<p>Oncolytic Vaccinia Virus-Mediated Antitumor Effect and Cell Proliferation Were Promoted in PTC by Regulating circRNA_103598/miR-23a-3p/IL-6 Axis</p>

Abstract

Background: Papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy, and cases have been rising steadily worldwide in the past few decades. Despite great progress having been made in surgery and chemotherapy for PTC, the survival rate of PTC patients has not increased significantly. Therefore, there is an urgent need to explore novel treatment strategies. Materials and Methods: The levels of circRNA_103598, miR-23a-3p and IL-6 mRNA in PTC tissues and cells were examined by qRT-PCR assay. Cell proliferation and IC 50 values of oncolytic vaccinia virus (OVV) were detected by CCK-8 assay. A dual-luciferase reporter assay was performed to detect the relationships among circRNA_103598, miR-23a-3p and IL-6. ELISA was carried out to detect the expression of IL-6. Results: We found that circRNA_103598 was increased in PTC tissues and cell lines and acted as a sponge for miR-23a-3p. Moreover, knockdown of miR-23a-3p suppressed the OVV-mediated antitumor effect and cell proliferation in PTC. In addition, we revealed that circRNA_103598 bound to miR-23a-3p as a sponge to promote IL-6 expression. Conclusion: Our study first revealed the high expression and oncogenic function of circRNA_103598 in PTC cells. Then, circRNA_103598 sponged miR-23a-3p to upregulate IL-6 expression, with the resulted that cell proliferation was promoted and the OVVmediated antitumor effect was enhanced by strengthening the viral replication, providing new insights into future therapy for PTC.

Emerging Roles of Circular RNAs in Thyroid Cancer

“…Similarly, circEIF6 knockdown effectively reduced tumor weight in the nude mice treating with cisplatin, while circEIF6 knockdown alone had no effect on tumor weight ( Liu et al, 2018 ). Moreover, Zhang S. et al (2020) revealed that circRNA_103598 increased as a regulator increasing the expression of IL-6 via sponging miR-23a-3p in PTC, with promoted cell proliferation and the enhanced oncolytic vaccinia virus (OVV)-mediated antitumor effect by strengthening the viral replication.…”

Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

“…As the results, the new focus is on targeting the immune response, rather than the tumor itself [12]. A series of studies have shown that vaccinia virus can not only directly lyse tumor cells [13,14], but also initiate the anti-tumor immune responses [15][16][17]. On the other hand, several studies indicated that C-type lectin could enhance the immune response [18,19].…”