Magnetic electrochemiluminescent immunoassay with quantum dots label for highly efficient detection of the tumor marker α-fetoprotein

Huang, Zhengjun; Han, Wendi; Wu, Yanhong; Hu, Xiang-Guang; Yuan, Ya-Ni; Chen, Wei; Peng, Hua-Ping; Liu, Ailin; Lin, Xinhua

doi:10.1016/j.jelechem.2016.11.060

Cited by 22 publications

(4 citation statements)

References 31 publications

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“…Therefore, they have been widely used in clinical diagnosis, clinical analysis, and cancer detection [ 237 , 238 , 239 ]. For instance, ZnSe/CdS/ZnS core–shell quantum dots have been used in the detection of C-reactive protein, an early indicator of infection and autoimmune disorders [ 237 ]; bovine serum albumin-doped CdS quantum dots have been used in detecting human IgG1, a low-abundance protein [ 240 ]; Cu:ZnInS/ZnS quantum dots have been used in tetanus antibody detection [ 241 ]; CdTe quantum dots are used for detecting α-fetoprotein, a tumor marker [ 242 ]; and S- and N–Co-doped carbon quantum dots have been used in detecting clenbuterol, a feed additive for livestock and poultry [ 239 ].…”

Section: Application Of Alloy Nanoparticles In the Biological Fielmentioning

confidence: 99%

Synthesis, Properties, and Biological Applications of Metallic Alloy Nanoparticles

Huynh

Pham

Kim

et al. 2020

IJMS

147

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Metallic alloy nanoparticles are synthesized by combining two or more different metals. Bimetallic or trimetallic nanoparticles are considered more effective than monometallic nanoparticles because of their synergistic characteristics. In this review, we outline the structure, synthesis method, properties, and biological applications of metallic alloy nanoparticles based on their plasmonic, catalytic, and magnetic characteristics.

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Section: Application Of Alloy Nanoparticles In the Biological Fielmentioning

confidence: 99%

Synthesis, Properties, and Biological Applications of Metallic Alloy Nanoparticles

Huynh

Pham

Kim

et al. 2020

IJMS

147

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“…A similar incubation time has been reported for alphafeto protein immunosensor. 5 A pH of 7 was used as the optimum pH. [46][47][48] Fig.…”

Section: Optimisation Of Temperature and Incubation Timementioning

confidence: 99%

“…Different analytical techniques such as radioimmunoassay, 2 enzyme-linked immunosorbent assay, 3 fluorescence 4 and chemiluminescence 5 have been reported for the quantification of AFP. Unfortunately, some of the problems that limit their applications include high turnaround time; high cost, instruments that are not easily operated and requires expertise.…”

Section: Introductionmentioning

confidence: 99%

Towards cancer diagnostics – an α-feto protein electrochemical immunosensor on a manganese(iv) oxide/gold nanocomposite immobilisation layer

2018

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“…Several effective technologies have been reported to enrich and separate targets from complex samples, including chromatography, magnetic nanobeads (MBs), and capillary electrophoresis. − Among them, MBs have been proven to be a powerful separation tool owing to its large specific surface area, rich functional groups, superparamagnetism, and reaction flexibility. Currently, MBs have been widely used in various analytical methods, including electrochemistry, , fluorescence, − lateral flow immunoassay, microfluidic chip, and ECL , sensors. Therefore, it is interesting to combine the idea of MBs and multiple amplification strategies to construct ECL biosensors with high sensitivity and accuracy.…”

mentioning

confidence: 99%

Enrichment–Stowage–Cycle Strategy for Ultrasensitive Electrochemiluminescent Detection of HIV-DNA with Wide Dynamic Range

Wu¹,

Luo²,

Wen³

et al. 2019

Anal. Chem.

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Sensitive detection of human immunodeficiency virus DNA (HIV-DNA) is essential for timely diagnosis and cure of the illness. Herein, a novel “enrichment–stowage–cycle” strategy was proposed to fabricate a multiple amplified electrochemiluminecence (ECL) biosensor for HIV-DNA detection. On the basis of the enrichment role of magnetic nanobeads, assembly role of copolymer nanospheres and strand displacement amplification (SDA), the processes were named as “enrichment”, “stowage”, and “cycle”, respectively. The method employed electrochemiluminescent nanospheres (ENs) as signal labels by assembling three layers of CdSe/ZnS quantum dots (QDs) onto the surface of copolymer nanospheres. Compared to QDs, the same concentration of ENs can the enhance the ECL intensity by about 11.3-fold. SDA could further amplify the signals by about 3.77-fold, possessing high sensitivity for low-abundant biomarkers detection. The integration of magnetic separation improved detection specificity and stability, making the method possible for practical application. On the basis of magnetic separation, ENs and SDA, the ECL biosensor realized ultrasensitive detection of 39.81 fM HIV-DNA, which was more sensitive than other HIV-DNA analytical methods, with a wide dynamic range of 0.05 pM to 50 nM. The successful detection of HIV-DNA in complex samples with good sensitivity and accuracy indicated its potential utilization in early judgment of diseases and fabrication of signal amplification platforms.

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Magnetic electrochemiluminescent immunoassay with quantum dots label for highly efficient detection of the tumor marker α-fetoprotein

Cited by 22 publications

References 31 publications

Synthesis, Properties, and Biological Applications of Metallic Alloy Nanoparticles

Synthesis, Properties, and Biological Applications of Metallic Alloy Nanoparticles

Towards cancer diagnostics – an α-feto protein electrochemical immunosensor on a manganese(iv) oxide/gold nanocomposite immobilisation layer

Enrichment–Stowage–Cycle Strategy for Ultrasensitive Electrochemiluminescent Detection of HIV-DNA with Wide Dynamic Range

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