Lysogenic Conversion in Staphylococci

Waart, J. de; Winkler, Kurt; Grootsen, C.

doi:10.1038/195407a0

Cited by 17 publications

(8 citation statements)

References 4 publications

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“…For instance, heritable properties of staphylococci dependent on the presence of prophage have been reported by a number of workers (Blair & Carr, 1961;Wahl & Fouace, 1961;de Waart, Winkler & Grootsen, 1962). However, since the Tets and Penase-variants are immune to phage released by the parental strain, it does not seem possible that the observed alterations are associated with the loss of prophage.…”

Section: Discussionmentioning

confidence: 97%

The Effect of Growth at Elevated Temperatures on Some Heritable Properties of Staphylococcus aureus

Houghton

Perret

1964

Journal of General Microbiology

127

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SUMMARYPopulations of a predominantly tetracycline-resistant, penicillinasepositive strain of Staphylococcu8 aureus grown at 43-44' gave rise to progressively increasing proportions of tetracycline-sensitive and penicillinasenegative cocci. The losses did not appear until after the elapse of a number of generations at the elevated temperature, and then apparently proceeded independently, tetracycline resistance being lost more rapidly than the ability to produce penicillinase. Tetracycline-sensitive and penicillinasenegative variants were extremely stable and the growth rates a t 44' of the parent strain and the tetracycline-sensitive variants were indistinguishable. Screening for numerous other 'marker ' properties revealed no changes under the test conditions. The evidence suggests that tetracycline resistance and penicillinase-forming ability probably depend on the presence of two different plasmids in the cocci; that the replication-rates of the plasmids at elevated temperature are less than that of the cocci; and that the plasmids, once lost, are not spontaneously resynthesized nor, at least for that conferring tetracycline resistance, regained by infection. However, such a hypothesis raises the question of how equilibrium, particularly between the tetracycline-resistant and -sensitive cocci, is maintained in a population growing a t 37' and observed to change in one direction but never in the reverse direction.

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Section: Discussionmentioning

confidence: 97%

The Effect of Growth at Elevated Temperatures on Some Heritable Properties of Staphylococcus aureus

Houghton

Perret

1964

Journal of General Microbiology

127

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“…Several hundreds of survivor colonies were scored for p-toxin on sheep-blood agar plates, and for lysogenicity by replicating on indicator plates or by cross-streaking (de Waart et al 1962, Toxin production. Toxic filtrates were produced by inoculating 1 ml.…”

Section: Methodsmentioning

confidence: 99%

“…Lysogenization of certain strains of Staphylococcus aureus which produced Bhaemolysin with phages belonging to the serological group F resulted in the loss of the ability to form p-toxin (De Waart, Winkler & Grootsen, 1962; De Waart, 1964). This proved to be a true lysogenic conversion (phage conversion) since each lysogenized coccus lost the character and each colony which did not produce P-toxin proved to be lysogenic.…”

Section: Introductionmentioning

confidence: 99%

Lysogenic Conversion of Staphylococci to Loss of -Toxin

Winkler¹,

Waart²,

Grootsen³

et al. 1965

Journal of General Microbiology

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“…The capacity of aureophages to cause lysogenic conversion of several enzymes thought to be associated with staphylococcal virulence has been demonstrated (de Waart et al, 1962;Rosendal et al, 1964;Jollick, 1972;Kondo et al,, 1973;Mason and Allen, 1975;Kondo and Fujise, 1977;DuvalIflah et al, 1977;Betley and Mekalanos, 1985;Lee and Iandolo, 1985). It is also well established that these phages act as vectors for antibiotic-resistance genes and mediate the conjugative transfer of plasmid and chromosomal genes (Lacey, 1980;Schaefler, 1982).…”

Section: Introductionmentioning

confidence: 99%