Lung injury caused by paraquat poisoning results in increased interleukin‑6 and decreased microRNA‑146a levels

Wu, Wei; Yong, Li

doi:10.3892/etm.2018.6153

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…A number of genes that are differentially expressed during the occurrence and development of inflammation are candidate targets for gene therapy, including some microRNAs (miRNAs/miRs) (10). A recent study reported that miR-146a can regulate the occurrence and immune response of lung injury induced by paraquat poisoning, and that interleukin (IL)-6 is involved in the regulation of this process (11).…”

Section: Introductionmentioning

confidence: 99%

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Dai

Zhang

et al. 2020

Exp Ther Med

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The aims of the present study were to examine the clinical significance of serum microRNA-27a (miR-27a) levels in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat poisoning and to investigate the correlation between miR-27a and interleukin (IL)-10. A total of 82 patients with MODS induced by acute paraquat poisoning and 88 healthy controls were recruited in the present study. Reverse transcription-quantitative PCR was used to measure serum miR-27a expression levels in patients with MODS and the control group. IL-10 serum levels were determined using ELISA. Decreased serum miR-27a level and increased IL-10 expression levels were detected in patients with paraquat poisoning compared with healthy controls (P<0.001). A moderately negative correlation was identified between the serum expression levels of miR-27a and IL-10 (r=-0.5225; P<0.001). miR-27a expression level was found to be associated with blood urea nitrogen, partial pressure of carbon dioxide, arterial blood lactic acid, and the acute physiology and chronic health evaluation II score (APACHE II; P<0.05). The area under the curve for miR-27a was 0.946, with a sensitivity of 86.6% and specificity of 87.5% at a cutoff value of 2.10. The non-survival patient group had lower miR-27a expression levels compared with the survival group (P<0.01). Multivariate Cox regression analyses suggested miR-27a expression level and APACHE II score were independent prognostic factors for 30-day mortality (P<0.01). The present results suggested that serum miR-27a level may be a potential novel diagnostic and prognostic factor for MODS caused by paraquat poisoning. Collectively, miR-27a may be involved in the process of MODS induced by paraquat poisoning by regulating the inflammatory response.

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Section: Introductionmentioning

confidence: 99%

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Dai

Zhang

et al. 2020

Exp Ther Med

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“…13 Additionally, miR-146a might be involved in the occurrence of lung injury induced by PQ poisoning, which was achieved via IL-6. 14 Toll like receptor 4 (TLR4) is a pattern recognition receptor, which is a vital part of the innate immune system and has the regulatory effects on the acute inflammatory response. 15 Liu et al 16 found that the mRNA level of TLR4 markedly increased in lung tissue treated by PQ, consequently TLR4 might play a vital role in PQ-induced acute lung injury.…”

Section: Introductionmentioning

confidence: 99%

“…13 Additionally, miR-146a might be involved in the occurrence of lung injury induced by PQ poisoning, which was achieved via IL-6. 14…”

Section: Introductionmentioning

confidence: 99%

Aberrant miR-219-5p is correlated with TLR4 and serves as a novel biomarker in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Dai

Liu

Guo

2020

Int J Immunopathol Pharmacol

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This study aimed to investigate the clinical significance of serum microRNA-219-5p (miR-219-5p) in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat (PQ) poisoning, and its correlation with Toll-like Receptor 4 (TLR4). Luciferase reporter assay was used to investigate in vitro the correlation of miR-219-5p with TLR4. Serum miR-219-5p levels were evaluated by quantitative real-time polymerase chain reaction. Serum levels of TLR4, IL-1β, and TNF-α were measured by Enzyme-linked immune sorbent assay (ELISA). ROC analysis was performed to assess the diagnostic significance, Kaplan-Meier survival curves and Cox regression analysis were used to evaluate the prognostic value of miR-219-5p in MODS patients. TLR4 was a target gene of miR-219-5p and was increased in MODS patients. Serum miR-219-5p level was decreased and negatively correlated with TLR4 level in MODS patients ( r = −0.660, P < 0.001), which had important diagnostic value and negatively correlated with APACHE II score in MODS patients. The miR-219-5p expression was markedly associated with the WBC, ALT, AST, PaCO2, Lac, and APACHE II score. Non-survivals had more patients with low miR-219-5p expression. Patients with low miR-219-5p expression had shorter survival time. MiR-219-5p and APACHE II score were two independently prognostic factors for 28-day survival. MiR-219-5p was negatively correlated with, while TLR4 was positively correlated with the levels of IL-1β and TNF-α. The serum miR-219-5p level may be a potential biomarker for acute PQ-induced MODS diagnosis and prognosis. Furthermore, miR-219-5p may be associated with the progression of MODS by regulating TLR4-related inflammatory response.

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“…Based on experiments using pulmonary macrophages, peripheral blood mononuclear cells and serum from patients with lung injury caused by Paraquat poisoning, a dual-luciferase reporter assay demonstrated that the IL-6 mRNA is a direct target of miR-146a. Accordingly, it was suggested that increased expression of IL-6 in patients with lung injury caused by Paraquat poisoning was associated with decreased expression of miR-146a (Table I) (15). Furthermore, studies performed using human papillomavirus (HPV)16 E6/E7-positive keratinocytes identified the histone demethylase KDM2B as a new direct target of miR-146a, and two putative binding sites for miR-146a were identified in its 3′-UTR sequence (Table I) (16).…”

Section: Introduction: Micrornas (Mirnas/mirs) and Mir-146amentioning

confidence: 99%

Multiple roles of microRNA‑146a in immune responses and hepatocellular carcinoma (Review)

Wang

et al. 2019

Oncol Lett

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MicroRNAs (miRNAs/miRs), consisting of ~22 nucleotides of single-stranded RNA, participate in post-transcriptional gene regulation by binding to the 3′-untranslated region (UTR) of mRNAs, repressing their translation and promoting their degradation. Studies have shown that certain miRNAs play a key role in the control of various cellular activities, such as inhibiting inflammation, modulating cell differentiation and suppressing cancer growth. The role of miR-146a in the immune response and in the pathogenesis of hepatocellular carcinoma (HCC) has also been investigated. Although some studies have shown that increased miR-146a levels are associated with HCC, others have revealed that miR-146a suppresses cancer cell proliferation, invasion and metastasis. Toll-like receptor 4 (TLR4) signaling has an important role in regulating innate and adaptive immune responses. In addition, TLR4 is functionally expressed in HCC cells and promotes HCC cell proliferation, which can be regulated by miR-146a. The present review focuses on the recent progress in analyzing the multiple roles of miR-146a in mediating the TLR4 pathway and adaptive immune response. Finally, the function of miR-146a in the pathogenesis of HCC is also discussed.

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Lung injury caused by paraquat poisoning results in increased interleukin‑6 and decreased microRNA‑146a levels

Cited by 8 publications

References 28 publications

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Aberrant miR-219-5p is correlated with TLR4 and serves as a novel biomarker in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Multiple roles of microRNA‑146a in immune responses and hepatocellular carcinoma (Review)

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