Lysinimonas soli gen. nov., sp. nov., isolated from soil, and reclassification of 
               
                  Leifsonia kribbensis
               
             Dastager et al. 2009 as Lysinimonas kribbensis sp. nov., comb. nov.

International Journal of Systematic and Evolutionary Microbiology

Jang

et al. 2013

Self Cite

Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air A Gram-positive, aerobic, rod-shaped bacterial strain, 5116S-4 T , was isolated from an air sample collected in Suwon city, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain was a new member of the family Microbacteriaceae. The sequence similarities of this strain to the members of the family Microbacteriaceae were less than 97 %, with the highest values observed with Cryobacterium mesophilum MSL-15 T (96.3 %), Cryobacterium roopkundense RuGl7 T (96.2 %), Labedella gwakjiensis KSW2-17 T (96.2 %), Cryobacterium luteum Hh15 T (96.2 %), Cryobacterium psychrophilum DSM 4854 T (96.2 %), Klugiella xanthotipulae 44C3 T (96.0 %) and Amnibacterium kyonggiense KSL51201-037 T (96.0 %). According to the phylogenetic tree, strain 5116S-4 T formed a cluster with A. kyonggiense KSL51201-037 T , Labedella gwakjiensis KSW2-17 T and Lysinimonas soli SGM3-12 T (95.3 % sequence similarity) on the support of high bootstrap values. Cells were motile with single polar flagellum and showed optimum growth at 30 6C and pH 7 without NaCl. Predominant cellular fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . Polar lipids were diphosphatidylglycerol, dimannosyldiacylglycerol, phosphatidylglycerol, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The acyl type of the cell-wall muramic acid was the acetyl type. Peptidoglycan was supposed to be the type B1 with 2,4-diaminobutyric acid on position 3. Strain 5116S-4 T was clearly distinguishable from the phylogenetically related genera in the family Microbacteriaceae in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, Naasia aerilata gen. nov., sp. nov. The type strain of Naasia aerilata is 5116S-4 T (5KACC 15517 T 5NBRC 108725 T ).

mentioning

confidence: 59%

Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air

Weon

International Journal of Systematic and Evolutionary Microbiology

Jang

et al. 2013

Self Cite

“…bacterio.net/classifgenerafamilies.html). Recently, the new genera Alpinimonas, Diaminobutyricimonas, Lysinimonas, Naasia and Pontimonas, isolated from air samples, seawater and silt, have been reported as new members of the family Microbacteriaceae (Jang et al, 2012(Jang et al, , 2013aSchumann et al, 2012;Weon et al, 2013). We isolated two strains, KIS82-1 T and GRS42 T , from soil and from sap of the tree Acer mono, respectively.…”

mentioning

confidence: 89%

“…Table 1. Differential characteristics between strain KIS82-1 T and members of related genera within the family Microbacteriaceae Taxa: 1, KIS82-1 T ; 2, Alpinimonas (data from Schumann et al, 2012);3, Frigoribacterium (Kämpfer et al, 2000;Dastager et al, 2008); 4, Frondihabitans (Zhang et al, 2007;Greene et al, 2009;Lee, 2010;Cardinale et al, 2011); 5, Labedella (Lee, 2007); 6, Lysinimonas (Dastager et al, 2009;Jang et al, 2013a). Description of Galbitalea gen. nov.…”

mentioning

confidence: 99%

Description of Galbitalea soli gen. nov., sp. nov., and Frondihabitans sucicola sp. nov.

