Lycium barbarum Polysaccharides Attenuate Cisplatin-Induced Hair Cell Loss in Rat Cochlear Organotypic Cultures

Abstract

The aim of the present study was to investigate the effects of Lycium barbarum polysaccharides (LBP) on cisplatin-induced hair cell damage in the organ of Corti explant. The neonatal (P2–3) rat organ of Corti explant was exposed to cisplatin (20 μM; 48 h) with or without LBP pretreatment (150 and 600 μg/mL; 24 h). Hair cell loss was indicated by FITC-labeled phalloidin staining. The level of reactive oxygen species (ROS) and alteration of mitochondrial membrane potential (ΔΨm) in hair cells were analyzed using fluorescent probes 2′,7′-dichlorofluorescein diacetate and JC-1, respectively. The results showed that LBP significantly attenuated hair cell loss (p < 0.01). Hair cells pretreated with LBP showed significant reduction in ROS production and the decline of ΔΨm compared with cisplatin alone group (p < 0.01), indicating the protective effect of LBP on cisplatin-induced hair cell loss. Taken together, these results indicate that LBP was effective in attenuating cisplatin-induced hair cell loss by reducing the production of ROS and maintaining mitochondrial ΔΨm.

Lycium barbarum Polysaccharides Protect Human Lens Epithelial Cells against Oxidative Stress–Induced Apoptosis and Senescence

“…Lycium barbarum is a well-known traditional Chinese herbal medicine that has multiple pharmacological and biological functions, including neuroprotection [9] – [12] , antioxidant properties [13] – [15] , anti-aging properties [16] , [17] , cytoprotection [18] , [19] , and immuno-modulating properties [14] , [20] . L. barbarum polysaccharides (LBPs) extracted from L. barbarum fruits, are believed to be the main component responsible for these biological activities [21] .…”

Charge transfer regulated by domain differences between host and guest donors in ternary organic solar cells

“…In addition, P3HT, DT and PC 71 BM could form a gradient distribution of energy levels in turn. 24 Therefore, we speculate that P3HT with relatively strong electron-giving characteristics is more suitable as the host donor in the DT:P3HT:PC 71 BM, where a small amount of DT needs to be added, that is DT:P3HT(0.9):PC 71 BM. Meanwhile in DT:DTE:PC 71 BM, DTE is more suitable as a guest donor obtaining DT:DTE(0.1):PC 71 BM due to the introduction of ester groups with a larger steric hindrance.…”

“…However, in our previous work, it was proved that the related mechanisms in the P3HT:SMPV1:PC 71 BM and DR3TBDTT:DR3TBDTT-E:PC 71 BM systems are different. 24 It is foreseeable that there are many screening criteria for the third component to improve PCE, [25][26][27][28] since the third component involved in different photophysical processes. 29,30 However, with the development of PCE, various mechanisms that can coexist in T-OSCs have been derived from the perspective of energetics and thermodynamics, which means that the introduction of the third component complicates the microstructure of T-OSCs, making it difficult to improve PCE.…”

Non-fullerene acceptor alloy strategy enabling stable ternary polymer solar cells with efficiency of 17.74%

“…[5][6][7][8][9][10] To further enhance the performance of the devices in terms of efficiency and stability, the ternary strategy of preparing ternary organic solar cells (TOSCs) by introducing an extra donor or acceptor into the host binary system has been widely used and proved to be reasonable and effective. [11][12][13] According to the different intermolecular interactions among the three components in the blend system, the mechanism of performance enhancement of devices can be described by the following models: parallel, 14,15 charge transfer, 16,17 energy transfer 18,19 and alloy models, [20][21][22] and there are often more than two model mechanisms that work together in a TOSC, 23,24 which presents challenges in the choice of the third components. This is not only because it is necessary to carefully choose the appropriate mechanism model to improve the performance of a certain material system, but more importantly, the random introduction of the third component may destroy the charge transport channels and fine film morphology of the original binary system.…”