Magnetic Characterization and Moderate Cytotoxicity of Magnetic Mesoporous Silica Nanocomposite for Drug Delivery of Naproxen

Abstract

In this study, we describe the magnetic and structural properties and cytotoxicity of drug delivery composite (DDC) consisting of hexagonally ordered mesoporous silica, iron oxide magnetic nanoparticles (Fe2O3), and the drug naproxen (Napro). The nonsteroidal anti-inflammatory drug (NSAID) naproxen was adsorbed into the pores of MCM-41 silica after the ultra-small superparamagnetic iron oxide nanoparticles (USPIONs) encapsulation. Our results confirm the suppression of the Brownian relaxation process caused by a “gripping effect” since the rotation of the whole particle encapsulated in the porous system of mesoporous silica was disabled. This behavior was observed for the first time, to the best of our knowledge. Therefore, the dominant relaxation mechanism in powder and liquid form is the Néel process when the rotation of the nanoparticle’s magnetic moment is responsible for the relaxation. The in vitro cytotoxicity tests were performed using human glioma U87 MG cells, and the moderate manifestation of cell death, although at high concentrations of studied systems, was observed with fluorescent labeling by AnnexinV/FITC. All our results indicate that the as-prepared MCM-41/Napro/Fe2O3 composite has a potential application as a drug nanocarrier for magnetic-targeted drug delivery.

The effect of temperature on the synthesis of magnetite nanoparticles by the coprecipitation method

“…The investigation into the conductive capabilities of magnetic particles has been the focus of research due to its applicability not only in electronic devices but also in the field of medicine and diagnosis [ 5 , 12 ]. Magnetic particles endowed with conductivity have the potential to enhance the detection of low-intensity magnetic fields, providing more accurate values for diagnostic mapping in biomedicine [ 23 ].…”

“…Several examples can be cited, and they are frequently seen in the literature, such as cancer treatment by hyperthermia [ 5 ], tissue engineering and medical implants [ 6 ], and nanomaterial substrates that support stem cell growth [ 7 ]. Another technological advantage that we can gain is by combining nanoparticles with other materials, such as a surfactant [ 3 , 4 , [8] , [9] , [10] , [11] ], which brings more applications, such as drug delivery [ 12 ], selectivity to NP [ 8 , 13 ], substitution for organic contrasts [ [14] , [15] , [16] ], and increases the biocompatibility and stability of the material [ [8] , [9] , [10] , [11] ].…”

In vivo study of light-driven naproxen release from gated mesoporous silica drug delivery system

“…In our recent works 22 , 23 , 28 , 29 we have studied in vitro biocompatibility and cytotoxicity of various mesoporous silica materials, non-modified or surface ligand modified. We did cytotoxicity experiments on the U87 MG and SKBR3 cancer cells using the microscopic techniques, flow-cytometry, MTT assay, apoptosis assay and CAM assay.…”

“…Based on this knowledge and great drug delivery potential of MSNs, in our previous works, we have described the different mesoporous silica particles for pH driven drug release 22 , 23 , redox potential driven release 24 , temperature driven drug release 25 or light driven drug release 26 , 27 . In addition to physicochemical-characterisation, we studied biocompatibility and cytotoxicity of the prepared DDS in vitro on the U87 MG and SKBR3 cells using the microscopic techniques, flow-cytometry, MTT assay, apoptosis assay and CAM assay 22 , 23 , 28 , 29 . As a continuation of these works in the present study, we studied the functionality of such systems in vivo.…”

Magnetic Characterization by Scanning Microscopy of Functionalized Iron Oxide Nanoparticles

“…The study of functionalized magnetic nanoparticles (MNPs) has shown tremendous technological advantageous features for biomedical applications [ 6 , 7 , 8 ], such as MRI contrast [ 9 , 10 ] and drug delivery systems [ 11 ], to increase efficiency by releasing a specific drug in a target tissue, without possible damage to healthy tissues [ 5 , 12 , 13 ]. In the presence of a magnetic field, MNPs can be driven to a specific location, increasing the drug concentration [ 14 , 15 ].…”