&lt;p&gt;Long noncoding RNA THAP9-AS1 is induced by &lt;em&gt;Helicobacter pylori&lt;/em&gt; and promotes cell growth and migration of gastric cancer&lt;/p&gt;

Jia, Wang; Zhang, Jiaqin; Ma, Fengwang; Hao, Shengjie; Li, Xue; Guo, Ruiting; Gao, Qianqian; Sun, Yuan; Jia, Jihui; Li, Wenjuan

doi:10.2147/ott.s201832

Cited by 27 publications

(33 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…THAP9-AS1 is a recently annotated lncRNA and has been shown to be upregulated in pancreatic cancer and neutrophil apoptosis and downregulated in case of septic shock (35)(36)(37) . In this study, we have quantified THAP9 and THAP9-AS1 transcript expression under various stress conditions in the S-phase of cell cycle.…”

Section: Discussionmentioning

confidence: 99%

“…This leads us to speculate that there exists a stress specific regulation of THAP9 and THAP9-AS1 gene-pair that switches to THAP9-AS1 under stress conditions. This also might explain the upregulation of THAP9-AS1 in cancer cells which are naturally stressed (35–37). Moreover, it is possible that both these genes play an unknown role in DNA repair and this might be responsible for their increased expression under certain stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Antisense transcripts and their corresponding sense transcripts often show inverse expression; thus suggesting that one regulates the other (33,34) . There have been recent reports where the THAP9-AS1 lncRNA has been implicated in pancreatic cancer, septic shock and neutrophil apoptosis (35)(36)(37) . This has piqued our interests in studying the relationship between THAP9 and THAP9-AS1.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stress induced Differential Expression of THAP9 & THAP9-AS1 in the S-phase of cell cycle

Sharma

Thakore

et al. 2021

Preprint

View full text Add to dashboard Cite

Transposable elements function as one of the major effectors in response to biological or environmental stress. Under normal conditions, host organisms deploy epigenetic and post-transcriptional machinery (histone modifications, chromatin remodelers, long non-coding RNAs (lncRNAs)) at the TE sites to contain their mobility. But many a times, the chromatin architecture undergoes TE induced changes under the effect of stress that in turn might lead to unprecedented gene expression. LncRNAs are emerging as a crucial tool in the regulation of TEs. TEs possess remarkable abilities to respond in the face of stress, ranging from undetected mutations to changing the regulatory landscape of the host. Although the relationship between stress response and TE activation/deactivation is well acknowledged but our understanding of the mechanism of regulation remains poor. This study focuses on the gene expression of THAP9, a domesticated transposon and lncRNA THAP9-AS1 (THAP9-anti sense1), which form a sense and anti-sense gene pair with a promoter overlap of approximately 350bp. The two genes exhibit different patterns of gene expression under different types of stresses in the S-phase of the cell cycle. THAP9-AS1 is always upregulated under stress whereas THAP9 exhibits both downregulation and upregulation in different stresses. Both THAP9 and THAP9-AS1 exhibit a periodic gene expression throughout the S-phase which is a characteristic of cell cycle regulated genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stress induced Differential Expression of THAP9 & THAP9-AS1 in the S-phase of cell cycle

Sharma

Thakore

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Although the effect of the two lncRNAs in sepsis remains unclear, their roles have been well studied in various human diseases. THAP9-AS1, induced by Helicobacter pylori , can promote cell growth and migration of gastric cancer [ 40 ]. THAP9-AS1 can also promote pancreatic ductal adenocarcinoma growth and lead to a poor clinical outcome via sponging miR-484 and interacting with YAP [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA THAP9‐AS1 and TSPOAP1‐AS1 Provide Potential Diagnostic Signatures for Pediatric Septic Shock

