Lung fixation by perfusion—a simple method to control the pressure in the perfusion circuit

Abstract

SUMMARY A simple method which maintains the hydraulic pressure at a constant level during fixation of organs by perfusion is described. This is obtained by means of a glass tube interposed between a peristaltic pump and the organ to be perfused, the specially designed glass tube acting as a safety valve. The efficiency of the system is tested by electromagnetic pressure recording.

"Contractile Interstitial Cells" in Pulmonary Alveolar Septa: A Possible Regulator of Ventilation/Perfusion Ratio?

“…The lungs of 20 Wistar rats (150 g) deeply anesthetized by intraperitoneal (i .p .) Nembutal (17 mg/100 g) were fixed by perfusion (10)(11)(12) ; 6 other rat lungs were fixed by conventional intratracheal instillation. We used 2% glutaraldehyde adjusted with phosphate buffer and saccharose to pH 7 .4 and 350 mosmol .…”

A simple method of lung perfusion fixation

“…During the past few years, elegant studies which correlate pulmonary structural configurations with known functional conditions have been published by the Swiss scientists (Assimacopou-10s et al, 1976;Bachofen et al, 1979;Gil et al, 1979;Gil and Weibel, 1971). However, a disadvantage for many workers in lung research in adopting their techniques is the requirement for rather sophisticated instrumentation and expensive equipment (Assimacopoulos and Kapanci, 1973;Gil and Weibel, 1971). The use of air from a n air inlet valve, which should be available in any laboratory, obviates the need for a peristaltic pump for perfusate delivery.…”

Effects of Metabolite VIII of Bromexine (Na 872) on Type II Epithelium of the Lung

“…The lungs were fixed in situ by perfusion ac cording to the method described elsewhere [2). The perfusion pressure was adjusted to 35 cm H20 and the intratracheal pressure to 12 cm H ,0.…”