Macrophage-targeting bioactive glass nanoparticles for the treatment of intracellular infection and subcutaneous abscess

Zhang, Shixiong; Zhao, Lulong; Chen, Zhishu; Zhang, Linya; Li, Lichen; Zhao, Mengen; Yan, Le-Ping; Liao, Liqiong; Zhang, Chao; Wu, Zhaoying

doi:10.1039/d2bm01117d

Cited by 6 publications

(6 citation statements)

References 59 publications

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“…On day 7 post-infection, Man-BGNs/Ag treatment significantly reduced bacterial burden in skin abscesses compared with BGN-treated, Man-BGN-treated and untreated mice. As expected, a reduction in the bacterial burden resulted in an increased number of M2 macrophages and, therefore, accelerated wound healing [159].…”

Section: Bioactive Glass Nanoparticlessupporting

confidence: 77%

“…Zhang et al developed mannose-modified bioactive glass nanoparticles decorated with silver nanoparticles (Man-BGNs/Ag) to treat S. aureus skin infections [159]. To produce the Man-BGNs/Ag particles, mannose was first conjugated to the BGNs and then Ag + ions were reduced with glucose to form Ag nanoparticles on Man-BGNs [160].…”

Section: Bioactive Glass Nanoparticlesmentioning

confidence: 99%

“…However, Man-BGNs/Ag inactivated most of the intracellular bacteria. Apart from the effect of Man-BGNs/Ag on the bacteria, the NPs induced ROS production by macrophages, further improving their intracellular antibacterial efficiency [159]. The potential efficacy of the Man-BGNs/Ag in vivo treatment was evaluated in a murine skin abscess infection model.…”

Section: Bioactive Glass Nanoparticlesmentioning

confidence: 99%

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New Weapons to Fight against Staphylococcus aureus Skin Infections

Cela,

Urquiza,

Gómez

et al. 2023

Antibiotics

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The treatment of Staphylococcus aureus skin and soft tissue infections faces several challenges, such as the increased incidence of antibiotic-resistant strains and the fact that the antibiotics available to treat methicillin-resistant S. aureus present low bioavailability, are not easily metabolized, and cause severe secondary effects. Moreover, besides the susceptibility pattern of the S. aureus isolates detected in vitro, during patient treatment, the antibiotics may never encounter the bacteria because S. aureus hides within biofilms or inside eukaryotic cells. In addition, vascular compromise as well as other comorbidities of the patient may impede proper arrival to the skin when the antibiotic is given parenterally. In this manuscript, we revise some of the more promising strategies to improve antibiotic sensitivity, bioavailability, and delivery, including the combination of antibiotics with bactericidal nanomaterials, chemical inhibitors, antisense oligonucleotides, and lytic enzymes, among others. In addition, alternative non-antibiotic-based experimental therapies, including the delivery of antimicrobial peptides, bioactive glass nanoparticles or nanocrystalline cellulose, phototherapies, and hyperthermia, are also reviewed.

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Section: Bioactive Glass Nanoparticlessupporting

confidence: 77%

Section: Bioactive Glass Nanoparticlesmentioning

confidence: 99%

Section: Bioactive Glass Nanoparticlesmentioning

confidence: 99%

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New Weapons to Fight against Staphylococcus aureus Skin Infections

Cela,

Urquiza,

Gómez

et al. 2023

Antibiotics

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“…It was reported that macrophages can activate innate immune function to defend intracellular bacteria mainly by oxidative stress. − Therefore, we analyzed whether PECs can improve antibacterial activity by enhancing the oxidative stress of infected macrophages. We first detected intracellular ROS levels by 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe.…”

Section: Resultsmentioning

confidence: 99%

Rifampicin-Loaded Polyelectrolyte Complex Eliminates Intracellular Bacteria through Thiol-Mediated Cellular Uptake and Oxidative Stress Enhancement

Xia,

Liao,

Gao

et al. 2024

ACS Appl. Bio Mater.

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The poor accumulation of antibiotics in the cytoplasm leads to the poor eradication of intracellular bacteria. Herein, a polyelectrolyte complex (PECs@Rif) allowing direct cytosolic delivery of rifampicin (Rif) was developed for the treatment of intracellular infections by complexation of poly(α-lipoic acid) (pLA) and oligosaccharide (COS) in water and loading Rif. Due to the thiol-mediated cellular uptake, PECs@Rif delivered 3.9 times higher Rif into the cytoplasm than that of the free Rif during 8 h of incubation. After entering cells, PECs@Rif released Rif by dissociating pLA into dihydrolipoic acid (DHLA) in the presence of intracellular thioredoxin reductase (TrxR). Notably, DHLA could reduce endogenous Fe(III) to Fe(II) and provide a catalyst for the Fenton reaction to produce a large amount of reactive oxygen species (ROS), which would assist Rif in eradicating intracellular bacteria. In vitro assay showed that PECs@Rif reduced almost 2.8 orders of magnitude of intracellular bacteria, much higher than 0.7 orders of magnitude of free Rif. The bacteremia-bearing mouse models showed that PECs@Rif reduced bacterial levels in the liver, spleen, and kidney by 2.2, 3.7, and 2.3 orders of magnitude, respectively, much higher than free Rif in corresponding tissues. The direct cytosolic delivery in a thiol-mediated manner and enhanced oxidative stress proposed a feasible strategy for treating intracellular bacteria infection.

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“…Moreover, some oxides and metals such as titanium dioxide, copper and silver have intrinsic antimicrobial activities and are particularly attractive to be used as antimicrobial agents for intracellular bacteria treatment. [104][105][106][107][108] Ruoslahti et al developed vancomycin-loaded silver nanoparticles conjugated with a cyclic 9-amino-acid peptide CARGGLKSC (CARG) that can specifically bind to S. aureus. The vancomycin-AgNPs-CARG selectively accumulates in S. aureus-infected tissues and cells, and remarkably improves the survival of S. aureus-infected mice, but not the survival rate of Pseudomonas-infected mice.…”

Section: Inorganic Solid Nanoparticlesmentioning

confidence: 99%