Yin

Zhang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Background. Sepsis is a systemic inflammatory syndrome caused by infection with a high incidence and mortality. Although long noncoding RNAs have been identified to be closely involved in many inflammatory diseases, little is known about the role of lncRNAs in pediatric septic shock. Methods. We downloaded the mRNA profiles GSE13904 and GSE4607, of which GSE13904 includes 106 blood samples of pediatric patients with septic shock and 18 health control samples; GSE4607 includes 69 blood samples of pediatric patients with septic shock and 15 health control samples. The differentially expressed lncRNAs were identified through the limma R package; meanwhile, GO terms and KEGG pathway enrichment analysis was performed via the clusterProfiler R package. The protein-protein interaction (PPI) network was constructed based on the STRING database using the targets of differently expressed lncRNAs. The MCODE plug-in of Cytoscape was used to screen significant clustering modules composed of key genes. Finally, stepwise regression analysis was performed to screen the optimal lncRNAs and construct the logistic regression model, and the ROC curve was applied to evaluate the accuracy of the model. Results. A total of 13 lncRNAs which simultaneously exhibited significant differences in the septic shock group compared with the control group from two sets were identified. According to the 18 targets of differentially expressed lncRNAs, we identified some inflammatory and immune response-related pathways. In addition, several target mRNAs were predicted to be potentially involved in the occurrence of septic shock. The logistic regression model constructed based on two optimal lncRNAs THAP9-AS1 and TSPOAP1-AS1 could efficiently separate samples with septic shock from normal controls. Conclusion. In summary, a predictive model based on the lncRNAs THAP9-AS1 and TSPOAP1-AS1 provided novel lightings on diagnostic research of septic shock.

show abstract

“…4 Reportedly, lncRNAs can markedly regulate the progression of multiple cancers including GC. [5][6][7] For instance, as an oncogenic lncRNA, lncRNA AFDN-DT can inhibit GC cell growth through transcriptional regulation; 8 as a tumorsuppressive factor, LINC00460 is significantly upregulated in GC tissues, and promotes the malignant biological behaviors of cancer cells via epigenetically silencing CCNG2. 9 LncRNA C-terminal binding protein 1 antisense RNA 2 (CTBP1-AS2) is not only related to the susceptibility to type 2 diabetes 10 but also closely connected with the recurrence of papillary thyroid cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis</p>

Yang

Gao

Liu

et al. 2020

OTT

View full text Add to dashboard Cite

Background This study aimed at probing into the effect of long non-coding RNA (lncRNA) C-terminal binding protein 1 antisense RNA 2 (CTBP1-AS2) on gastric cancer (GC) cell proliferation and apoptosis, and its regulatory function on miR-139-3p and MMP11 . Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to examine the expressions of CTBP1-AS2, miR-139-3p and MMP11 mRNA in GC cell lines and clinical specimens. Cell counting kit-8 (CCK-8) assay, flow cytometry and EdU assay were conducted to examine the effects of CTBP1-AS2 and miR-139-3p on GC cell proliferation and apoptosis. Western blot was applied for detecting the expressions of Bax, Bcl-2 and MMP11 . A lung metastasis mouse model was used to evaluate metastasis of GC cells in vivo. Bioinformatics, dual-luciferase report assay, RIP and RNA pull-down assays were utilized to validate the targeted relationship between CTBP1-AS2 and miR-139-3p as well as the targeting relationship between miR-139-3p and MMP11 . Results CTBP1-AS2 was highly expressed in GC, and its high expression was strongly associated with increased TNM stage, increased tumor size and low degree of differentiation of the tumor tissues. Meanwhile, CTBP1-AS2 promoted GC cell proliferation, metastasis and suppressed apoptosis, while miR-139-3p could weaken these effects. In addition, CTBP1-AS2 was identified as a molecular sponge for miR-139-3p, and MMP11 was verified as a target gene of CTBP1-AS2. CTBP1-AS2 could increase the expression of MMP11 via repressing miR-139-3p. Conclusion CTBP1-AS2 promotes GC cells and inhibits apoptosis by regulating the miR-139-3p/ MMP11 molecular axis.

show abstract

<p>Long noncoding RNA THAP9-AS1 is induced by <em>Helicobacter pylori</em> and promotes cell growth and migration of gastric cancer</p>

Cited by 27 publications

References 43 publications

Stress induced Differential Expression of THAP9 & THAP9-AS1 in the S-phase of cell cycle

Stress induced Differential Expression of THAP9 & THAP9-AS1 in the S-phase of cell cycle

Long Noncoding RNA THAP9‐AS1 and TSPOAP1‐AS1 Provide Potential Diagnostic Signatures for Pediatric Septic Shock

<p>LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis</p>

Contact Info

Product

Resources

About