Lysophosphatidic acid and human erythrocyte aggregation

Sheremet’ev, Yu. A.; Поповичева, А. Н.; Levin, G. Ya.

doi:10.1134/s1990519x14030110

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Even if the implications of echinocytosis and externalization of PS are not clear yet, these events has been described as a signal for RBC apoptosis [ 64 ]. However, there are also reasons to believe that the presence of echinocytes can support blood clotting, since their formation leads to the development and release of microvesicles [ 67 , 68 ]. These RBC-derived microvesicles have been said to have a procoagulant activity and to promote thrombin generation [ [69] , [70] , [71] ], while hindering anticoagulation processes [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

Green hemostatic sponge-like scaffold composed of soy protein and chitin for the treatment of epistaxis

Jimenez-Martin

Heras

Etxabide

et al. 2022

Materials Today Bio

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Section: Resultsmentioning

confidence: 99%

Green hemostatic sponge-like scaffold composed of soy protein and chitin for the treatment of epistaxis

Jimenez-Martin

Heras

Etxabide

et al. 2022

Materials Today Bio

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“…An objective with a magnification of 100 x was immersed into the mixture. The total magnification of the microscope was 1000x [21].…”

Section: 5aggregate Morphologymentioning

confidence: 99%

Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

2021

Preprint

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We study the influence of trypsin on aggregation, disaggregation, and aggregate morphology of RBCs in autologous plasma and serum. The effect of trypsin on the surface charge of red blood cells and the aggregation of glutaraldehyde fixed cells after treatment with the enzyme was also studied. RBC aggregation was studied by means of an aggregometer and microscopic observations. The results obtained in this study indicate that trypsin treatment increases RBCs aggregation in autologous plasma and serum. The disaggregation of erythrocytes after trypsin treatment considerably decreased in autologous plasma and serum. Increase in the strength of red blood cell aggregates was observed in autologous plasma and serum. The microscopic images of RBCs aggregates indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. Trypsin decrease the surface charge of RBCs. In autologous plasma and serum, the cup shapes of RBCs appear. The control RBCs fixed with glutaraldehyde were not aggregated after their placement in autologous plasma. At the same time, red blood cells pretreated with trypsin and fixed with glutaraldehyde interact with each other in autologous plasma. The physiological significance of glycoproteins of erythrocyte surface for RBCs aggregation was discussed.

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“…[6] в нашей модификации [7]. Описание 2) Морфологию агрегатов в аутологичной плазме изучали с помощью светового микроскопа Primo Star (Carl Zeiss, Германия) методом, предложенным авторами [8].…”

Section: методикаunclassified

Hemorheological disorders in children with thermal trauma

Левин¹,

Поповичева²,

Соснина³

2020

ZHurnal «Patologicheskaia Fiziologiia I Eksperimental`naia Terapiia»

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Цель исследования - изучение динамики изменения реологических свойств крови и их корреляции с параметрами эритроцитов в процессе лечения детей после термической травмы. Методика. Исследование проведено на 32 детях в возрасте от 3 до 17 лет с ожогами площадью от 10 до 70% поверхности тела в период лечения, начиная с 3-х суток после термической травмы и до полного закрытия ожоговых ран и выписки пациента из стационара. Изучали состояние цитоскелета эритроцитов методом термоиндукции, их агрегацию и дезагрегацию с помощью модифицированного нами реоскопа Н. Schmid-Schönbein и соавт. [1973], их деформируемость и морфологию агрегатов эритроцитов, а также рассчитывали эритроцитарные индексы. Результаты. Установлено, что после ожога происходит резкое усиление агрегации эритроцитов и прочности агрегатов, но значительно снижается их деформируемость. Нарушение реологических свойств крови сопровождает все стадии ожоговой болезни и сохраняется при выписке больных из стационара. Нарушения гемореологии коррелируют с изменениями концентрации фибриногена, состоянием цитоскелета эритроцитов и их физическими свойствами - размером эритроцитов и содержанием в них гемоглобина. Заключение. Стойкие и выраженные нарушения реологических свойств крови у детей при ожоговой болезни могут являться важнейшей причиной редукции снабжения кислородом жизненно важных органов и развития их недостаточности. Простым, хотя и предварительным, методом оценки состояния гемореологии может служить измерение эритроцитарных индексов. Aim. To study hemorheological changes and their correlation with the size of red blood cells (RBCs) during the treatment of thermal injury in children. Methods. The study included 32 children ages 3 to 17 with burns affecting 10% to 70% of the total body area during the treatment period between the 3rd day after the burn and complete wound closure. Aggregation and disaggregation of red blood cells was analyzed using a H. Schmid-Schönbein et al. [1973] rheoscope in our modification. In addition, erythrocyte deformability, morphology of erythrocyte aggregates, and the state of erythrocyte cytoskeleton were studied using thermoinduction, and erythrocyte indices were calculated. Results. After a burn injury, erythrocyte aggregation and the strength of erythrocyte aggregates were increased whereas their deformability was decreased. Disorders of blood rheology accompanied all stages of burn disease and remained at the patient discharge. Hemorheological disorders correlated with changes in fibrinogen concentrations and with changes in the erythrocyte cytoskeleton, size, and the amount of contained hemoglobin. Conclusion. The stable and pronounced disorders of blood rheological properties in children with burn disease may be an essential cause for reduction of oxygen supply to vital organs and their failure. The measurement of erythrocyte indices may represent a simple though preliminary method for assessing hemorheology.

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Lysophosphatidic acid and human erythrocyte aggregation

Cited by 4 publications

References 30 publications

Green hemostatic sponge-like scaffold composed of soy protein and chitin for the treatment of epistaxis

Green hemostatic sponge-like scaffold composed of soy protein and chitin for the treatment of epistaxis

Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

Hemorheological disorders in children with thermal trauma

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