Lung tissue mechanics and extracellular matrix composition in a murine model of silicosis

Faffe, Débora S.; Silva, Gabriela H.; Kurtz, Pedro M.; Negri, Eva; Capelozzi, Vera Luíza; Rocco, Patricia R. M.; Zin, Walter A.

doi:10.1152/jappl.2001.90.4.1400

Cited by 57 publications

(72 citation statements)

References 27 publications

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“…The changes observed in DP1,L could be attributed to the intrabronchial cellular infiltration obstructing the lumen. The increase in Est,L and DP2,L pressure may be associated with the presence of granulomatous nodules, increased number of cells in the alveolar septa and granuloma, alveolar collapse, distortion of patent alveoli and interstitial oedema in accordance with previous studies on silica-treated BALB/c mice [14][15][16]. The SIL group also showed an increase in the number of macrophages in the granuloma and lung parenchyma, which may release inflammatory cytokines such as IL-1 [17], yielding a recruitment of inflammatory cells into the alveolar septa [17,18].…”

Section: Lung Cell Biology T Maron-gutierrez Et Alsupporting

confidence: 89%

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Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis

Maron‐Gutierrez¹,

Castiglione²,

Xisto³

et al. 2010

Eur Respir J

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This study tests the hypothesis that bone marrow-derived mononuclear cell (BMDMC) therapy may reduce lung inflammation and fibrosis leading to an improvement in respiratory mechanics in a murine model of silicosis.52 female C57BL/6 mice were randomly assigned into four groups. In the silica group (SIL), silica suspension (20 mg/50 mL in saline) was intratracheally instilled. In the control animals, 50 mL saline was administered intratracheally. At 1 h, the control and SIL groups were further randomised, receiving BMDMC (2610 6 In the SIL-cell group, the fraction area of granuloma, the number of macrophages and the collagen fibre content were reduced, yielding improved lung mechanics. The presence of male donor cells in lung tissue was not confirmed using detection of Y chromosome DNA. Nevertheless, caspase-3, IL-1b, IL-1a, IL-1RN and TGF-b mRNA expression diminished after cell therapy.In conclusion, BMDMC acted on inflammatory and fibrogenic processes improving lung function through paracrine effects.

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Section: Lung Cell Biology T Maron-gutierrez Et Alsupporting

confidence: 89%

“…changes at day 15 mimicking the clinical setting [14,15]. Silica animals presented increased Est,L, DP1,L and DP2,L pressures.…”

Section: Lung Cell Biology T Maron-gutierrez Et Almentioning

confidence: 86%

Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis

Maron‐Gutierrez¹,

Castiglione²,

Xisto³

et al. 2010

Eur Respir J

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“…Although human disease typically progresses over many years (even decades), pulmonary inflammation in this model system develops rapidly and resolves over the course of several weeks. Nevertheless, this murine model shares important characteristics with human silicosis, including acute neutrophilic extravasation, increased protein in BAL, and progressive fibrosis (21,23,24). Results from this study establish the utility of suppressive ODN in the prevention of acute pulmonary inflammation, and identify the cell types and mechanism associated with this protection.…”

mentioning

confidence: 71%

Suppressive Oligodeoxynucleotides Inhibit Silica-Induced Pulmonary Inflammation

Sato¹,

Shimosato²,

Alvord

et al. 2008

The Journal of Immunology

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Inhalation of silica-containing dust particles induces silicosis, an inflammatory disease of the lungs characterized by the infiltration of macrophages and neutrophils into the lungs and the production of proinflammatory cytokines, chemokines, and reactive oxygen species (ROS). Synthetic oligodeoxynucleotides (ODN) expressing “immunosuppressive motifs” were recently shown to block pathologic inflammatory reactions in murine models of autoimmune disease. Based on those findings, the potential of suppressive ODN to prevent acute murine silicosis was examined. In vitro studies indicate that suppressive ODN blunt silica-induced macrophage toxicity. This effect was associated with a reduction in ROS production and p47phox expression (a subunit of NADPH oxidase key to ROS generation). In vivo studies show that pretreatment with suppressive (but not control) ODN reduces silica-dependent pulmonary inflammation, as manifest by fewer infiltrating cells, less cytokine/chemokine production, and lower levels of ROS (p < 0.01 for all parameters). Treatment with suppressive ODN also reduced disease severity and improved the survival (p < 0.05) of mice exposed to silica.

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“…where DF is the total change in force before and after strip immersion in K-H solution and d is the mass density of the K-H solution [21][22][23]. One end of the material was attached to a force transducer (FT03; Grass Instruments Co., Quincy, MA, USA), while the other end was fastened to a vertical rod.…”

Section: Measurement Of Tissue Mechanicsmentioning

confidence: 99%

“…Neither amplitude dependence (v0.1% change in stiffness) nor phase changes with frequency were detected in the range of 0.01-14 Hz. The hysteresivity of the system was independent of frequency and had a value v0.003 [22,23].…”

Section: Measurement Of Tissue Mechanicsmentioning

confidence: 99%

Therapeutic potential of a new phosphodiesterase inhibitor in acute lung injury

Rocco¹,

Momesso²,

Figueira³

et al. 2003

Eur Respir J

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The effects of LASSBio596, a phosphodiesterase type-4 and -5 inhibitor, were tested in Escherichia coli lipopolysaccharide (LPS)-induced acute lung injury.Twenty-four BALB/c mice were randomly divided into four groups. In the control group, saline (0.05 mL) was injected intratracheally (i.t.). The LPS group received LPS (10 mg i.t., 0.05 mL). In the LASSBio596 groups, LASSBio596 (10 mg?kg -1 , 0.2 mL) was injected intraperitoneally 1 h before or 6 h after LPS administration. After 24 h, in vivo (lung resistive and viscoelastic pressures, and static and dynamic elastances) and in vitro (tissue resistance, elastance and hysteresivity) pulmonary mechanics, lung morphometry and collagenous fibre content were computed. Neutrophils and tumour necrosis factor (TNF)-a levels were evaluated in the bronchoalveolar lavage fluid.LASSBio596 prevented the changes in lung mechanics, and inhibited neutrophilic recruitment, TNF-a release, bronchoconstriction, alveolar collapse and the increment of collagen fibre content induced by LPS, independently of the moment of injection.In conclusion, LASSBio596 modulated the lung inflammatory process and had the potential to block fibroproliferation. Thus, agents that inhibit phosphodiesterase 4 and 5 simultaneously may be a useful adjunct therapy for acute lung injury.

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Lung tissue mechanics and extracellular matrix composition in a murine model of silicosis

Cited by 57 publications

References 27 publications

Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis

Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis

Suppressive Oligodeoxynucleotides Inhibit Silica-Induced Pulmonary Inflammation

Therapeutic potential of a new phosphodiesterase inhibitor in acute lung injury

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