<p>Research on the establishment of a TPM3 monoclonal stable transfected PANC-1 cell line and the experiment of the EMT occurrence in human pancreatic cancer</p>

Abstract

Background: Pancreatic cancer is one of the most aggressive human malignancies that is associated with early metastasis and chemoresistance. Tropomyosin (TPM) is an indispensable regulatory protein for muscle contraction, Abnormal expressions of TPM gene are closely related to the carcinogenesis and metastasis of malignant tumors. Purpose: In this experiment, a monoclonal stable transfected cell line was established by the knock-down of TMP3 expression in PANC-1 cells with the lentivirus method, and the impacts of the downregulated TPM3 gene expression on the EMT-related molecules and biological behaviors of PANC-1 cells were explored. Methods: Based on the TPM3 gene sequence, we designed the RNA interference sequence, constructed and screened out the recombinant plasmid segment TPM3-shRNA with the optimal silencing effect, and carried out lentivirus titer determination and packaging. The recombinant lentiviral interference vector LV-TPM3-shRNA was transfected into PANC-1 cells; the transfection efficiency was then evaluated to screen out the monoclonal stable transfected PANC-1 cell line with downregulated TPM3 expression. The qRT-PCR and Western blot were used to detect the changes in the gene- and protein-levels expressions of EMT-related transcription factors in the target cell line and to respectively test the variations of the invasion and proliferation capacities. Results: It is shown that the monoclonal stable transfected PANC-1 cell line with downregulated TPM3 expression was successfully established with the recombinant lentiviral vector. After knocking down the expression of TPM3 gene in PANC-1 cells, EMT occurred in the cells; the cell phenotype showed malignant transformation, and the in vitro biological behaviors of the cells (such as proliferation and invasion) were enhanced to different degrees. Conclusion: It is indicated that the TPM3 gene can be a potential target spot for the treatment of pancreatic cancer.

Systematic Analysis of an Invasion-Related 3-Gene Signature and Its Validation as a Prognostic Model for Pancreatic Cancer

“…Pancreatic adenocarcinoma (PAAD) is a malignant tumor of the digestive tract and is the fourth leading cause of cancer-related deaths worldwide ( 1 ). Since the early symptoms of PAAD are not obvious, its diagnosis is often difficult, and the primary tumor exhibits vascular invasion.…”

<p>DEP Domain-Containing Protein 1B (DEPDC1B) Promotes Migration and Invasion in Pancreatic Cancer Through the Rac1/PAK1-LIMK1-Cofilin1 Signaling Pathway</p>

“…According to population-based cancer registration data, pancreatic cancer (PC) is one of the most malignant digestive tract tumors, ranking as the fourth leading cause of death among all cancers. [1][2][3] Because the early symptoms of PC are obscure and because PC is prone to vascular invasion, 80-85% of patients have lost the chance for radical resection at diagnosis. 4 Surgical resection is the only potential cure for PC.…”

PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma

“…PCBP1 knockdown simultaneously accompanied a decrease in TPM3 expression and inhibited migration and invasion of ESCC cells. Although TPM3 plays a complex role in a variety of human tumors [ 18 , 34 ], to our knowledge, this is the first study to confirm post-transcriptional regulation of TPM3 mRNA mediated by RNA-binding proteins in esophageal squamous cell carcinoma.…”