Magnetic extraction and purification of DNA from whole human blood using a γ-Fe2O3@Chitosan@Polyaniline hybrid nanocomposite

Abstract

We examined the properties of the nanocomposite γ-FeO@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV-vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (q) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-FeO@Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.

Eudragit E100/poly(ethylene oxide) electrospun fibers for DNA removal from aqueous solution

“…The typical procedure for development of DNA adsorbents is based on chemical modification of surfaces for incorporation of cationic groups for electrostatic interaction with DNA—negatively charged phosphate groups . With the aim of minimizing the complexity of overall process for DNA extraction, different strategies have been explored, such as from incorporation of cationic species on mica surfaces, production of magnetic nanocomposites of maghemite/polyaniline and maghemite/chitosan/polyaniline . However, the production of adsorbents with higher surface area associated with easier removal from water/biologic fluid is a new frontier to be explored, with the development of low‐cost prototypes of adsorbents, such as nano‐ and micro‐nonwoven fibers for scaffolds in tissue engineering, nucleic acid retention, and drug delivery systems …”

Biopolymers in sorbent-based microextraction methods

“…In most applications, magnetic chitosan sorbents were combined with other materials to prepare hybrid composites, such as GO [75,77,79e81,83,86,91] MIPs [60,63,69,70,78,80,81,88,92]; other polymers including polyaniline [59,66,68], polypyrrole [57,61,89], and polythiophene [62,64]; and even ILs [77], deep eutectic solvents [69,82], surfactants [71], and antibodies [84]. When dealing with magnetic GO/chitosan sorbents, the GO is added to the reaction solution during the crosslinking of chitosan in presence of the MNPs.…”

“…It is important to highlight the ability of chitosan-based sorbents to extract DNA with high purity from complex matrixes, such as blood and saliva, using m-m-dSPE methods [68,82,85], as well as using the m-SPE microchip prepared with a filter paper modified with chitosan [37]. These methods were combined with UV spectroscopy [82,85] or the polymerase chain reaction [37,78] for the quantification of the DNA.…”

Complexos de nanofibras eletrofiadas e ácidos nucleicos: Uma revisão

“…Assim, as fibras eletrofiadas poderiam integrar estratégias em processos de separação, no esforço para redução no número de etapas de extração e purificação de DNA de interesse visando sobretudo o desenvolvimento de novas abordagens de diagnóstico de doenças com fácil manipulação de material biológico (Habyarimana et al, 2018;Maciel et al, 2018;Tan & Yiap, 2009). Com o desenvolvimento da engenharia genética, há de se considerar também os riscos de transferência horizontal de genes ou "poluição gênica", sendo oportuno o desenvolvimento de matrizes para captura de DNA recombinante (Baquero, 2004;Panoff & Chuiton, 2004).…”