Magnetic immuno capture PCR assay (MIPA): Detection of Leptospira borgpetersenii serovar hardjo

Taylor, Mark J.; Ellis, William; Montgomery, J.M.; Yan, Keding; McDowell, S.W.J.; Mackie, D.P.

doi:10.1016/s0378-1135(96)01351-x

Cited by 27 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, few have been shown to amplify leptospiral DNA from either human (247,386) or veterinary (378,559,594,606,643,670) clinical material, and of these, only two methods have been subject to extensive clinical evaluation (84,387). Both methods were found to be more sensitive than culture, but differences in analysis of the data render direct comparisons between the two approaches impossible.…”

Section: Molecular Diagnosismentioning

confidence: 99%

Leptospirosis

2001

View full text Add to dashboard Cite

Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

show abstract

Section: Molecular Diagnosismentioning

confidence: 99%

Leptospirosis

2001

View full text Add to dashboard Cite

show abstract

“…A real-time PCR method based on 16S rDNA was developed that could be used on samples without the need for prior isolation and culture [Smythe et al, 2002]. Another popularly used method [Gravekamp et al, 1993] [Taylor et al, 1997] that consists of the immuno-magnetic separation of leptospires from inhibitors in frozen formalin-fixed bovine urine prior to PCR detection that resulted in a marked improvement in the detection of leptospires in urine samples. PCR based on ompL1 [Reitstetter, 2006] …”

Section: Leptospiremic or Antigenic Phase: Culture Dfm And Pcrmentioning

confidence: 99%

Insights into Leptospirosis, a Neglected Disease

Sritharan¹

2012

Zoonosis

View full text Add to dashboard Cite

“…Since MAbs reacted with different serovars from serogroup Sejroe, the absence of reaction with serovar sejroe could be either due to expression of a different protein or lack of gene expression. An attempt to Production of Mabs specific for different serovars of pathogenic Leptospira have been reported by other authors (10,11). Although these MAbs can be used in the development of more specific diagnostic assays, their use is limited to the diagnosis of cases in which that particular serovar is involved.…”

Section: Resultsmentioning

confidence: 99%

“…Improvement of laboratory diagnosis of the disease has been attempted by detection of leptospiral antigens in clinical material from either humans or animals using methods such as immunofluorescence (2), radioimmunoassay and enzymelinked immunosorbent assay (1), polymerase chain reaction (PCR) (4), and immunomagnetic capture combined to PCR (11 lack specificity and PCR can be affected by inhibitory substances from the samples. Monoclonal antibodies (MAbs) directed against surface antigens of pathogenic Leptospira would be useful for the development of more specific assays.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira

Ludtke¹,

Coutinho²,

Jouglard³

et al. 2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and nonpathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted with non-pathogenic Leptospira. The MAbs described have potential for use in diagnostic tests for leptospirosis.

show abstract

Magnetic immuno capture PCR assay (MIPA): Detection of Leptospira borgpetersenii serovar hardjo

Cited by 27 publications

References 26 publications

Leptospirosis

Leptospirosis

Insights into Leptospirosis, a Neglected Disease

Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira

Contact Info

Product

Resources

About