclusion: The role of raw meat, particularly ovine meat, in transmission of virulent and resistant H. pylori bacteria was determined. VacA and cagA genotypes had the higher incidence. CagE-, babA2-, and oipA-H. pylori bacteria had the higher distribution. Supplementary surveys are compulsory to originate momentous relations between distribution of genotypes, antibiotic resistance, and antibiotic resistance genes.

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“…Because of widespread antibiotic use, there has been shown a marked increase of resistance to standard therapy regimen, which currently is a choice of two antibiotics (out of tetracycline, metronidazole, clarithromycin and amoxicillin), proton pump inhibitor and bismuth salt used simultaneously for 10-14 days [6]. There have been reports of multidrugresistant H. pylori strains [14]. Recently, a multitude of possible mechanisms of resistance have been described along genes responsible for the mechanism [15].…”

Section: State Of the Knowledgementioning

confidence: 99%

Expanding the knowledge of Helicobacter pylori – new directions, new challenges

Wronecki¹,

Tywanek²,

Trojanowska³

et al. 2020

J Educ Health Sport

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<p>Genetic basis for metronidazole and clarithromycin resistance in <em>Helicobacter pylori</em> strains isolated from patients with gastroduodenal disorders</p>

Cited by 36 publications

References 36 publications

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

<p>Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of <em>vacA</em>, <em>cagA</em>, <em>iceA</em>, <em>oipA</em>, <em>cagE</em>, and <em>babA2</em> Alleles of <em>Helicobacter pylori</em> Bacteria Isolated from Raw Meat</p>

Expanding the knowledge of Helicobacter pylori – new directions, new challenges

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