Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium

Abstract

A novel Gram-stain-positive actinobacterium, designated RI148-Li105 T , was isolated from a lichen sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. 16S rRNA gene sequencing study indicated that strain RI148-Li105 T was related to the type strain of Luteimicrobium subarcticum, with a similarity of 97.8%. Cells of strain RI148-Li105 T exhibited a rod-coccus cycle. The diagnostic cell-wall diamino acid of this organism was lysine and the peptidoglycan type was found to be A4a. The predominant menaquinones were MK-8(H 2 ) and MK-9(H 2 ), and the major fatty acids were iso-C 16:0 , C 17:1 x9c and C 17:0 . The DNA G þ C content was 73.6 mol%. The major phenotypic characteristics of strain RI148-Li105 T basically corresponded to those of the genus Luteimicrobium excluding the fatty acid composition. These results suggest that strain RI148-Li105 T should be affiliated with the genus Luteimicrobium. Meanwhile, DNA-DNA hybridization and some phenotypic characteristics revealed that the strain differs from L. subarcticum. Therefore, strain RI148-Li105 T represents a novel species of the genus Luteimicrobium, for which the name Luteimicrobium album sp. nov. is proposed. The type strain of Luteimicrobium album is RI148-Li105 T ( ¼ NBRC 106348 T ¼ DSM 24866 T ).

Janibacter cremeus sp. nov., an actinobacterium isolated from sea sediment

“…Growth at 5 and 10 u C was also evaluated after 14 days of incubation. Cell motility, pH range and NaCl tolerance for growth, anaerobic growth, Gram staining and oxidase and catalase activities were determined using the methods outlined by Hamada et al (2012a). Other physiological and biochemical tests were performed using API ZYM, API Coryne and API 50CH systems (bioMérieux) according to the manufacturer's instructions.…”

Draft genome sequence of Streptomyces sp. TP-A0867, an alchivemycin producer

“…These results are summarized in Table 1. The genes encoding 16S rRNA were amplified by PCR using two universal primers, 9 F (5′-GAGTTTGATCCTGGCTCAG-3′) and 1541R (5′-AAGGAGGTGATCCAGCC-3′) [7]. KOD FX (Toyobo Co., Ltd., Tokyo, Japan) was used as described by the manufacturer for the PCR.…”

“…The amplicon size was 1.5 kb. After purification of the PCR product by AMPure (Beckman Coulter), sequencing was carried out according to an established method [7]. The sequence was deposited into DDBJ under the accession number LC150789.…”

Actinoplanes bogoriensis sp. nov., a novel actinomycete isolated from leaf litter

“…Analyses of sugar composition in whole-cell hydrolysate, amino acids and their isomers in cell-wall hydrolysate and isoprenoid quinones were determined according to the methods described by Hamada et al 15 Polar lipids were investigated by the method of Minnikin et al 16 Cellular fatty acid methyl esters were prepared and analyzed following the standard protocol of MIDI Sherlock Microbial Identification System 17 using ACTINO database (version 4.0; MIDI, Newark, DE, USA) and detected using GC (model 6890N; Agilent Technologies, Santa Clara, CA, USA).…”