Macrophages in Corpora Lutea of Mice: Characterization and Effects on Steroid Secretion

Kirsch, Ted M.; Friedman, Andrew C.; Vogel, Robert L.; Flickinger, George L.

doi:10.1095/biolreprod25.3.629

Cited by 174 publications

(72 citation statements)

References 16 publications

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“…Also, in other organs, macrophages and dendritic cells have been found which are able to regulate the function of endocrine cells, for example, the folliculo-stellate cells in the pituitary [27] and DC-like cells and macrophages in the ovary [28] and the testis [29]. The endocrine regulatory actions of these cells are partly performed in a paracrine way, but also via cell-to-cell contact [30,31].…”

Section: Discussionmentioning

confidence: 99%

Effect of Prophylactic Insulin Treatment on the Number of ER-MP23+Macrophages in the Pancreas of NOD Mice. Is the Prevention of Diabetes Based on β-cell Rest?

Jansen

Rosmalen

Homo‐Delarche

et al. 1996

Journal of Autoimmunity

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Prophylactic insulin treatment has been shown to have beneficial effects in type 1 diabetes, both in humans and in various animal models of the disease. In experimental models, the protective effect of prophylactic insulin treatment was observed in two parameters: (1) progression of insulitis and (2) diabetes incidence. The mechanism of protection still remains to be investigated. We therefore analysed by immunohistochemistry the effect of prophylactic insulin treatment vs placebo treatment (from 4 to 13 weeks of age) on ER-MP23 + macrophage infiltration in and around pancreatic islets in the non-obese diabetic (NOD) mouse, a spontaneous model for type 1 diabetes. BALB/c mice were used as diabetes-free controls. Using conventional haematoxylin-eosin staining, we detected a protective effect of prophylactic insulin treatment in NOD females on the lymphocytic insulitis, significant at 13 weeks, but not at 9 weeks of age. However, when assessed by immunostaining for early infiltration of ER-MP23 + macrophages around islets, the reduction in severity of insulitis could already be detected as early as 9 weeks of age. With regard to the early accumulation of ER-MP23 + cells, we observed that their numbers per mm 2 surface area of the exocrine pancreas and per m at the circumference of the islet were higher in placebo-treated NODs (197±13.8 and 14±0.9, respectively) as compared to age-matched BALB/c mice (123.1±7.1 and 3.5±0.9, respectively). Prophylactic insulin treatment of NODs lowered the attraction of ER-MP23 + macrophages to the exocrine pancreas and to the circumference of the islets (156.3±8.5 and 7.9±1, respectively). Interestingly also, the islet size was found to be larger in placebo-treated NODs (51% was larger than 10 m 2 ) than in age-matched BALB/c mice (9% larger than 10 m 2 ). Prophylactic insulin treatment of NODs reduced their islet size to sizes found in the control BALB/c strain. In conclusion, the decrease in islet size by early insulin administration, and the lower attraction of ER-MP23 + macrophages to the islets are morphological indications that prevention of diabetes development by prophylactic insulin treatment results from a downregulation of islet metabolism and growth, with a concomitant decline in the release of islet factors attracting macrophages.

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Section: Discussionmentioning

confidence: 99%

Effect of Prophylactic Insulin Treatment on the Number of ER-MP23+Macrophages in the Pancreas of NOD Mice. Is the Prevention of Diabetes Based on β-cell Rest?

Jansen

Rosmalen

Homo‐Delarche

et al. 1996

Journal of Autoimmunity

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“…Macrophages enhance progesterone production in cultured granulosa cells in rats [18], mice [19] and humans [20]. Our previous study showed that macrophages also promote granulosa cell proliferation in vitro [6].…”

Section: Discussionmentioning

confidence: 95%

Immunohistochemical Localization of Epidermal Growth Factor and Its Effect on Granulosa Cell Proliferation in Rat Ovary.

1995

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Abstract.The localization of epidermal growth factor (EGF) in the ovary and its effect on proliferation of granulosa cells were investigated in gonadotrophin-primed immature female rats. Immunoreactions with anti-rat EGF monoclonal antibody were observed sparsely in the granulosa layer and antrum of follicles, but not in the theca layer or stromal tissue. The EGF-positive cells were round or oval shaped and often larger than granulosa cells. The localization and morphological appearances of these cells in the follicles were in good agreement with those of macrophages.Although EGF alone did not promote granulosa cell growth in vitro, the labelling index with [3Hlthymidine of granulosa cells cultured with 0.1 ng/ml EGF and 0.1 ng/ml basic fibroblast growth factor was significantly greater than that without the growth factors (18.4% vs. 15.8%, P<0.01). These results suggest that macrophages in follicles may modulate follicular development through a paracrine mechanism by secreting EGF and other growth factors.

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“…Previous studies have shown that the mature ovary contains many macrophages which may be involved in important endocrine or paracrine processes within the organ (KIRSCH et al, 1981;HALME et al, 1985;BAGAVANDOSS et al, 1988;ADASHI, 1990;MORI, 1990;FUKUMATSU et al, 1992;STERN and COULAM, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, the physical contact of macrophages with such steroid-producing cells as luteal and theca cells attracted much attention for the consideration of the paracrine functions of ovarian macrophages (KIRSCH et al, 1981;HALME et al, 1985;BAGA-VANDOSS et al, 1988;ADASHI, 1990;MORI, 1990;FUKUMATSU et al, 1992;STERN and COULAM, 1992). We have clarified the presence of macrophagemacrophage contact through the long cell processes of great numbers pf F4/80-positive cells in the interstitium outside the corpora lutea and follicles.…”

Section: Discussionmentioning

confidence: 99%

Distribution of F4/80-Positive Cells in Developing Ovaries in the Mouse.

Itoh

Yano

et al. 1998

Archives of Histology and Cytology

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Macrophages in Corpora Lutea of Mice: Characterization and Effects on Steroid Secretion

Abstract: Cells with the ultrastructural

Cited by 174 publications

References 16 publications

Effect of Prophylactic Insulin Treatment on the Number of ER-MP23+Macrophages in the Pancreas of NOD Mice. Is the Prevention of Diabetes Based on β-cell Rest?

Effect of Prophylactic Insulin Treatment on the Number of ER-MP23+Macrophages in the Pancreas of NOD Mice. Is the Prevention of Diabetes Based on β-cell Rest?

Immunohistochemical Localization of Epidermal Growth Factor and Its Effect on Granulosa Cell Proliferation in Rat Ovary.

Distribution of F4/80-Positive Cells in Developing Ovaries in the Mouse.

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