Macromolecule–semiconductor interfaces: From enzyme immobilization to photoelectrocatalytical applications

Skorupska, K.; Lewerenz, H.J.; Smith, James R.; Kulesza, P.J.; Mernagh, D.; Campbell, S.A.

doi:10.1016/j.jelechem.2011.05.020

Cited by 7 publications

(3 citation statements)

References 76 publications

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“…Positively charged areas on the protein surface exist that are connected with the basic amino-acids like lysine, arginine and histidine. [40] The deposition is thought to occur via electrostatic interaction of these parts with the negatively charged sites near the step edges of the step-bunched Si. For the AMV-RT, the isoelectric point is 6.5.…”

Section: Electrodeposition Of Reverse Transcriptases Ontomentioning

confidence: 99%

Fundamental Aspects of Electrodeposition for the Realization of Plasmonic Nanostructures

Muñoz¹,

Skorupska²,

Lewerenz³

2010

ChemPhysChem

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Electrodeposition is used for the preparation of nanoparticles and nanostructures that allow, in principle, surface plasmon excitation. The (photo)electrodeposition process of Rh and Au nanoparticles as well as of heterodimeric enzymes onto silicon surfaces is investigated and the resulting structures are discussed with regard to applications in photoelectroctalysis and biosensing. Electrodeposition of Rh onto H-terminated p-Si surfaces generates nanostructures of the metal nanoparticles with simultaneous oxidation of the substrate thus forming nano-dimensioned metal-oxide-semiconductor (MOS)-type contacts. The excess minority carrier harvesting in these nanoemitter structures, where semispherical space charge layers underneath the metal exist are discussed based on spectral sensitivity and capacitance measurements The deposition of Au nanoparticles by a combined chemical-electrochemical method on Si is presented as an example for sensing actuators where the resonance frequency is changed by adsorption. Similarly, site-selective deposition of the enzyme reverse transcriptase onto nanostructured (step-bunched) silicon serves as precursor experiment for biosensing in a Kretschmann-type ATR configuration. Future applications based on plasmonically active structures are outlined.

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Section: Electrodeposition Of Reverse Transcriptases Ontomentioning

confidence: 99%

Fundamental Aspects of Electrodeposition for the Realization of Plasmonic Nanostructures

Muñoz¹,

Skorupska²,

Lewerenz³

2010

ChemPhysChem

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“…Here, the force between a tip and sample is monitored rather than the acquisition of a tunnelling current. Strictly speaking, in the field of biology, STM could allow imaging of small biomolecules, proteins and DNA due to small tunnelling distances, albeit the conduction mechanism remains poorly understood [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Characterization of drug delivery vehicles using atomic force microscopy: current status

Smith

Olusanya

Lamprou

2018

Expert Opinion on Drug Delivery

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Introduction: The field of nanomedicine, utilising nano-sized vehicles (nanoparticles and nanofibres) for targeted local drug delivery, has a promising future. This is dependent on the ability to analyse the chemical and physical properties of these drug carriers at the nanoscale and hence atomic force microscopy (AFM), a high-resolution imaging and local force-measurement technique, is ideally suited. Areas covered: Following a brief introduction to the technique, the review describes how AFM has been used in selected publications from 2015-2018 to characterise nanoparticles and nanofibers as drug delivery vehicles. These sections are ordered into areas of increasing AFM complexity: imaging/particle sizing, surface roughness/quantitative analysis of images and analysis of force curves (to extract nanoindentation and adhesion data). Expert opinion: AFM imaging/sizing is used extensively for the characterisation of nanoparticle and nanofibre drug delivery vehicles, with surface roughness and nanomechanical/adhesion data acquisition being less common. The field is progressing into combining AFM with other techniques, notably SEM, ToF-SIMS, Raman, Confocal and UV. Current limitations include a 50 nm resolution limit of nanoparticles imaged within live cells and AFM tip-induced activation of cytoskeleton proteins. Following drug release real-time with AFM-spectroscopic techniques and studying drug interactions on cell receptors appear to be on the horizon.

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“…Pierce Dialysis Cassettes (3500 MWCO with 0.1-0.5 ml capacity) with suitable syringes and needles were used (Perbio Science). Further details are described elsewhere [9].…”

mentioning

confidence: 99%

Scanning probe characterization of enzymes deposited onto step-bunched silicon nanostructures

Skorupska¹,

Lewerenz²,

Vo‐Dinh³

2009

Phys. Scr.

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Step-bunched Si surfaces with step heights of ∼ 3 nm have been prepared as substrates for immobilization of the enzyme reverse transcriptase of the avian myeloblastosis virus. Tapping mode atomic force microscopy has been used for imaging. Calibration experiments using the carrier solution without the enzyme show the preservation of the surface without organic contamination. Upon enzyme deposition, two specific shapes of the enzymes are observed to be adsorbed on the surface after rinsing with ultrapure water. Data analysis reveals that adsorption takes place at kink and reentrant sites on the surface and along step edges but not on the planar parts of the base (111) surface. The adsorption is interpreted by a combination of electrostatic and van der Waals interaction in accordance with the framework of the DLVO 4 theory.

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Macromolecule–semiconductor interfaces: From enzyme immobilization to photoelectrocatalytical applications

Cited by 7 publications

References 76 publications

Fundamental Aspects of Electrodeposition for the Realization of Plasmonic Nanostructures

Fundamental Aspects of Electrodeposition for the Realization of Plasmonic Nanostructures

Characterization of drug delivery vehicles using atomic force microscopy: current status

Scanning probe characterization of enzymes deposited onto step-bunched silicon nanostructures

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