International Journal of Systematic and Evolutionary Microbiology

Lim

Ahn

et al. 2014

Self Cite

Bacterial strains KIS82-1T and GRS42T were isolated from soil and from sap of Acer mono, respectively, in the Republic of Korea. Both strains were aerobic, Gram-stain-positive, mesophilic, rod-shaped and motile. Phylogenetically, both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria . The 16S rRNA gene sequence of strain KIS82-1T showed the highest similarity to those of Frondihabitans peucedani RS-15T (97.6 %), Frigoribacterium mesophilum MSL-08T (97.2 %) and Labedella gwakjiensis KSW2-17T (97.0 %), while strain GRS42T showed the highest 16S rRNA gene sequence similarity to Frondihabitans peucedani RS-15T (98.7 %), Frondihabitans cladoniiphilus CafT13T (98.4 %), Frondihabitans australicus E1HC-02T (98.2 %) and Frigoribacterium faeni 801T (97.3 %). The 16S rRNA gene sequence similarity between GRS42T and KIS82-1T was 97.0 %. Phylogenetic trees indicated that strain GRS42T was firmly grouped into the genus Frondihabitans , while strain KIS82-1T did not show a clear affiliation to any genus within the family Microbacteriaceae . Strain KIS82-1T showed type B1β peptidoglycan with 2,4-diamino-l-butyric acid as the diamino acid. It had MK-11, MK-10 and MK-12 as respiratory quinones, anteiso-C15 : 0, iso-C16 : 0 and iso-C14 : 0 as major cellular fatty acids and diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid as predominant polar lipids. The peptidoglycan of strain GRS42T was of type B2β with d-ornithine as the diamino acid. The strain contained MK-8, MK-9 and MK-7 as respiratory quinones, summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) as major cellular fatty acid and diphosphatidylglycerol, phosphatidylglycerol and three unknown glycolipids as predominant polar lipids. Strain GRS42T revealed low DNA–DNA hybridization (<50 % relatedness) with closely related strains. Based on the data obtained in the present polyphasic taxonomic study, we propose that strain KIS82-1T represents a novel genus and species and that strain GRS42T represents a novel species in the family Microbacteriaceae . The genus Galbitalea gen. nov. is proposed, with strain KIS82-1T ( = KACC 15520T = NBRC 108727T) as the type strain of the type species, Galbitalea soli sp. nov. Strain GRS42T ( = KACC 15521T = NBRC 108728T) is proposed as the type strain of Frondihabitans sucicola sp. nov.

“…Members of the family Microbacteriaceae have been found in diverse environments of plants, soil, air, dairy products, sewage, mushrooms, compost, insects and groundwater. Recently, new genera such as Homoserinimonas, Compostimonas, Lysinimonas and Naasia, which have been isolated from environments such as air, soil and cotton composts, have been reported to be members of the family Microbacteriaceae (Kim et al, 2012b, c;Jang et al, 2013) We isolated one bacterial strain, 5GHs34-4 T , from a soil sample from a greenhouse used to cultivate cucumbers in the Yongin region, Republic of Korea. The soil sample was serially diluted with 0.85 % saline and the suspension was plated on R2A agar (Difco).…”

mentioning

confidence: 99%

Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil

International Journal of Systematic and Evolutionary Microbiology

Moon

Hamada

et al. 2013

Self Cite

A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4 T , was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10-37 6C (optimum 28-30 6C) and at pH 5.0-9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1-97.0 % with species of the genus Leifsonia, 95.7-96.7 % with species of the genus Herbiconiux, 95.1-96.4 % with species of the genus Salinibacterium and 96.1 % with Labedella gwakjiensis and Homoserinimonas aerilata. The highest sequence similarities (97.0 %) were with Leifsonia aquatica JCM 1368 T , Leifsonia poae VKM Ac-1401 T and Leifsonia psychrotolerans LI1 T . The peptidoglycan type determined for strain 5GHs34-4 T was B2c with DL-2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c), anteiso-C 17 : 0 and anteiso-C 15 : 0 . The phenotypic and phylogenetic traits of strain 5GHs34-4 T differed in some respects from those of members of the family Microbacteriaceae. Therefore, strain 5GHs34-4 T is considered to represent a novel species of a new genus in the family Microbacteriaceae, for which the name Rudaibacter terrae gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4 T (5KACC 15523 T 5NBRC 108754 T ).