Lymphatic Tissue Bioengineering for the Treatment of Postsurgical Lymphedema

Sung, Cynthia; Gupta, Kapil; Wang, Jin; Wong, Alex K.

doi:10.3390/bioengineering9040162

Cited by 8 publications

(7 citation statements)

References 137 publications

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“…However, collagen deposition and vascular structures were observed around the hADSC injection site, and structures such as lymph node capsules were identified. Previous investigators have reported that hADSCs injection improved lymphedema in an animal model of lymphedema 7,20 . In our results, the effect of hADSCs injection was not significant.…”

Section: Discussionmentioning

confidence: 96%

Recellularized lymph node scaffolds with human adipose-derived stem cells enhance lymph node regeneration to improve lymphedema

Kang

Moon

Kim

et al. 2023

Sci Rep

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To overcome the limitations of lymphedema treatment, human adipose-derived stem cells (hADSCs) were injected into decellularized lymph nodes to produce a recellularized lymph node-scaffold, and the effect of lymphangiogenesis was investigated in lymphedema animal models. Axillary lymph nodes were harvested from Sprague Dawley rats (7 weeks old, 220–250 g) for decellularization. The decellularized lymph nodes were performed and PKH26-labeled hADSCs (1 × 106/50 µL) were injected in the decellularized lymph node-scaffolds. Forty rats were equally divided into four groups: lymphedema as control group, hADSC group, decellularized lymph node-scaffold group, and recellularized lymph node-scaffold group. The lymphedema model was made by removing inguinal lymph nodes, and hADSCs or scaffolds were transplanted. Histopathological assessments were performed by hematoxylin and eosin and Masson’s trichrome staining. Lymphangiogenesis was evaluated by Immunofluorescence staining and western blot. Decellularized lymph nodes showed virtually complete absence of cellular material and maintenance of lymph node architecture. The hADSCs were significantly observed in recellularized lymph node-scaffolds group. The recellularized lymph node-scaffold group was histologically similar to normal lymph nodes. The vascular endothelial growth factor A and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) in immunofluorescence staining were highly expressed in recellularized lymph node-scaffolds group. Also, the expression of LYVE-1 protein significantly increased in recellularized lymph node-scaffold group compared with others. Recellularized lymph node -scaffold had a much better therapeutic effect than stem cells or decellularized lymph node-scaffold alone, and induced stable lymphangiogenesis.

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Section: Discussionmentioning

confidence: 96%

Recellularized lymph node scaffolds with human adipose-derived stem cells enhance lymph node regeneration to improve lymphedema

Kang

Moon

Kim

et al. 2023

Sci Rep

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“…Foot and ankle volume are subjected to changes secondary to aerobic activities, lower-extremity trauma, or pathologic conditions [2,4]. The change following a surgical treatment is also important to consider [34], especially since the technique of the water basin, because of the wounds, is not suitable. The use of 3D optical scanners to track foot and ankle volume following surgery would also be an improvement.…”

Section: Discussionmentioning

confidence: 99%

“…In both clinical and research contexts, it is critical to have a reliable and simple method for determining foot volume when it comes to evaluate peripheral edema or certain types of foot and ankle injuries [34] . While "pitting" in clinical settings and the use of a water Thus, our primary objective was to assess the reliability of a 3D portable scanner for obtaining volumetric measurements of the foot and ankle, in comparison with water volumetry, considered as the reference method.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Foot and Ankle Edema With a 3D Portable Scanner

Beldame¹,

Sacco²,

Munoz³

et al. 2023

Preprint

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Background. To prospectively evaluate the reliability of a portable optical scanner compared to the water displacement technique for volumetric measurements of the foot and ankle, and to compare the acquisition time associated with these two methods. Methods. Foot volume was measured in 29 healthy volunteers (58 feet, 24 female/5 male) by a 3D scanner (UPOD-S 3D Laser Full-Foot Scanner ®) and by water-displacement volumetry. Measurements were performed on both feet, up to a height of 10 cm above the ground. The acquisition time for each method was evaluated. Kolmogorov-Smirnov test, Lin's Concordance Correlation Coefficient, and a Student’s t-test were performed. Results: Mean foot volume was 869.7+/-165.1cm3 (3D scanner) versus 867.9+/-155.4cm3 (water-displacement volumetry) (p <10-5). Concordance of measurements was 0.93, indicative of a high correlation between the two techniques. Volumes were 47.8 cm3 lower when using the 3D scanner versus water volumetry. After statistically correcting this underestimation, the concordance was improved (0.98, residual bias = -0.03 +/- 35.1 cm3). Mean examination time was 4.2 +/-1.7 min (3D optical scanner) versus 11.1 +/-2.9 min (water volumeter) (p<10-4). Conclusion: Ankle/foot volumetric measurements performed using this portable 3D scanner are reliable and fast, and can be used in clinical practice and research.

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“…Overall, 24 female and 5 male subjects were included (mean age 35.6 +/− 9.5 years, range 9-55). Self-declared shoe size was 38.17 +/− 3.23 (range [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45]. Demographic characteristics are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…In both clinical and research contexts, it is critical to have a reliable and simple method for determining foot volume when it comes to evaluating peripheral edema or certain types of foot and ankle injuries [34]. While "pitting" in clinical settings and the use of water volumetry or tape measurements (either ankle circumference or figure of eight) in research settings are considered the gold standard procedures, these techniques are prone to human error in measurements.…”

mentioning

confidence: 99%

Assessment of the Efficiency of Measuring Foot and Ankle Edema with a 3D Portable Scanner

Beldame¹,

Sacco

Munoz

et al. 2023

Bioengineering

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Background: To prospectively evaluate the reliability of a portable optical scanner compared to the water displacement technique for volumetric measurements of the foot and ankle and to compare the acquisition time associated with these two methods. Methods: Foot volume was measured in 29 healthy volunteers (58 feet, 24 females and 5 males) by a 3D scanner (UPOD-S 3D Laser Full-Foot Scanner®) and by water displacement volumetry. Measurements were performed on both feet, up to a height of 10 cm above the ground. The acquisition time for each method was evaluated. The Kolmogorov-Smirnov test, Lin’s Concordance Correlation Coefficient, and a Student’s t-test were performed. Results: Mean foot volume was 869.7 +/− 165.1 cm3 (3D scanner) versus 867.9 +/− 155.4 cm3 (water-displacement volumetry) (p < 10−5). The concordance of measurements was 0.93, indicative of a high correlation between the two techniques. Volumes were 47.8 cm3 lower when using the 3D scanner versus water volumetry. After statistically correcting this underestimation, the concordance was improved (0.98, residual bias = −0.03 +/− 35.1 cm3). The mean examination time was 4.2 +/− 1.7 min (3D optical scanner) versus 11.1 +/− 2.9 min (water volumeter) (p < 10−4). Conclusions: Ankle/foot volumetric measurements performed using this portable 3D scanner are reliable and fast and can be used in clinical practice and research.

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Lymphatic Tissue Bioengineering for the Treatment of Postsurgical Lymphedema

Cited by 8 publications

References 137 publications

Recellularized lymph node scaffolds with human adipose-derived stem cells enhance lymph node regeneration to improve lymphedema

Recellularized lymph node scaffolds with human adipose-derived stem cells enhance lymph node regeneration to improve lymphedema

Assessment of Foot and Ankle Edema With a 3D Portable Scanner

Assessment of the Efficiency of Measuring Foot and Ankle Edema with a 3D Portable Scanner

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