&lt;title&gt;In-vivo confocal microscopy based on the Texas Instruments digital micromirror device&lt;/title&gt;

Botvinick, Elliott L.; Li, Florence; Cha, Sungdo; Gough, David; Fainman, Yeshaiahu; Price, Jeffrey H.

doi:10.1117/12.384208

Cited by 6 publications

(2 citation statements)

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“…Another conceivable approach for scanning a large field of view is by means of parallel laser scanning microscopy, that is, multiple‐spot scanning (as in spinning‐disk confocals or LaVision's TRIMSCOPE 64‐spot multiplexer for scanning microscopes). The use of digital micromirror devices offers an alternative via large arrays of rapidly reconfigurable micromirrors (Botvinick et al , 2000). The same principle is readily extended to fast wavelength multiplexing of a white‐light supercontinuum (McConnell et al , 2006), offering even more flexibility without moving parts.…”

Section: Multiplexed Imaging With Multiple‐beam Microscopesmentioning

confidence: 99%

High‐throughput microscopy must re‐invent the microscope rather than speed up its functions

Oheim

2007

British J Pharmacology

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Knowledge gained from the revolutions in genomics and proteomics has helped to identify many of the key molecules involved in cellular signalling. Researchers, both in academia and in the pharmaceutical industry, now screen, at a sub-cellular level, where and when these proteins interact. Fluorescence imaging and molecular labelling combine to provide a powerful tool for real-time functional biochemistry with molecular resolution. However, they traditionally have been work-intensive, required trained personnel, and suffered from low through-put due to sample preparation, loading and handling. The need for speeding up microscopy is apparent from the tremendous complexity of cellular signalling pathways, the inherent biological variability, as well as the possibility that the same molecule plays different roles in different sub-cellular compartments. Research institutes and companies have teamed up to develop imaging cytometers of ever-increasing complexity. However, to truly go high-speed, sub-cellular imaging must free itself from the rigid framework of current microscopes. Keywords: fluorescence microscopy; quantitative imaging; subcellular localization; pattern recognition; tissue microarrays; cellular diagnostics; protein activity Abbreviations: DFC, dielectric field cage; DMD, digital micromirror device; DSI, dynamic speckle illumination; FRET, fluorescence resonance energy transfer; HTM, high-throughput microscopy; OFM, optofluidic microscopy; P-LSM, parallel laser scanning microscopy; TIRF, total internal reflection fluorescenceEarly microscopy is above all a history of instrument development. Skilled lens grinding, improved mechanical stability and careful matching of lenses together led to the building of 17th-century telescopes and microscopes. Botanists, physicians and anatomists began to draw, discuss and publish their observations in luxurious book editions that increasingly attracted a growing scientifically interested public. The manufacture of less expensive microscopes during Victorian times allowed far more people to see the world of microscopic organisms in a drop of pond water or to observe the formerly unimagined intricate details of the structure of animals, plants and crystals. Exhibitions or 'conversazione' were organized where many microscopes stood side by side to demonstrate the wonders of the microscopic world. Despite impressive progress throughout the 19th and early 20th century, it is surprising how little microscopy has changed. A look into many of today's imaging facilities stunningly resembles the 19th-century engraving in Figure 1. All the elements are already there: microscopists preparing samples, gazing through the eyepieces of bulky microscopes, capturing images of a fairly small number of cells and excitedly sharing images. Certainly, these early microscopes have little to rival the resolving power, sensitivity and variety of different contrast modes offered by modern research instruments. Digital imaging has revolutionized image acquisition, processing and data handling, a...

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Section: Multiplexed Imaging With Multiple‐beam Microscopesmentioning

confidence: 99%

High‐throughput microscopy must re‐invent the microscope rather than speed up its functions

Oheim

2007

British J Pharmacology

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“…MOEMS scanning mirrors have enjoyed great commercial success in the field of display. The most famous example is that of Texas Instruments’ digital micromirror devices [ 14 ], which are widely used in projectors. Combining QPD and MOEMS, 2D scanning mirrors with embedded sensors have been built, modeled, and analyzed [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Optoelectronic Angular Displacement Measurement Technology for 2-Dimensional Mirror Galvanometer

Hung

Chung

Chen

et al. 2022

Sensors

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The mirror galvanometer is a crucial component of laser cutters/engravers. Novel two-dimensional mirror galvanometers demonstrate less trajectory distortion than traditional one-dimensional ones. This article proposes an optoelectronic sensor that measures a mirror’s inclinations in two dimensions simultaneously. The measuring range, resolution, and sampling rate are ±10°, 0.0265°, and 2 kHz, respectively. With the proposed sensor, a closed-loop control can be further implemented to achieve precision laser machining. Its compact size and low cost meet the requirements of miniature laser engravers, which have become popular in recent years.

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Parallel large-range scanning confocal microscope based on a digital micromirror device

Zhang

Strube

Molnár

et al. 2013

Optik

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<title>In-vivo confocal microscopy based on the Texas Instruments digital micromirror device</title>

Cited by 6 publications

References 6 publications

High‐throughput microscopy must re‐invent the microscope rather than speed up its functions

High‐throughput microscopy must re‐invent the microscope rather than speed up its functions

Optoelectronic Angular Displacement Measurement Technology for 2-Dimensional Mirror Galvanometer

Parallel large-range scanning confocal microscope based on a digital micromirror device

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