Auto-stereoscopic display uses LCD odd column pixels as left eye’s view image display and even column pixels as right eye’s,its can be perceived stereo vision by using special optic element to alter the light way of display and eye’s image system. The display panel’s optic structure contains on-off marrow slot illumination panel, TFT-LCD cell and backlight module. In this paper, We put forward an assemble method called dynamical assemble for 3D TFT LCD panel which used in our research work, .and analyzed its effect.