Magnesia–zirconia based mimetic biomembrane chromatography for predicting human drug absorption

Hu, Zhongqiang; Zhang, Weinong; He, Haibo; Feng, Yu‐Qi; Da, Shi‐Lu

doi:10.1016/j.jchromb.2005.08.015

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…The development of stationary phases that provide desired separation selectivity and high separation performance is one of the most important tasks to enhance the usefulness and applicability of chromatographic methodology. There are two major approaches for exploitation of novel chromatographic stationary phases; that is, chemical modifications of known materials − and developments of new base materials. − The surface modifications of silica gel and polymer beads have been extensively studied in various branches of chemistry. The introduction of ligand molecules onto the base packing materials through an appropriate covalent bond is typically used for the preparation of these stationary phases. − In addition, the physisorption of functional molecules, such as surfactants, onto a base stationary phase is also a versatile and convenient means. − One recent innovation in base materials is the development of monolithic stationary phases. − Such stationary phases have been widely used because of their potentially high separation performance and short analysis times, similar to an open tubular capillary.…”

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confidence: 99%

Ice Chromatography. Characterization of Water−Ice as a Chromatographic Stationary Phase

Tasaki

Okada

2006

Anal. Chem.

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Water-ice has been characterized as a stationary phase for liquid chromatography. Solutes having two or more polar groups are retained on this stationary phase with THF/hexane as the mobile phase, suggesting that multipoint interactions are required for measurable solute retention. Chromatographic separation of phenols or crown ethers on water-ice is possible. The ice surface is expected to provide two different adsorption sites coming from the OH and O dangling bonds. Although the solute partition into the quasiliquid layer is also considered, the dependence of the retention times on the THF concentration implies that the interaction of solutes with the water-ice surface rather than the partition into the quasiliquid layer is responsible for solute retention. A retention model suggests that the number of adsorption sites for a crown ether depends on its ring size, whereas two sites are involved for the retention of phenols having two hydroxyl groups. Although hydroxyl groups can act as both a hydrogen bond donor and an acceptor, the interaction with the ice OH sites, which are exposed to the surroundings in comparison with the ice O sites, is more important. However, when an acyclic polyether is added to the mobile phase, its adsorption onto the water ice surface allows the creation of the O sites that phenols can approach without steric hindrance. In the presence of the polyethers adsorbed on the ice surface, the retention of phenols is enhanced, whereas crown ethers become less retained due to the competitive adsorption of the polyethers.

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confidence: 99%

Ice Chromatography. Characterization of Water−Ice as a Chromatographic Stationary Phase

Tasaki

Okada

2006

Anal. Chem.

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“…The retention of 22 bioactive compounds was determined and correlated with their absorption. It was established that the new method is suitable for the prediction of oral drug absorption (Hu et al, 2005).…”

Section: Immobilized Liposome Chromatographymentioning

confidence: 99%

Liposomes in chromatography

Cserháti

Szőgyi

2010

Biomedical Chromatography

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The newest achievements in the application of liposomes in different chromatographic technologies such as high-performance liquid chromatography and electrically driven separation methods was compiled and critically evaluated. The employment of chromatographic methodologies for the determination of molecular parameters of biological importance is also discussed in detail. The future trends of the use of liposomes is also shortly discussed.

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“…There are a number of screening methods adaptable for predicting membrane permeability, such as the parallel artificial membrane permeability assay (PAMPA) [34,35], the chromatographic methods [36][37][38][39] and the Caco-2 monolayer assay [40,41]. As the chromatographic method, immobilized artificial membrane (IAM), immobilized liposome and biopartitioning micellar chromatography (BMC) have been developed [38,39].…”

Section: Membrane Permeabilitymentioning

confidence: 99%

“…As the chromatographic method, immobilized artificial membrane (IAM), immobilized liposome and biopartitioning micellar chromatography (BMC) have been developed [38,39]. The Caco-2 monolayer assay has been widely utilized in optimizing lead structures and selecting NCEs [40,41], and several approaches for Caco-2 incubation and/or quantitative analysis of transported compounds by LC/MS/MS have been developed to increase the throughput [15,[21][22][23][24][25][26].…”

Section: Membrane Permeabilitymentioning

confidence: 99%

Pitfalls in High Throughput Screening for Drug Absorption Optimization in Drug Discovery

Kono¹,

Iwaki

2007

CAC

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Implementation of combinatorial chemistry and high throughput screening to biological targets has led to produce lipophilic and poorly water soluble lead compounds. Since these compounds show poor absorbability, high throughput methods for improving solubility and membrane permeability have been developed. Kinetic solubility of compounds after dilution of dimethyl sulfoxide solution into aqueous buffer is determined using high throughput methods with turbidimetry, nephelometry or UV assays. The kinetic solubility tends to be overestimated compared with the corresponding thermodynamic solubility obtained using a traditional flask shaking method. A new solid-dissolution method providing thermodynamic solubility similar to that in traditional method has been developed using a 96-well plate for equilibrium dialysis. In conventional Caco-2 assays, the membrane permeability (P app ) of certain lipophilic compounds was underestimated due to insolubility in the apical compartment and adhesion to the device, resulting in a poor relationship between the in vivo absorption fraction and the P app values. The addition of a solubilizer into the apical compartment and bovine serum albumin in the basolateral compartment improved the relationship, enabling evaluation of widely diverse compounds. The usefulness of these newly-developed methods for compounds with poor physical properties will be discussed, with reviewing currently available high throughput methods for optimizing absorption.

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Magnesia–zirconia based mimetic biomembrane chromatography for predicting human drug absorption

Cited by 10 publications

References 51 publications

Ice Chromatography. Characterization of Water−Ice as a Chromatographic Stationary Phase

Ice Chromatography. Characterization of Water−Ice as a Chromatographic Stationary Phase

Liposomes in chromatography

Pitfalls in High Throughput Screening for Drug Absorption Optimization in Drug Discovery

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