Lymphocyte membrane alterations caused by nylon wool column separation

Corrigan, Antonia; O’Kennedy, Richard; Smyth, Honor

doi:10.1016/0022-1759(79)90298-9

Cited by 17 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T cell enrichment by this method relies on preferential B cell and some APC adherence to nylon wool and provides only modest T cell purification (2-fold or less in our hands). Within a few years of the introduction of this purification method, several reports suggested adverse effects on T cell functions when using nylon wool (Corrigan et al, 1979; Tardieu et al, 1975). Chief among these effects was reduced T cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, expression of CD69, an early T cell activation marker, was reduced and CD25 expression was markedly increased on both CD4 + and CD8 + T cells purified by nylon wool. It is interesting to note that nylon wool treatment strikingly alters lymphocyte membranes depleting phospholipids, cholesterol and sialic acid (Corrigan et al, 1979). These results raise the possibility that the unusual T cell activation profile we observed on nylon wool treatment may be due to changes in the membrane composition of lipid rafts where T cell activation components are concentrated (Manes and Viola, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Despite its widespread use, there are reports suggesting alterations in T cell function after nylon wool enrichment. These alterations include changes in proliferative capacity (Gunzer et al, 2001; Hrabak et al, 1985), and changes in lymphocyte membrane composition (Corrigan et al, 1979). In the course of studies examining the role of complement receptors on T cells, we found that nylon wool purification of T cells altered T cell responses in a variety of assays.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nylon wool purification alters the activation of T cells

Wohler

Barnum

2009

Molecular Immunology

View full text Add to dashboard Cite

Purification of lymphocytes, particularly T cells, is commonly performed using nylon wool. This enrichment method selectively retains B cells and some myeloid cells allowing a significantly more pure T cell population to flow through a nylon wool column. T cells purified in this fashion are assumed to be unaltered and functionally naïve, however some studies have suggested aberrant in vitro T cell responses after nylon wool treatment. We found that nylon wool purification significantly altered T cell proliferation, expression of activation markers and production of cytokines. Our results suggest that nylon wool treatment modifies T cell activation responses and that caution should be used when choosing this purification method.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nylon wool purification alters the activation of T cells

Wohler

Barnum

2009

Molecular Immunology

View full text Add to dashboard Cite

show abstract

“…2 Ci/mM; Amershatn International, Amersham, U.K.) was added and eulture was continued for a further 18 h. Incorporated -'H-thytnidine was quantitated by liquid scintillation spectroscopy. Whole spleen leukocytes rather than purified T cells were used as responders in these studies as commonly used methods for purifying T cells sueh as nylon wool or erythrocyte roselting eause gross alterations in membrane biochemistry and structure (16,17) and unfractionated leukocytes have previously been used to study allogeneie cell stimulation by LC (18,19). Cultures were carried out in replicates of six and the means calculated.…”

Section: Mixed Epidermal Eell-lymphoeyte Reaetion (Meclr)mentioning

confidence: 99%

All‐trans retinoic acid induces functional maturation of epidermal Langerhans cells and protects their accessory function from ultraviolet radiation

Dunlop

Halliday

Barnetson

1994

Experimental Dermatology

View full text Add to dashboard Cite

Retinoids provide some protection against ultraviolet radiation-induced skin damage. We have previously shown that topical all-trans retinoic acid prevents ultraviolet light from reducing the density of epidermal Langerhans cells in the epidermis but does not inhibit the development of immunosuppression to a locally applied contact sensitizer. We therefore investigated the ability of all-trans retinoic acid to modulate Langerhans cell induction of allogeneic T-cell proliferation in the mixed epidermal cell lymphocyte reaction. Langerhans cells isolated from all-trans retinoic acid-treated mice induced an enhanced mixed epidermal cell lymphocyte reaction. This is similar to Langerhans cells cultured with granulocyte-macrophage colony stimulating factor. Retinoic acid treatment also enhanced the allogeneic cell-stimulating capability of Langerhans cells isolated from ultraviolet-irradiated mice. Langerhans cells from all-trans retinoic acid-treated, ultraviolet-irradiated mice which were "matured" by 3 days in culture induced a larger mixed epidermal cell lymphocyte reaction than mice treated with solvent and ultraviolet irradiation. Thus all-trans retinoic acid treatment of mice causes Langerhans cell maturation and inhibits ultraviolet light from reducing their density or impairing their allogeneic cell-stimulating capacity. However, these mice remained immunosuppressed upon application of a contact sensitizer to irradiated or unirradiated skin. It is thus likely that, whereas all-trans retinoic acid protects local Langerhans cell numbers and function, it does not inhibit the production of an ultraviolet radiation-induced photoproduct which causes immunosuppression.

show abstract

Separation of B‐ and T‐lymphocytes by cellular adsorption chromatography using poly(2‐hydroxyethyl methacrylate)/polyamine graft copolymer as column adsorbent

Maruyama

Tsuruta

Kataoka

et al. 1988

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

A series of poly(2-hydroxyethyl methacrylate)/polyamine graft copolymers (HA copolymers) were examined as an adsorbent for cellular adsorption chromatography in order to separate cell populations with high efficacy. The capability of each copolymer sample to selectively adsorb rat lymphocyte subpopulations (B-cells and T-cells) was evaluated by applying a lymphocyte suspension to a copolymer-coated glass bead column. A separation factor (AB/AT) greater than 5.0 was achieved for the HA copolymer, compared to 1.9 and 1.1 for homopolymers of poly(2-hydroxyethyl methacrylate) and of a polyamine (poly(N,N-diethylaminoethylstyrene], respectively. By loading a rat lymphocyte suspension containing B-cells (28%) and T-cells (72%) through the HA copolymer column, more than 60% of T-cells in 90% purity or more were recovered in the effluent.

show abstract

Lymphocyte membrane alterations caused by nylon wool column separation

Cited by 17 publications

References 13 publications

Nylon wool purification alters the activation of T cells

Nylon wool purification alters the activation of T cells

All‐trans retinoic acid induces functional maturation of epidermal Langerhans cells and protects their accessory function from ultraviolet radiation

Separation of B‐ and T‐lymphocytes by cellular adsorption chromatography using poly(2‐hydroxyethyl methacrylate)/polyamine graft copolymer as column adsorbent

Contact Info

Product

Resources

About