Lysosomal membrane stability and metallothionein content in Mytilus galloprovincialis (L.), as biomarkers

Domouhtsidou, G.P.; Dailianis, Stefanos; Kaloyianni, Martha; Dimitriadis, V.K.

doi:10.1016/j.marpolbul.2004.01.013

Cited by 95 publications

(29 citation statements)

References 56 publications

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“…MT levels in the whelk T. clavigera from 11 sites in the coastal waters of Hong Kong were significantly correlated with Cd body concentrations and a clear relationship between the dis-solved Cd exposure and the resultant MT levels in the animals existed, indicating the credibility of MT as a biomarker for the evaluation of some related metal exposure [88]. Simultaneous study of MT contents and lysosomal membrane stability (LMS) in Mytilus galloprovincialis (L.) showed that MT contents were significantly less and LMS values were significantly greater in mussels collected from the reference station compared to those from heavy metal (Cd, Pb, Cu and Zn) polluted sites in the Gulf of Thermaikos [89], showing the feasibility of actual use of MT in the assessment of environmental pollution. Metallothionein mRNA was also reported to be implicated for biomonitoring.…”

Section: Biochemical Alterationsmentioning

confidence: 99%

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Zhou

Zhang

et al. 2008

Analytica Chimica Acta

765

348

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Section: Biochemical Alterationsmentioning

confidence: 99%

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Zhou

Zhang

et al. 2008

Analytica Chimica Acta

765

348

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“…• They can detect exposure to and the toxic effects of parent compounds and metabolites of readily metabolisable and eliminated contaminants such as PAHs and organophosphate. • They can integrate the toxicological interactions of mixtures of contaminants such as mixtures of various congeners of PCBs, PAHs, metals (Domouthsidou et al 2004) to give an expression of the cumulative effect at a particular molecular, cellular or tissue target (Siu et al 2003). • They can act as short-term predictors of long-term ecological effects through integration into a suite of measurements and understanding at different levels of contamination (Behrens and Segner, 2005).…”

Section: Significance Of the Use Of Biomarkermentioning

confidence: 99%

“…The results are generally positive, in particular when the metal gradient of pollution is quite real. More and more of studies in situ combine the quantification of several biomarkers, of which MT is only one (Carajaville et al 2000;Petrovic et al 2001;Blaise et al 2002;Gagné et al 2002;Chèvre et al 2003;Giguère et al 2003;Domouthsidou et al 2004).…”

Section: Metallothionein Inductionmentioning

confidence: 99%

“…This paper highlights the significance of such biomarkers in terms of their application and current limitations. It is mainly focused on biomarkers at an advanced stage of usage and development such as cytochrome P-4501A (Domouthsidou et al 2004;Isani et al 2000), DNA integrity (Siu et al 2003;Burmeister et al 2004;Everaarts and Sarkar 1996), DNA adducts, acetycholinesterase activity (Binelli et al 2006;Escartin and Porte 1997;Fuentes-Rios et al 2005) and metallothioneins (MTs) (Petrovic et al 2001) which are specific to impact by pollution and additionally whose application would benefit from commercial and biotechnological development.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Biomarkers: Their significance and application in marine pollution monitoring

et al. 2006

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This paper presents an overview of the significance of the use of molecular biomarkers as diagnostic and prognostic tools for marine pollution monitoring. In order to assess the impact of highly persistent pollutants such as polychlorinated biphenyls (PCB), polychlorinated dibenzo-dioxins (PCDD), polychlorinated dibenzo-furans (PCDF), polynuclear aromatic hydrocarbons (PAH), tributyltin (TBT) and other toxic metals on the marine ecosystem a suite of biomarkers are being extensively used worldwide. Among the various types of biomarkers, the following have received special attention: cytochrome P4501A induction, DNA integrity, acetylcholinesterase activity and metallothionein induction. These biomarkers are being used to evaluate exposure of various species of sentinel marine organisms (e.g. mussels, clams, oysters, snails, fishes, etc.) to and the effect of various contaminants (organic xenobiotics and metals) using different molecular approaches [biochemical assays, enzyme linked immuno-sorbent assays (ELISA), spectrophotometric, fluorometric measurement, differential pulsed polarography, liquid chromatography, atomic absorption spectrometry]. The induction of the biotransformation enzyme, cytochrome P4501A in fishes (Callionymus lyra, Limanda limanda, Serranus sp., Mullus barbatus) and mussels (Dreissena polymorpha) by various xenobiotic contaminants such as PCBs, PAHs, PCDs is used as a biomarker of exposure to such organic pollutants. The induction of cytochrome P4501A is involved in chemical carcinogenesis through catalysis of the covalent bonding of organic contaminants to a DNA strand leading to formation of DNA adduct. Measurement of the induction of cytochrome P4501A in terms of EROD (7-ethoxy resorufin O-deethylase) activity is successfully used as a potential biomarker of exposure to xenobiotic contaminants in marine pollution monitoring. In order to assess the impact of neurotoxic compounds on marine environment the evaluation of acetylcholinesterase activity in marine organisms is used as a biomarker of exposure to neurotoxic agents such as organophosphorus, carbamate pesticides etc. Metallothioneins (MTs) are induced by toxic metals such as Cd, Hg, and Cu by chelation through cysteine residues and are used in both vertebrates and invertebrates as a biomarker of metal exposure. The measurement of the levels of DNA integrity in marine organisms such as Sea stars (Asterias rubens) from the North Sea and the marine snails (Planaxis sulcatus) from the Arabian Sea along the Goa coast exposed to environmental xenobiotic contaminants clearly indicated the extent and the nature of pollution at the sampling sites along coastal environment.

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“…The digestive cell lysosomes are involved in intracellular digestion and pollutant accumulation and detoxification Domouhtsidou et al, 2004;Robledo et al, 2006;Izagirre et al, 2008). Also, lysosomes play a key role in defence against oxidative stress (Moore et al, 2006), which is known to be induced during extreme thermal exposure (Abele et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Lysosomal responses to heat-shock of seasonal temperature extremes in Cd-exposed mussels

2015

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Lysosomal membrane stability and metallothionein content in Mytilus galloprovincialis (L.), as biomarkers

Cited by 95 publications

References 56 publications

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystem

Molecular Biomarkers: Their significance and application in marine pollution monitoring

Lysosomal responses to heat-shock of seasonal temperature extremes in Cd-exposed mussels

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