Magnetic Fe3O4-Based Sandwich-Type Biosensor Using Modified Gold Nanoparticles as Colorimetric Probes for the Detection of Dopamine

Abstract

In this work, we designed a visual biosensor for dopamine (DA) detection using magnetic Fe3O4 particles and dithiobis(sulfosuccinimidylpropionate)-modified gold nanoparticles (DTSSP-AuNPs) as the recognition elements. Specifically, DA molecules were assembled onto the surface of DTSSP-AuNPs via the amine coupling reaction between the amino group of DA and activated carboxyl group of DTSSP. Accordingly, DA-anchored DTSSP-AuNPs were captured by Fe3O4 through the interaction of catechol and iron. In a magnetic field, the formed Fe3O4-DA-DTSSP-AuNPs conjugates were easily removed from the solution, leading to fading of the AuNPs suspension and decrease of the UV/Vis signal. As a result, a detection limit of 10 nM for DA was achieved. The theoretical simplicity and high selectivity demonstrated that the sandwich-type strategy based on Fe3O4 and AuNPs would lead to many colorimetric detection applications in clinical study by rationally designing the surface chemistry of AuNPs and Fe3O4.

Advanced materials for optical sensing and biosensing of neurotransmitters

“…The decreased absorbance of the probe is linear in the range 0.02-0.80 µM (LOD, 10 nM). Considering that amine-reactive succinimidyl residues on the surfaces of AuNPs can react selectively with the primary amine group of DA, the selectivity of the probe is enhanced in the presence of other catechols and amino acids [64].…”

Rapid determination of dopamine in human plasma using a gold nanoparticle-based dual-mode sensing system

“…Several colorimetric methods based on the distance-dependent optical properties of AuNPs have been developed for dopamine detection. The color variation of AuNPs associated with the reversible process from dispersion to aggregation induced by the intermolecular interactions between dopamine and surface modifiers on the AuNPs can be observed by naked eyes and quantified by UV-vis spectra [18][19][20][21][22]. In addition, several fluorometric assays have been demonstrated for the detection of dopamine with high sensitivity using fluorophores such as calcein blue-Fe 2+ complex [23], carbon dots [24], silicon nanoparticles [25], molecule capped QDs [26][27][28][29], and gold nanoclusters (AuNCs) [30,31].…”

Ultrasensitive and Selective Detection of Dopamine Using Cobalt-Phthalocyanine Nanopillar-Based Surface Acoustic Wave Sensor

“…In the case of DA detection by commonly employed electrochemical methods, limitations are related to the fact that DA oxidized at almost the same potential as AA. Nevertheless, recent publications have highlighted several strategies to overcome this problem using new nanocomposites 5-10 , magnetic particles [11][12][13][14][15][16] , conducting polymer [17][18] , carbon nanotubes 19-25 and molecularly imprinted polymers as active layers [26][27][28][29][30] . However, the development of these electrochemical sensors requires yet large numbers of steps for fabrication.…”